Navigation Links
The value of museum collections for development of DNA barcode libraries
Date:12/30/2013

d deposited in the Naturalis Biodiversity Center in Leiden were selected. For each target species, a series of increasingly older specimens was selected and brought to the lab for DNA sequencing. This was supplemented with freshly collected material representing nearly 150 Dutch spider species. The scientists recorded which specimens successfully produced DNA barcode sequences and which failed. They also experimented with DNA extraction techniques.

Typically, DNA extraction begins with the removal of muscle tissue; this is destructive extraction. An alternative approach is to soak the specimen in a solution that releases DNA from cells but does little or no damage to anatomy; this is nondestructive extraction. They found that failure rates for DNA barcode sequencing rise with time since collection, but body size is also a significant factor.

For freshly collected specimens overall, body size is not a predictor of sequencing success or failure. But larger species have a longer DNA barcoding shelf life than smaller species. Nondestructuve extraction techniques can significantly improve the chances of obtaining a DNA barcode sequence. Considering only the commonly applied destructive extraction method, small spiders are useful for only a few years while those with a body length of around 3 mm or more have a good chance of yielding a barcode sequence for about 20 years after collection.

But using nondestructive extraction, even small spiders with a body length of 4 mm or less have a good chance of yielding a DNA barcode sequence for about 15 years after collection while spiders above this size can yield barcode sequences for a considerably longer time. The success of nondestructive extraction demonstrated here coupled with the need to preserve museum specimens for a variety of research purposes bodes well for museum collections are source material for DNA barcode libraries.


'/>"/>

Contact: Jeremy A. Miller
jeremy.miller@naturalis.nl
Pensoft Publishers
Source:Eurekalert  

Page: 1 2

Related biology news :

