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UBC 'megapixel' DNA replication technology promises faster, more precise diagnostics
Date:7/3/2011

sensitively and density over the original technique, developed in 1983. The greatest number of chambers available in commercially available implementations of digital PCR, using integrated micro-valves, is 36,960. However, further scalability is limited by the maximum density at which valves may be reliably fabricated.

Hansen believes the new version or digital PCR can be scaled to hold up to approximately 10,000,000 chambers on a standard one inch format.

The UBC researchers also found the new 'megapixel' technique set new benchmarks in detecting rare mutations--defined as the lowest measurable ratio of two target sequences differing by a single nucleotide variation as well as new limits in the detection of subtle differences in sequence abundance.

Partitioning of a one million chamber array takes approximately one minute.

"Our solution, or something using the same techniques, could enable a new degree of precision in measurements in biomedical research and diagnostics. The dramatic increase in assay density has important implications for the adoption of digital PCR as an economical, fast and routine analytical tool," says Hansen.


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Contact: Carl Hansen
chansen@phas.ubc.ca
604-827-3229
University of British Columbia
Source:Eurekalert

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