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Tips from the journals of the American Society for Microbiology
Date:6/19/2009

however, they lack the RNA required for virus replication.

In this study researchers generated VLPs from the structural proteins of the 1918 H1N1 virus and compared their ability to protect mice and ferrets against a reconstructed 1918 virus and the highly pathogenic avian H5N1 virus that was isolated from a fatal human case. When immunized twice intranasally with H1N1 VLPs mice were highly protected against a lethal challenge with both the 1918 and H5N1 virus. In contrast, mice receiving two intramuscular immunizations of 1918 VLPs were only protected against the 1918 viral challenge. Mucosal vaccination with 1918 VLPs induced higher levels of cross-reactivity in mice and complete survival in ferrets challenged with a lethal dose of the H5N1 virus. Only a 50% survival rate was noted in intramuscularly immunized animals.

"These results suggest a strategy of VLP vaccination against a pandemic virus and one that stimulates heterotypic immunity against an influenza virus strain with threatening pandemic potential," say the researchers.

(L.A. Perrone, A. Ahmad, V. Veguilla, X. Lu, G. Smith, J. M. Katz, P. Pushko, T.M. Tumpey. 2009. Intranasal vaccination with 1918 influenza virus-like particles protects mice and ferrets from lethal 1918 and H5N1 influenza virus challenge. Journal of Virology, 83. 11: 5726-5734.)


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Contact: Carrie Slijepcevic
cslijepcevic@asmusa.org
American Society for Microbiology
Source:Eurekalert

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