This study illustrates the value in using high through-put sequencing and microarray analysis to understand the fundamental properties of tumors at the molecular level, said Dr. Richard Gibbs, director of the BCM Human Genome Sequencing Center, and one of the papers co-authors. The identification of this gene demonstrates the power of copy number analysis using arrays (or gene chips).
The authors wrote: This study represents a step toward comprehensive genomic characterization for one of the most common lung cancers, lung adenocarcinoma. Recent advances in massively parallel DNA sequencing technology may soon make it feasible to undertake similar comprehensive studies to identify all translocations, point mutations and epigenomic changes in cancer and thus point the way to an integrated picture of the cancer genome. Dr. Barbara A. Weir of Dana-Farber and the Broad Institute, Michele S. Woo of Dana-Farber and Gad Getz of the Broad Institute are first authors on the paper and contributed equally to the work.
The team found 57 frequent genomic changes in their analysis of the genetics of tumors taken from lung cancer patients. Of these, 15 are linked to genes known to be involved in lung cancer. The rest remain to be discovered.
The gene NKX2-1 is essential in the development of cells that line the alveoli of the lungs. Mice lacking the gene die at birth because they cannot breathe. However, it is a proto-oncogene, which means that it can mutate into a gene that promotes development of cancer.
Lung adenocarcinoma is the leading cause of cancer death worldwide. Using gene chip or microarrays, researchers compared the genomes of 371 lung adenocarcinomas to 242 normal lung samples using special gene chips to analyze approximately 250,000 genetic markers. Their analyses identified areas of genetic material that has been repeated or deleted in the tumors. Among these were six areas currently associated with known mu
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Baylor College of Medicine