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UBC-led team develops platform to monitor hematopoietic stem cells
Date:5/22/2011

ques are labour intensive, require expensive reagents and provide limited flexibility to characterize cells or exchange culture conditions.

The team designed and fabricated microfluidic devices -- about the size of a matchbox -- containing 1,600 to 6,400 miniature culture chambers that can sustain robust cell growth, along with an automated time-lapse imaging system to track clones over multiple days as they expand from single cells.

"There are many challenges associated with the culture of suspension cells in nanolitre volumes including dehydration, nutrient limitations, and rapid variations if culture conditions are not well controlled," says Lecault.

The team was able to solve these problems by integrating an osmotic bath to block evaporation combined with a unique geometry that allows for automated medium exchange, immunostaining on live clones and cell recovery.


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Contact: Vronique Lecault
vlecault@chbe.ubc.ca
604-827-3547
University of British Columbia
Source:Eurekalert

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