Recycling or "scrap press": physicians at the Ruhr-Universitt have found out which molecular mechanisms decide about the fate of the import receptor Pex18. Pex18 is responsible for the import of proteins into specific cell components, namely peroxisomes. Two opposing regulatory circuits determine whether the receptor remains active or is broken down after the transport has been completed. "Thus, the picture of the regulation of the protein import into peroxisomes has been completed and integrated to form one single model," says Junior Professor Dr Harald Platta from the RUB Faculty of Medicine. Together with Prof Dr Ralf Erdmann and other colleagues he reports in the journal "Traffic".
Ubiquitin signals determine the fate of the receptors
Because they don't have their own DNA, peroxisomes have to import all proteins that are necessary for them to fulfil their function. For this purpose, the cell is equipped with dynamic import receptors such as Pex18. They bind proteins in the cytoplasm and transport them to the peroxisome. The RUB team had demonstrated in a previous study that the signal protein ubiquitin subsequently decides about the future fate of the receptors: if a single ubiquitin protein docks with the receptor, the receptor gets recycled; it migrates back into the cytoplasm and launches a new transport process. If an ubiquitin chain docks with the receptor, a signal is sent out for the receptor to be broken down by the proteasome, an "intracellular scrap press", so to speak. Prior to this discovery, it had not been understood in what way the cell determines on the molecular level what happens to the receptor.
Recycling or "scrap press": It all depends on the enzyme cascade
The RUB physicians found out that different enzyme cascades catalyse the two ubiquitin modifications of Pex18. In both cases, it is a three-step process: the E1 enzyme activates the ubiquitin signal which is subsequently tran
|Contact: Dr. Harald W. Platta|