Navigation Links
UBC-led team develops platform to monitor hematopoietic stem cells
Date:5/22/2011

A Canadian research team has developed an automated microfluidic cell culture platform to monitor the growth, survival and responses of hundreds of hematopoietic stem cells (HSCs) at the single cell level.

This new tool allows scientists to study multiple temporally varying culture conditions simultaneously and to gain new insights on the growth factor requirements for HSC survival.

"The ability to perform massively parallel cultures of single non-adherent mammalian cells will provide new avenues to explore complex biological questions," says Vronique Lecault, lead author of the study and a PhD candidate in the UBC Dept. of Chemical and Biological Engineering.

"Our results will find use in broader applications such as drug development, clone selection and culture optimization," says Lecault.

The findings appear in the May 22 issue of the online journal Nature Methods. The study is a collaborative project between the laboratories of Asst. Prof. Carl Hansen, UBC Physics and Astronomy, Centre for High-Throughput Biology, Prof. James Piret, UBC Chemical and Biological Engineering, Michael Smith Laboratories, Prof. Connie Eaves, Terry Fox Laboratory, BC Cancer Agency, and Dr. Keith Humphries, Terry Fox Laboratory, BC Cancer Agency.

Lecault explains that HSCs are found mainly in adult bone marrow and have the astounding ability to sustain the continuous production of specialized blood cells.

These cells have major clinical implications, in particular for the treatment of cancer and blood-borne diseases, but the mechanisms regulating their division into stem cells (self-renewal) or more mature cells (differentiation) are not very well understood.

The heterogeneous nature of hematopoietic populations further complicates the study of these rare HSCs by hiding individual responses into average measurements. Single cell studies are therefore critical to elucidate these mechanisms but current techniques are labour intensive, require expensive reagents and provide limited flexibility to characterize cells or exchange culture conditions.

The team designed and fabricated microfluidic devices -- about the size of a matchbox -- containing 1,600 to 6,400 miniature culture chambers that can sustain robust cell growth, along with an automated time-lapse imaging system to track clones over multiple days as they expand from single cells.

"There are many challenges associated with the culture of suspension cells in nanolitre volumes including dehydration, nutrient limitations, and rapid variations if culture conditions are not well controlled," says Lecault.

The team was able to solve these problems by integrating an osmotic bath to block evaporation combined with a unique geometry that allows for automated medium exchange, immunostaining on live clones and cell recovery.


'/>"/>

Contact: Vronique Lecault
vlecault@chbe.ubc.ca
604-827-3547
University of British Columbia
Source:Eurekalert

Related biology news :

1. Iowa State researcher develops new treatment method for canine eye diseases
2. Hypertension develops early, silently, in African-American men
3. MIT develops new way to fuse cells
4. Fujitsu Develops HDD Security Technology Based on Opal SSC Standards
5. When acute hepatitis develops into chronic hepatitis
6. MIT student develops new innovations to selectively kill cancer cells
7. CSHL team develops mouse models of leukemia that predict response to chemotherapy
8. HudsonAlpha investigator develops rapid response swine flu test
9. Carnegie Mellon develops innovative method to detect genetic causes of complex diseases
10. Epigentek Develops a New Method of Measuring Global DNA Methylation
11. GSU professor develops new method to help keep fruit, vegetables and flowers fresh
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/3/2016)... 3, 2016 Vigilant Solutions announces today that ... Missouri solved two recent hit-and-run ... data from Vigilant Solutions. Brian Wenberg ... the victim was walking out of a convenience store and witnessed an elderly ... his vehicle, striking his vehicle and leaving the scene.  ...
(Date:2/2/2016)... Feb. 2, 2016 Technology Enhancements Accelerate Growth of ... of the digital and computed radiography markets in ... and Indonesia (TIM). It provides ... size, as well as regional market drivers and restraints. ... market penetration and market attractiveness, both for digital and ...
(Date:2/1/2016)... smart wallet ( www.wocketwallet.com ) announces the launch of a new video ... Las Vegas , where Joey appeared at the Wocket ... Vegas , where Joey appeared at the Wocket booth to meet ... filmed at the Consumer Electronics Show (CES2016) in Las Vegas ... greet fans. --> --> The ...
Breaking Biology News(10 mins):
(Date:2/9/2016)... ... 2016 , ... The American Academy of Thermology (AAT) has announced ... AAT Member Certification Qualification Course for Technicians via a two part webinar on July ... a detailed review of hardware, software, and camera setup/operations, aligns with the in-person member ...
(Date:2/9/2016)... ... February 09, 2016 , ... PharmApprove ... for the National Organization for Rare Disorders (NORD). Dorman will lead PharmApprove efforts ... are heard throughout the drug regulatory review process. , “Adding Diane Dorman is ...
(Date:2/9/2016)... Feb. 9, 2016 This market research report ... and future prospects of the market in terms of ... companies engaged in the manufacture of microbiology culture media ... with a market snapshot providing the overall information of ... report. This section also provides the overall information and ...
(Date:2/9/2016)... , February 9, 2016 Three-Year Initiative ... Children to Take Part in Life-Changing Camp ... initiative designed to positively affect the lives of children born with ... --> SHPG ) is announcing a new initiative designed ... as well as the future of rare disease care. --> ...
Breaking Biology Technology: