Navigation Links
Transplanted genetically-modified adipose cells offer potential therapy for liver diseases
Date:12/20/2012

Putnam Valley, NY. (Dec. 20, 2012) Using mesenchymal stromal cells derived from adipose (fat) tissues, genetically modified to express a bioluminescent marker, researchers in Italy have tracked cells after transplantation. The cells were followed from their injection into the spleen of mice modeling liver disease, to their characterization as "hepatic precursors," and to their subsequent migration through the spleen before engrafting at regenerating sites in the liver by bioluminescent imaging.

Their study is described in a recent issue of Cell Transplantation (21:9), now freely available on-line at http://www.ingentaconnect.com/content/cog/ct/. It adds to the developments in cell transplantation that have the potential to offer an alternative to liver transplantation for patients with liver disease. It also increases the validation of the therapeutic potential for cell transplantation with cells other than hepatic cells,

"Liver transplantation is the major therapeutic option for patients affected by liver disease," said study co-author Dr. Gabriele Toietta of the Ospedale Pediatrico Bambino Gesu in Rome, Italy. "However, scarcity of organs, high costs and lifelong immunosuppressive treatment make the potential for transplanting hepatic precursor cells an attractive alternative. In addition, hepatocytes obtained from organs are generally not suitable for transplantation."

Historically, the isolation and preservation of hepatic cells from non-living liver donors has been a limiting factor impacting on organ transplantation. The advantage of using adipose tissue derived stromal cells (AT-SCs) comes from the great availability of fat cells and the ease of obtaining them. Also, the possibility of manipulating and transplanting autologous (self-donated) cells eliminates the need for lifelong immunosuppression treatments. However, in cases of genetically-caused liver disease, cells would have to be obtained from other donors than the patient (allogenic), and so would still require immunosuppressive therapy.

In this study, the researchers demonstrated that modified and transplanted AT-SCs were capable of migrating through the spleen, engrafting in the liver into regenerating sites, and persisting in the hepatic parenchyma for up to two months. In addition, the researchers were able to track the AT-SCs transplanted in their study.

"To our knowledge, this is the first use of bioluminescence imaging - which involves the detection of photons from cells expressing luciferase enzymes - to monitor an experimental approach of cell therapy using AT-SCs for liver disorders," said the researchers.

The researchers also discovered the activation of a "promoter" that suggested that the transplanted cells had a "commitment towards hepatogenic differentiation in vivo."

"Our data suggests engraftment and repopulation of injured livers by transplanted AT-SCs and we confirmed that AT-SCs differentiate towards hepatogenic-like characteristics both in vitro and in vivo," concluded the researchers. "In addition, we detected the activation of a promoter - normally silent in adult tissues - but can be re-activated during liver regeneration."


'/>"/>

Contact: Robert Miranda
cogcomm@aol.com
Cell Transplantation Center of Excellence for Aging and Brain Repair
Source:Eurekalert

Related biology news :

1. Transplanted neural stem cells slows als onset and progression in mouse models
2. Cell Transplantation study investigates fate and function of cells transplanted to the CNS
3. Tortoise and the hare: New drug stops rushing cancer cells, slow and steady healthy cells unharmed
4. Stem cells can repair a damaged cornea
5. Scientists produce eye structures from human blood-derived stem cells
6. Study demonstrates cells can acquire new functions through transcriptional regulatory network
7. Epigenetic signatures direct the repair potential of reprogrammed cells
8. Researchers print live cells with a standard inkjet printer
9. Nanopills release drugs directly from the inside of cells
10. Protein jailbreak helps breast cancer cells live
11. Newly found protein helps cells build tissues
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/31/2016)... R.I. , March 31, 2016  Genomics firm ... of founding CEO, Barrett Bready , M.D., who ... members of the original technical leadership team, including Chief ... President of Product Development, Steve Nurnberg and Vice President ... returned to the company. Dr. Bready served ...
(Date:3/22/2016)... and SANDY, Utah , ... which operates the highest sample volume laboratory in ... Genomics and UNIConnect, leaders in clinical sequencing informatics and ... launch of a project to establish the informatics infrastructure ... NSO has been contracted by the Ontario ...
(Date:3/15/2016)... March 15, 2016 --> ... by Transparency Market Research "Digital Door Lock Systems Market - ... - 2023," the global digital door lock systems market in ... 2014 and is forecast to grow at a CAGR of ... small and medium enterprises (MSMEs) across the world and high ...
Breaking Biology News(10 mins):
(Date:5/26/2016)... ... May 26, 2016 , ... FireflySci ... used in leading laboratories all over the globe. Their cute firefly logo has ... manufacturing awesome cuvettes, FireflySci makes spectrophotometer calibration standards that never require recalibration. ...
(Date:5/25/2016)... ... May 25, 2016 , ... Founder ... double board-certified in surgery and surgery of the hand by the National Board ... stranger to going above and beyond in his pursuit of providing the most ...
(Date:5/25/2016)... ... May 25, 2016 , ... Lady had been battling arthritis since the ... in her left knee. Lady’s owner Hannah sought the help of Dr Jeff Christiansen ... to repair her cruciate ligament and help with the pain of Lady’s arthritis. Dr ...
(Date:5/24/2016)... ... , ... Last week, Callan Capital, an integrated wealth management firm specializing in ... San Diego Life Science event at the Estancia La Jolla Resort and Spa. , ... speakers Dr. Rich Heyman, former CEO of Aragon and Seragon, and Faheem Hasnain, former ...
Breaking Biology Technology: