Navigation Links
Transcriptome analysis, organ culture methods featured in Cold Spring Harbor Protocols
Date:1/3/2011

COLD SPRING HARBOR, N.Y. (Mon., Jan. 3, 2011) -- New technologies and methods are spurring a renaissance in the study of organogenesis. Organogenesis, essentially the process through which a group of cells becomes a functioning organ, has important connections to biological processes at the cellular and developmental levels, and its study offers great potential for medical treatments through tissue engineering approaches. The January issue of Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/TOCs/toc1_11.dtl) features a method from Washington University's Hila Barak and Scott Boyle (http://devbio.wustl.edu/kopanlab/) for "Organ Culture and Immunostaining of Mouse Embryonic Kidneys." The kidney is particularly interesting as it also serves as a model for branching morphogenesis. The protocol describes the isolation, culture and fluorescent immunostaining of mouse embryonic kidneys, and is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2011/1/pdb.5558).

Cap analysis gene expression (CAGE) is a method used to discover new promoters and for quantifying gene activity, providing data essential for studies of regulatory gene networks. But CAGE requires large amounts of RNA, which are often not obtainable from rare specimens. In the January issue of Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/TOCs/toc1_11.dtl) Piero Carninci and colleagues from the RIKEN Yokohama Institute's Omics Science Center (http://www.osc.riken.jp/english/) present "NanoCAGE: A High-Resolution Technique to Discover and Interrogate Cell Transcriptomes," a method that can capture information from as little as 10 nanograms of total RNA. The protocol describes how to rapidly prepare nanoCAGE libraries which can be sequenced with high sensitivity. The article is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2011/1/pdb.prot5559).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. The developmental dynamics of the maize leaf transcriptome
2. University of the Basque Country researchers decode transcriptome for gray mullet
3. New genomic technique uncovers coral transcriptome
4. Precise trace gas analysis, without the noise
5. Study: Personal contacts at work help people better understand organ donation
6. Sinking organic materials produce carbon dioxide
7. New study into bladder regeneration heralds organ replacement treatment
8. Research links damaged organs to change in biochemical wave patterns
9. Swimming microorganisms stir things up, and the LHC takes over
10. WSU researchers taking sustainability of organic farming to new level
11. Is organic farming good for wildlife? It depends on the alternative
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/23/2017)... robotic gym for the rehabilitation and functional motor sense evaluation of lower ... . The first 30 robots will be available from June in ... The technology was developed and patented at the IIT laboratories and has ... to a 10 million euro investment from entrepreneur Sergio Dompè. ... ...
(Date:5/6/2017)... 5, 2017 RAM Group , ... new breakthrough in biometric authentication based on a ... properties to perform biometric authentication. These new sensors are ... created by Ram Group and its partners. This sensor ... supply chains and security. Ram Group is a ...
(Date:4/18/2017)...  Socionext Inc., a global expert in SoC-based imaging and computing ... M820, which features the company,s hybrid codec technology. A demonstration utilizing ... Inc., will be showcased during the upcoming Medtec Japan at Tokyo ... Las Vegas Convention Center April 24-27. ... Click here for an image ...
Breaking Biology News(10 mins):
(Date:6/22/2017)... Ca (PRWEB) , ... June 22, 2017 , ... The ... It took 20 years until the first data on cross-contamination of human cell lines ... has been an increasing issue in cell culture labs and is associated with dramatic ...
(Date:6/20/2017)... ... 20, 2017 , ... CTNext , Connecticut’s go-to resource ... a Higher Education Entrepreneurship Advisory Committee to implement the recommendations of the master ... representatives from 35 higher education institutions across the state over the past six ...
(Date:6/20/2017)... , June 20, 2017  Kibow Biotech Inc., a ... announce the issuance of a new patent covering a ... by the U.S. Patent and Trademark Office on May ... of the Buzz of Bio award in 2014 in ... to developing non-drug approaches to chronic disease. Renadyl™, the ...
(Date:6/20/2017)... ... June 20, 2017 , ... GigaGen Inc ., a ... repertoires, announces launch of its new Surge(TM) Discovery service at the ... GigaGen, will present on Surge at the conference. , Surge is the new, ...
Breaking Biology Technology: