Date:4/1/2010

mal. "Calcium Imaging of Neuronal Circuits In Vivo Using a Circuit-Tracing Pseudorabies Virus," from Lynn Enquist and colleagues at Princeton University (http://www.molbio1.princeton.edu/labs/enquist/), provides detailed instructions for the use of the pseudorabies virus (PRV) as a vector for imaging connectivity and activity of neuronal circuits. PRV has a broad host range but does not infect higher-order primates, and it travels along chains of synaptically connected neurons. The PRV strain used in this procedure encodes G-CaMP2, a sensitive fluorescent calcium sensor protein. Available in the April issue of Cold Spring Harbor Protocols (http://www.cshprotocols.org/TOCs/toc4_10.dtl), the method allows for reliable detection of endogenous circuit activity at single-cell resolution. As one of April's featured articles, it is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/4/pdb.prot5410).

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Contact: David Crotty crotty@cshl.edu 516-422-4007 Cold Spring Harbor Laboratory Source:Eurekalert

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