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Tips from the Journals of the American Society for Microbiology
Date:4/25/2008

eaf age, however the colonization was consistently 10-fold higher on the young (inner) leaves than on the middle leaves. Growth rates of S. enterica were found to be similarly leaf age dependent. Both bacterial pathogens also displayed higher population rates on younger leaves than on middle leaves harvested from mature lettuce heads.

Our results indicate that leaf age and nitrogen content contribute to shaping the bacterial communities of preharvest and postharvest lettuce and that young lettuce leaves may be associated with a greater risk of contamination with E. coli O157:H7.

(M.T. Brandl, R. Amundson. 2008. Leaf age as a risk factor in contamination of lettuce with Escherichia coli O157:H7 and Salmonella enterica. Applied and Environmental Microbiology, 74. 8: 2298-2306.)


New Method Simultaneously Tests for Fifteen Respiratory Viruses

A new test capable of simultaneously detecting 15 respiratory viruses may allow for quicker diagnosis and treatment of potentially deadly respiratory infections in children and adults worldwide. The researchers from The Netherlands report their findings in the April 2008 issue of the Journal of Clinical Microbiology.

Acute respiratory infections (RTIs) are responsible for high rates of morbidity and mortality throughout the world, however the cause remains inconclusive in over half of all reported cases. Respiratory viruses are believed to be among the pathogens contributing to a significant number of undiagnosed infections, but clinical presentation of patients with RTIs are generally not pathogen specific. While cell culture is still considered the gold standard for laboratory detection of respiratory viruses, slow response time and low sensitivity are suboptimal for routine detection practices.

In the study a new multiparameter test called RespiFinder was developed to detect 15 respiratory viruses in one reaction. Researchers then used the RespiFind
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Contact: Carrie Slijepcevic
cslijepcevic@asmusa.org
American Society for Microbiology
Source:Eurekalert

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