1. West coast lumber and log exports increased in value, volume in third quarter of 2013
2. Study challenges soil testing for potassium and the fertilizer value of potassium chloride
3. People dont put a high value on climate protection
4. Stanford scientists use Flickr photos to assess value of natural tourist attractions
5. UCSF receives $4.5M to study value of gene sequencing in newborns
6. Calculating the value of effortful behavior: A clue to schizophrenia-related disability?
7. New research shows that potatoes provide one of the best nutritional values per penny
8. US urban trees store carbon, provide billions in economic value
9. Marine diversity study proves value of citizen science
10. Controversial dam removals founded on value conflicts
11. Study confirms prognostic value of new IASLC/ATS/ERS adenocarcinoma sub-classification
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
The value of museum collections for development of DNA barcode libraries
(Date:8/12/2015)... JOSE, Calif. , Aug. 12, 2015 /PRNewswire/ ... developer of human interface solutions, today announced that ... recently announced Match-in-Sensor solution, have officially been named ... Alliance. As part of the certification, Synaptics, Natural ... FIDO 1.0 Universal Authentication Framework (UAF) standard and ...
(Date:8/6/2015)... and TELTOW, Germany , August 6, ... Conference, SensoMotoric Instruments (SMI) shows the world,s ... , based on Epson,s Moverio BT-200 see-through head ... With this new solution, unprecedented quality and efficiency is ... with context-sensitive displays. For the first time, professionals and ...
(Date:8/4/2015)... 2015 Research ... the addition of the "Digital Forensics ... Sub-Segment (Computer Forensics, Network Forensics, Mobile Device ... Vertical and Region - Global Forecast to ... By Component (Hardware, Software, and Service), ...
Breaking Biology News(10 mins):Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 3SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 3Digital Forensics Market - The Market Represents a Compound Annual Growth Rate (CAGR) of 11.3% From 2015 to 2020 2
... Morphology: Fundamentals and Applications (CRC Publisher, 2009), a ... Frank Y. Shih http://web.njit.edu/shih offers a ... their relation to image processing. More than merely ... places this knowledge into a theoretical framework. Morphology ...
... sophisticated electric ion propulsion system has been switched on ... milestone in the satellite,s post-launch commissioning phase. The ... controlling the satellite,s orbit and speed. The push from ... the tiny amount of drag generated by the few ...
... Applications (CRC Publisher, 2009), a new reference book ... http://web.njit.edu/shih offers a comprehensive overview of ... image processing. More than merely a tutorial on ... into a theoretical framework. Morphology is the identification, ...
Cached Biology News:Image processing and mathematical morphology in new text by NJIT professor 2GOCE's electric ion propulsion engine switched on 2Image processing and mathematical morphology in new text by NJIT prof 2
(Date:8/26/2015)... , August 26, 2015 Israel Proves ... DiaCardio wins 1 st place  Wayerz comes in ... Israel by venture capital fund JVP, with ... the US, China , Israel ... .  Impressive achievement for the Israeli representatives in ...
(Date:8/26/2015)... Boston, MA (PRWEB) , ... August 26, 2015 , ... ... venue for Boston adult stem cell technology start-up company Asymmetrex to share ... widely the capabilities of a new biomarker for counting adult tissue stem cells. , ...
(Date:8/25/2015)... Calif. (PRWEB) , ... August 25, 2015 , ... One ... Valley -based Resilinc Corp., Bindiya Vakil, has been on a mission for ... the impact on global supply chain operations - from the two massive explosions that ...
(Date:8/25/2015)... PRUSSIA, Pa. , Aug. 25, 2015   ... has been enrolled in its Phase II/III clinical study ... company,s recombinant fusion protein linking coagulation factor VIIa with ... hemophilia A or B who have developed an inhibitor ... study will enroll approximately 54 male patients, the first ...
Breaking Biology Technology:Israeli Startup Takes First Place at Shengjing Global Innovation Awards 2015 2Israeli Startup Takes First Place at Shengjing Global Innovation Awards 2015 3Israeli Startup Takes First Place at Shengjing Global Innovation Awards 2015 4Israeli Startup Takes First Place at Shengjing Global Innovation Awards 2015 5Israeli Startup Takes First Place at Shengjing Global Innovation Awards 2015 6Asymmetrex Reports Positive Indicators for Crowdsourcing Campaign to Evaluate New Biomarker for Counting Adult Tissue Stem Cells for the First Time 2Asymmetrex Reports Positive Indicators for Crowdsourcing Campaign to Evaluate New Biomarker for Counting Adult Tissue Stem Cells for the First Time 3Asymmetrex Reports Positive Indicators for Crowdsourcing Campaign to Evaluate New Biomarker for Counting Adult Tissue Stem Cells for the First Time 4Resilinc Supply Chain Expert Warns: Impact of Tianjin Chemical Explosions Will be Far Worse than Reported 2Resilinc Supply Chain Expert Warns: Impact of Tianjin Chemical Explosions Will be Far Worse than Reported 3Resilinc Supply Chain Expert Warns: Impact of Tianjin Chemical Explosions Will be Far Worse than Reported 4CSL Behring Enrolls First Patient in Global Phase II/III Study of rVIIa-FP for On-Demand Treatment of Patients with Hemophilia A or B with Inhibitors 2CSL Behring Enrolls First Patient in Global Phase II/III Study of rVIIa-FP for On-Demand Treatment of Patients with Hemophilia A or B with Inhibitors 3CSL Behring Enrolls First Patient in Global Phase II/III Study of rVIIa-FP for On-Demand Treatment of Patients with Hemophilia A or B with Inhibitors 4
... NEW DELHI, India, August 29, 2011 , ... in September Bring focus on auto components, home ... on business opportunities for Indian SMEs at these global platforms ... a huge opportunity in global markets for Indian Small and ...
... Corporation (NYSE: WAT ) today recognized ... Research Institute (TGen) as a Waters Center ... In particular, Waters recognized Dr. Konstantinos Petritis, Head of ... leadership in the research of proteomics and biomarker discovery ...
... 2011 Emmy® and Tony® award-winning actress Blythe Danner ... Act 2 Reduce Fractures™, an educational campaign for women ... this year, Danner was diagnosed with postmenopausal osteoporosis at ... like her to educate themselves, take action and speak ...
Cached Biology Technology:IndiaMART.com to Step Up B2B Matchmaking at Global Trade Events 2IndiaMART.com to Step Up B2B Matchmaking at Global Trade Events 3Center for Proteomics at the Translational Genomics Research Institute (TGen) Selected as Waters Center of Innovation 2Center for Proteomics at the Translational Genomics Research Institute (TGen) Selected as Waters Center of Innovation 3Center for Proteomics at the Translational Genomics Research Institute (TGen) Selected as Waters Center of Innovation 4Award-Winning Actress Blythe Danner and Amgen Launch Act 2 Reduce Fractures™ Educational Campaign 2Award-Winning Actress Blythe Danner and Amgen Launch Act 2 Reduce Fractures™ Educational Campaign 3Award-Winning Actress Blythe Danner and Amgen Launch Act 2 Reduce Fractures™ Educational Campaign 4Award-Winning Actress Blythe Danner and Amgen Launch Act 2 Reduce Fractures™ Educational Campaign 5Award-Winning Actress Blythe Danner and Amgen Launch Act 2 Reduce Fractures™ Educational Campaign 6Award-Winning Actress Blythe Danner and Amgen Launch Act 2 Reduce Fractures™ Educational Campaign 7
... the template-directed polymerization of nucleotides into duplex ... I possesses a 3?5 exonuclease activity or ... during DNA replication, and also contains a ... to replace nucleotides in the growing strand ...
Request Info...
supplied with 10x reaction buffer...
... ATP: D-hexose 6- phosphotransferase/D-glucose-6-phosphate: NADP ... of HK will phosphorylate 1 ... min at 25C at pH ... will oxidize 1 mol of ...
Biology Products: