Navigation Links
Two new weapons in the battle against bacteria
Date:2/13/2014

This news release is available in German.

Proteins are made up of a chain of amino acids and are vital for all cell processes. Proteases are among the most important types of protein. Like "molecular scissors", they cut other proteins at given positions and thereby execute important cell functions. By cutting the amino acid chains to the right length or breaking proteins apart they, for example, activate or deactivate proteins, decompose defective ones or switch signal sequences that serve to transport proteins to their proper position within a cell.

But proteases are important not only for human cells bacteria also rely on them. There are hardly any effective antibiotics left in the fight against pathogens like multi-resistant strains of Staphylococcus aureus bacteria or Mycobacterium tuberculosis which causes tuberculosis.

Researchers around the world are thus working ardently to find new ways of disarming the proteases in these strains to combat them. At the heart of this effort lies the so-called ClpP protease. It comprises 14 subunits and has a central regulatory function. The usual approach to deactivating this protease is to block all active centers of the ClpP. These are effectively the "cutting edges of the scissors", i.e. the portions of the protein that are responsible for breaking apart other proteins.

"However, the inhibitors used in the past have one decisive disadvantage," explains Stephan Sieber who heads the Chair for Organic Chemistry II at the Technische Universitaet Muenchen (TUM). "They don't permanently disarm the proteins, but only work for a few hours. On top of that, to be effective they must attack all active centers of the protein."

New strategies against bacteria

In collaboration with Professor Michael Groll, who heads the Chair for Biochemistry, Malte Gersch and Roman Kolb, doctoral candidates at Professor Sieber's chair, have succeeded in uncovering two completely new mechanisms that can be used to permanently deactivate these important bacteriological proteases in one case even without having to attack all active centers of the protein.

The first mechanism disrupts the arrangement of amino acids required for the cohesion of the protease subunits. As a result the protease breaks into two parts. The second acts directly on the core of the active center. It converts the amino acid that does the actual splitting into another kind of amino acid the "scissors" lose their edge and the protein is rendered inoperable. Both approaches inhibit the protease in completely novel ways and are thus very promising for the development of new forms of medication.

The scientists also found a whole series of inhibitors that initiate the two mechanisms. "Knowing the ways in which substances deactivate the proteases is a huge advance," says Gersch. "We can now optimize the substances and possibly also apply the principle to other proteases."

In further research, Gersch and Sieber plan to test their substances on living bacterial strains to determine if these are truly inhibited in growth and pathogenic effect. "Although the bacteria are not completely disarmed, they produce significantly fewer toxins that are conducive to inflammation," says Gersch. "The basic idea is that we give the immune system more time to handle the pathogens on its own while the formation of new resistances is suppressed."


'/>"/>

Contact: Dr. Andreas Battenberg
battenberg@zv.tum.de
49-892-891-0510
Technische Universitaet Muenchen
Source:Eurekalert  

Related biology news :

1. Dismantling Syrias chemical weapons in the midst of war
2. Weapons testing data determines brain makes new neurons into adulthood
3. DARPA director: Revolutionary weapons require strong S&T partnerships
4. Nuclear weapons surprising contribution to climate science
5. Effect of chronic exposure to chemicals used as weapons, pesticides under study
6. Poultry probiotics coat clues to ability to battle bugs
7. Battle against resistant bacteria takes huge leap forward
8. Predators vs. alien: European shrimps win predatory battles with an American invader
9. Helper cells aptly named in battle with invading pathogens
10. Tide is turning in skin cancer battle
11. A way of thinking may enable battle but prevent war crimes
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Two new weapons in the battle against bacteria
(Date:3/30/2017)... -- On April 6-7, 2017, Sequencing.com will host the world,s ... at Microsoft,s headquarters in Redmond, Washington ... health and wellness apps that provide a unique, personalized ... is the first hackathon for personal genomics and the ... the genomics, tech and health industries are sending teams ...
(Date:3/30/2017)... Trends, opportunities and forecast in this market ... (fingerprint, AFIS, iris recognition, facial recognition, hand geometry, vein ... use industry (government and law enforcement, commercial and retail, ... others), and by region ( North America ... Pacific , and the Rest of the World) ...
(Date:3/28/2017)... The report "Video Surveillance Market ... Storage Devices), Software (Video Analytics, VMS), and Service (VSaaS, ... to 2022", published by MarketsandMarkets, the market was valued ... to reach USD 75.64 Billion by 2022, at a ... year considered for the study is 2016 and the ...
Breaking Biology News(10 mins):
(Date:6/23/2017)... (PRWEB) , ... June 23, ... ... the launch of a redesigned, easier-to-navigate website for all six of their ... for physicians, nurses, dentists, pharmacists, physical and occupational therapists, and biotechnicians, DocCafe.com ...
(Date:6/22/2017)... ... June 22, 2017 , ... AESKU.GROUP, an ... Diagnostische Systeme & Technologien GmbH, thereby expanding its product portfolio to include allergy ... fever, urticaria, asthma, atopic eczema or a food allergy. Allergies are escalating to ...
(Date:6/22/2017)... MD (PRWEB) , ... June 22, 2017 , ... ... solutions provider, announced the release of Limfinity® version 6.5, a content-packed update to ... framework continue to gain a larger and more diverse base of customers among ...
(Date:6/20/2017)... 20, 2017  Kibow Biotech Inc., a pioneer in ... issuance of a new patent covering a unique method ... U.S. Patent and Trademark Office on May 23 rd ... Buzz of Bio award in 2014 in ... non-drug approaches to chronic disease. Renadylâ„¢, the first and ...
Breaking Biology Technology: