Navigation Links
Too hot to handle! Scientists identify heat sensing regulator
Date:5/13/2008

Neuroscientists at Johns Hopkins are a step closer to understanding pain sensitivity - specifically why its variable instead of constant - having identified a gene that regulates a heat-activated molecular sensor.

Their description of the function of a membrane protein called Pirt appears in the May 2 issue of Cell.

Pain sensitivity increases during inflammation or injury and we want to know what molecules are involved in pain sensation when sensitivity is elevated, says Xinzhong Dong, Ph.D., an assistant professor of neuroscience at Hopkins.

The ability to sense temperature heat and spice is controlled by the TRPV1 protein channel found on the surface of certain nerve cells. In an inactive state, TRPV1 channels remain closed-there is no pain sensation. However, when noxious heat-temperatures above 108 degrees Fahrenheit-or capsaicin-the main ingredient in hot peppers-activates a TRPV1 channel, ions flow through, depolarizing the nerve to create an electrical current that sends pain signals to the brain.

The interesting thing about this channel is its not always constant, says Dong, whose team set out to find proteins that modulate TRPV1s action. They found the Pirt protein,phosphoinositide interacting regulator of TRP, and named it for its ability to regulate the TRPV1 channel.

To better understand how Pirt works, the researchers made mice that lacked Pirt and tested their ability to respond to heat. The mice were placed on a hot surface and monitored for how long it took them to scurry off. Mice lacking Pirt responded significantly slower than normal mice.

The team then exposed one hind paw to capsaicin and found that mice lacking Pirt did not lick their paws as long as normal mice, suggesting that without Pirt, they were compromised in their ability to sense the spice of capsaicin. The researchers also tried mustard oil on the hind paw and found mice lacking Pirt licked for about the same amount of time as normal mice. These observations suggest that Pirts action is specific to capsaicin and not other chemicals.

To figure out whether Pirt directly affects TRPV1 channel action, the researchers measured electrical currents generated by TRPV1 in single nerve cells with or without Pirt. They exposed some nerves to noxious heat-108 degrees Fahrenheit-and some nerves to capsaicin and compared currents generated in each cell. Cells containing Pirt generated stronger currents in heat and spice than cells lacking Pirt, leading the researchers to conclude that Pirt is required for a full pain response to both heat and spice.

Further research revealed that Pirt interacts with yet another molecule in the cell, a so-called acidic phospholipid, allowing access to TRPV1. According to Dong, through this phospholipid Pirt somehow changes the TRP channel, perhaps by opening it wider, or maybe by causing it to stay open longer. And the result is elevated pain sensitivity.

Exactly how Pirt regulates the TRPV1 channel isnt yet clear, says Dong. The goal is to find molecules that specifically affect the pain pathway, but not other nerves, he says. Were looking for genes specifically turned on in pain-sensing neurons. If we find them and can target them with new drugs, we will be able to treat pain without unfavorable side effects.


'/>"/>

Contact: Audrey Huang
audrey@jhmi.edu
410-614-5105
Johns Hopkins Medical Institutions
Source:Eurekalert

Related biology news :

1. Scientists dig deeper into the genetics of schizophrenia by evaluating microRNAs
2. Scientists endure Arctic for last campaign prior to CryoSat-2 launch
3. Scientists discover why plague is so lethal
4. UF scientists discover compound that could lead to new blood pressure drugs
5. UIC scientists discover how some bacteria survive antibiotics
6. Scientists aim to boost world energy supplies -- with microbes!
7. Scientists determine drug target for the most potent botulinum neurotoxin
8. Scientists make chemical cousin of DNA for use as new nanotechnology building block
9. Scientists find stem cells for the first time in the pituitary
10. Brown scientists say biodiversity is crucial to ecosystem productivity
11. Scientists urged to make a stand on climate change
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/30/2017)... -- The research team of The Hong Kong Polytechnic ... by adopting ground breaking 3D fingerprint minutiae recovery and matching technology, ... accuracy for use in identification, crime investigation, immigration control, security of ... ... A research team led by Dr ...
(Date:3/29/2017)... , March 29, 2017  higi, the health ... in North America , today announced ... and the acquisition of EveryMove. The new investment and ... set of tools to transform population health activities through ... lifestyle data. higi collects and secures data ...
(Date:3/27/2017)... ROCKVILLE CENTRE, N.Y. , March 27, 2017 ... by Healthcare Information and Management Systems Society (HIMSS) ... Analytics Outpatient EMR Adoption Model sm . In ... top 12% of U.S. hospitals using an electronic ... recognized CHS for its high level of EMR ...
Breaking Biology News(10 mins):
(Date:9/20/2017)... , ... September 20, 2017 ... ... a leading global provider of engineering, architecture, project controls, construction management, commissioning ... cleanrooms, today announced the unveiling of the iCON™ brand which represents the ...
(Date:9/20/2017)... (PRWEB) , ... September 20, 2017 , ... ... organization focused on molecular manufacturing and other transformative technologies, announced the winners for ... Experiment and the other for Theory in nanotechnology/molecular manufacturing. , Established in 1993 ...
(Date:9/19/2017)... ... September 19, 2017 , ... ... of funded early-stage tech companies. “Grit” author Angela Duckworth and her team at ... ic@3401 community is Cooley, an international law firm with decades of experience supporting ...
(Date:9/19/2017)... 19, 2017 ValGenesis Inc., the global leader ... to announce the strategic partnership with VTI Life Sciences ... validation services using the latest technology available in the ... clients with efficient and cost-effective validation services using ValGenesis ... the ValGenesis VLMS system. ...
Breaking Biology Technology: