Navigation Links
Transport behavior of E. coli varies depending on manure source

MADISON, WI, MARCH 9, 2009 Escherichia coli is a commonly used indicator organism for detecting the presence of fecal contamination in drinking water supplies. The importance of E. coli as an indicator organism has led to several studies looking at the transport behavior of this important microorganism in groundwater environments. Commonly only a single strain of E. coli is used in these studies, yet research has shown that a significant amount of genetic variability exists among strains of E. coli isolated from different host species and even from the same host species. If these genetic differences result in differences in cell properties that affect transport, different strains of E. coli may exhibit different rates of transport in the environment.

A scientist at the USDA-ARS Animal Waste Management Research Unit in Bowling Green, Kentucky, in collaboration with researchers at the University of California at Riverside, compared cell properties and transport behavior of 12 different E. coli isolates obtained from six different fecal sources. Results from this study were published in the March-April issue of Journal of Environmental Quality.

For all 12 E. coli isolates, the following cell properties known to affect bacterial transport in the environment were measured: surface charge, hydrophobicity, cell size and shape, and the composition of the extracellular polymeric substance. Transport behavior of the E. coli isolates was assessed by measuring the amount of cells that were able to pass through columns packed with clean aquifer sands. The measured breakthrough concentrations of the bacteria were then modeled so that transport parameters for each E. coli isolate could be estimated. Correlations between measured cell properties and transport parameters were investigated.

Although each E. coli isolate was subjected to the exact same storage and growth conditions, the researchers observed a large range in measured cell properties, bacterial recovery, and fitted transport parameters for the different isolates. For example, cell hydrophobicity and surface charge were observed to vary by over an order of magnitude for the 12 different E. coli isolates. The total amount of bacteria passing through the sand columns ranged from less than 2% for one of the horse isolates to 95% for one of the beef cattle isolates and the fitted model parameters ranged by a factor of 50 for the different E. coli isolates. The only cell property observed to be statistically correlated with transport behavior of the E. coli isolates was cell width.

Carl Bolster, the lead scientist on the study, stated "This diversity in transport behavior must be taken into account when making assessments of the suitability of using E. coli as an indicator organism for specific pathogenic microorganisms in groundwater. In addition, our results suggest that the modeling of E. coli in the environment will likely require a distribution of bacterial attachment rates, even when modeling E. coli movement from a single fecal source."

Research is ongoing at USDA-ARS and UC Riverside to investigate the range in diversity in cell properties and transport behavior of E. coli under a variety of different experimental conditions; these include different growth conditions and types of sediment. Further research is needed to identify cell properties controlling E. coli transport in the environment.


Contact: Sara Uttech
Crop Science Society of America

Related biology news :

1. Mechanism for the in-vivo transport of siRNA
2. Conference on sustainable transportation is Oct. 29-31
3. Contribution of cholesterol transporter to vascular disease
4. Cornell researchers prove how plants transport sugars
5. Floating a big idea: MIT demos ancient use of rafts to transport goods
6. Montana State University researchers map iron transport protein
7. Livermore researchers use carbon nanotubes for molecular transport
8. UCLA researchers clarify function of glucose transport molecule
9. Protein transports nutrients believed to protect against eye disease
10. The viability of hydrogen transportation markets: Chicken or egg?
11. Simulations help explain fast water transport in nanotubes
Post Your Comments:
(Date:10/29/2015)... Mich. , Oct. 29, 2015  Rubicon ... Genomics for U.S. distribution of its DNA library ... kit and Rubicon,s new ThruPLEX Plasma-seq kit. ThruPLEX ... enable the preparation of NGS libraries for liquid ... for diagnostic and prognostic applications in cancer and ...
(Date:10/27/2015)... -- In the present market scenario, security is one ... verticals such as banking, healthcare, defense, electronic gadgets, and ... secure & simplified access control and growing rate of ... bank accounts, misuse of users, , and so on. ... and smartphones are expected to provide potential opportunities for ...
(Date:10/26/2015)... and LAS VEGAS , ... Nok Labs , an innovator in modern authentication and ... today announced the launch of its latest version of ... platform enabling organizations to use standards-based authentication that supports ... Nok S3 Authentication Suite is ideal for organizations deploying ...
Breaking Biology News(10 mins):
(Date:11/30/2015)... -- Champions Oncology, Inc. (CSBR), engaged in the development of ... and use of oncology drugs, today announced that ... at the LD MICRO Investor Conference on Wednesday, December ... conference, held at the Luxe Sunset Bel Air Hotel ... feature 200 small/micro-cap companies and is expected to host ...
(Date:11/30/2015)...  HUYA Bioscience International, the leader in accelerating global ... today announced it has signed a Memorandum of Understanding ... collaboration between KDDF and HUYA with the ultimate goal ... for the global market. China,s ... innovative preclinical and clinical stage compounds. The company advances ...
(Date:11/30/2015)... Germany , November 30, 2015 ... Vienna, Austria to be held December 1-4, ... in Vienna, Austria to be ... owned subsidiary of Vycor Medical, Inc. ("Vycor") (OTCQB: VYCO), announced ... Therapy Suite at the 3rd European Congress of NeuroRehabilitation ...
(Date:11/30/2015)... PETACH TIKVAH, Israel , Nov. 30, 2015 ... ), a leading developer of adult stem cell technologies for ... Therapeutics Ltd., has been awarded an additional grant of approximately ... Chief Scientist (OCS). This grant, the second this year, brings ... to approximately $1.8 million (approximately NIS7 million).  ...
Breaking Biology Technology: