Navigation Links
This faster-growing E. coli strain's a good thing
Date:11/11/2010

URBANA A University of Illinois metabolic engineer has improved a strain of E. coli, making it grow faster. Don't worry, he believes his efforts will benefit human health, not decimate it.

"The average person hears E. coli and thinks of E. coli 0157:H7, a microorganism that causes horrific food poisoning, but we've developed a strain of E. coli that is suitable for mass production of high-quality DNA that could be used in vaccines or gene therapy," said Yong-Su Jin, a U of I assistant professor of microbial genomics and a faculty member in its Institute for Genomic Biology.

According to Jin, industrial strains of E. coli have already been used to produce such diverse products as insulin for diabetics, enzymes used in laundry detergent, and polymer substitutes in carpets and plastic.

"E. coli bacteria have contributed vastly to our scientific understanding of genes, proteins, and the genome as a model system of biology research," he added.

Jin worked with E. coli DH5α, a laboratory strain that had excellent potential but grew very slowly.

When scientists began to use E. coli DH5α in biotechnological research years ago, they handicapped it, causing some of the genes to mutate so it would meet the requirements of molecular biology experiments. There was a trade-off, thoughthe strain's slow growth in minimal media, commonly used in laboratory and industrial fermentations.

"E. coli DH5α has been so popular that scientists have used it to perform most recombinant DNA techniques. But its slow growth has been a critical weakness," Jin noted.

Because scientists had used random mutagenesis, they weren't sure where the mutation that caused the slow growth had occurred. Jin and his colleagues were able to locate and fix the problem.

"We learned that the scientists had unintentionally weakened a key enzyme in a gene in the nucleotide biosynthesis pathway. When we reversed this mutation, the modified strain grew as quickly as other types of E. coli used in industry while retaining the traits that make it useful in scientific experiments," he said.

The beauty of the new strain lies in the purity and abundance of the DNA that it contains, which makes it a candidate for use in important biotechnological applications, he said.

"For example, to make DNA vaccines and perform gene therapy, we need DNA that is extremely clean and pure. The E. coli strain we have developed is an excellent candidate to deliver this high-quality genetic material in large quantities," he said.


'/>"/>

Contact: Phyllis Picklesimer
p-pickle@illinois.edu
217-244-2827
University of Illinois College of Agricultural, Consumer and Environmental Sciences
Source:Eurekalert

Related biology news :

1. Researchers recreate SARS virus, open door for potential defenses against future strains
2. Novel bacterial strains clear algal toxins from drinking water
3. Genomes of 2 popular research strains of E. coli sequenced
4. Unexpectedly high rate of multiple strains in fungal infection
5. Scripps research scientists determine structure of immune molecule that counteracts HIV strains
6. Galloping and breathing at high speed
7. Meat-eating dinosaur from Argentina had bird-like breathing system
8. New meat-eating dinosaur from Argentina had bird-like breathing system
9. Migratory moths may hitch their rides, but theyre anything but drifters
10. Swamping bad cells with good in ALS animal models helps sustain breathing
11. OSAs ISP launches with research on breathing disorders and congenital heart defects
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/15/2016)... Puerto Rico , Jan. 15, 2016 ... big and small to find new ways to ensure ... culture. iOS and Android ... based on biometrics, transforming it into a hardware authorization ... that users swipe their fingerprint on their KodeKey enabled ...
(Date:1/11/2016)... 2016 Synaptics Incorporated (NASDAQ: SYNA ), ... that its ClearPad ® TouchView ™ 4300 ... separate categories in the 8 th Annual Mobile ... Breakthrough. The Synaptics ® TDDI solution enables faster ... thinner devices, brighter displays and borderless designs. ...
(Date:1/8/2016)... -- NXTD ), a company focused on ... privately held leading direct seller of vacation and entertainment ... company announced that on December 31, 2015, that WorldVentures ... to develop a proprietary new wireless smart card for ... unique smart wallet that serves to securely store all ...
Breaking Biology News(10 mins):
(Date:2/8/2016)... 2016 Novan, Inc. today announced that Director Robert ... Directors of Novan. In addition, Robert Keegan has been ... Carolina . --> North Carolina ... received a total of $32.8 million of net proceeds in a ... network originating throughout the Research Triangle area of North ...
(Date:2/8/2016)... , Feb. 8, 2016  BioElectronics Corporation (OTC ... devices, announced today that it is responding to ... from the Securities and Exchange Commission posted on ... , Chairman of the Board of BioElectronics Corporation ... Business Administration at The Fuqua School of Business, ...
(Date:2/8/2016)... , ... February 08, 2016 , ... ... wide range of loose, bulk foods at various stages of the production process. ... used to inspect large bulk products post packaging such as sacks of dry ...
(Date:2/8/2016)... SHELTON, Conn. , Feb. 8, 2016  NanoViricides, Inc. (NYSE ... that its CEO, Eugene Seymour , MD, MPH, will present ... 5:30PM at the Waldorf-Astoria Hotel in New York City ... presentation will be in the Windsor Room at 5:30PM EST. Registered ... New York City . --> ...
Breaking Biology Technology: