Navigation Links
Transcriptome analysis, organ culture methods featured in Cold Spring Harbor Protocols
Date:1/3/2011

COLD SPRING HARBOR, N.Y. (Mon., Jan. 3, 2011) -- New technologies and methods are spurring a renaissance in the study of organogenesis. Organogenesis, essentially the process through which a group of cells becomes a functioning organ, has important connections to biological processes at the cellular and developmental levels, and its study offers great potential for medical treatments through tissue engineering approaches. The January issue of Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/TOCs/toc1_11.dtl) features a method from Washington University's Hila Barak and Scott Boyle (http://devbio.wustl.edu/kopanlab/) for "Organ Culture and Immunostaining of Mouse Embryonic Kidneys." The kidney is particularly interesting as it also serves as a model for branching morphogenesis. The protocol describes the isolation, culture and fluorescent immunostaining of mouse embryonic kidneys, and is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2011/1/pdb.5558).

Cap analysis gene expression (CAGE) is a method used to discover new promoters and for quantifying gene activity, providing data essential for studies of regulatory gene networks. But CAGE requires large amounts of RNA, which are often not obtainable from rare specimens. In the January issue of Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/TOCs/toc1_11.dtl) Piero Carninci and colleagues from the RIKEN Yokohama Institute's Omics Science Center (http://www.osc.riken.jp/english/) present "NanoCAGE: A High-Resolution Technique to Discover and Interrogate Cell Transcriptomes," a method that can capture information from as little as 10 nanograms of total RNA. The protocol describes how to rapidly prepare nanoCAGE libraries which can be sequenced with high sensitivity. The article is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2011/1/pdb.prot5559).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. The developmental dynamics of the maize leaf transcriptome
2. University of the Basque Country researchers decode transcriptome for gray mullet
3. New genomic technique uncovers coral transcriptome
4. Precise trace gas analysis, without the noise
5. Study: Personal contacts at work help people better understand organ donation
6. Sinking organic materials produce carbon dioxide
7. New study into bladder regeneration heralds organ replacement treatment
8. Research links damaged organs to change in biochemical wave patterns
9. Swimming microorganisms stir things up, and the LHC takes over
10. WSU researchers taking sustainability of organic farming to new level
11. Is organic farming good for wildlife? It depends on the alternative
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/28/2017)... , March 28, 2017 The ... Hardware (Camera, Monitors, Servers, Storage Devices), Software (Video Analytics, ... Region - Global Forecast to 2022", published by MarketsandMarkets, ... 2016 and is projected to reach USD 75.64 Billion ... and 2022. The base year considered for the study ...
(Date:3/24/2017)... 24, 2017 The Controller General of Immigration from ... Abdulla Algeen have received the prestigious international IAIR Award for the ... Continue Reading ... ... Controller Abdulla Algeen (small picture on the right) have received the IAIR ...
(Date:3/23/2017)... The report "Gesture Recognition and Touchless Sensing Market by Technology (Touch-based ... to 2022", published by MarketsandMarkets, the market is expected to be worth USD ... 2022. Continue Reading ... ... ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... and LAGUNA HILLS, Calif. , Oct. ... Cancer Research, London (ICR) and University ... SKY92, SkylineDx,s prognostic tool to risk-stratify patients with multiple myeloma ... MUK nine . The University of Leeds ... partly funded by Myeloma UK, and ICR will perform the ...
(Date:10/10/2017)... ... 2017 , ... San Diego-based team building and cooking events company, Lajollacooks4u, has ... The bold new look is part of a transformation to increase awareness, appeal to ... period. , It will also expand its service offering from its signature gourmet cooking ...
(Date:10/10/2017)... 10, 2017 International research firm Parks Associates announced ... at the TMA 2017 Annual Meeting , October 11 in ... residential home security market and how smart safety and security products impact ... Parks Associates: Smart Home ... "The residential security market has ...
(Date:10/9/2017)... DIEGO , Oct. 9, 2017  BioTech ... biological mechanism by which its ProCell stem cell ... critical limb ischemia.  The Company, demonstrated that treatment ... amount of limbs saved as compared to standard ... the molecule HGF resulted in reduction of therapeutic ...
Breaking Biology Technology: