Navigation Links
The structure of the Mre11 protein bound to DNA
Date:10/3/2008

nd protein complexes are much more stable at room temperature than human proteins would be. For example, the extremophile Pyrococcus furiosus is an archaeal microorganism that grows happily at the temperature of boiling water; at room temperature, P. furiosus's proteins and their complexes are so stable they might as well be frozen.

Synapses and branching forks

Scott Williams and Gabriel Moncalian of the Scripps Institute's Department of Molecular Biology and the Skaggs Institute for Chemical Biology crystallized and determined x-ray crystal structures of key domains of P. furiosus Mre11 bound to DNA in two configurations, representing two kinds of DSB-repair interactions.

At beamline 12.3.1 at Berkeley Lab's Advanced Light Source, known as SIBYLS ("Structurally Integrated BiologY for Life Sciences"), the researchers performed both high-resolution protein crystallography and small-angle x-ray scattering (SAXS), which can image proteins and complexes in aqueous solution, closer to their natural state.

"Like breaking a piece of wood into two parts, the severed termini of the DNA in a double-strand break have ends of varying shape," says Williams. "The high-resolution snapshots we obtained of Mre11 binding to different DNA end-structures show how the Mre11 protein has evolved to hone in on and sense these variable mutagenic lesions."

Of the two complexes that were crystallized, the "synaptic complex" bound the ends of a pair of DNA helices in orientations mimicking the kind of break that might result when a double strand of DNA is severed by radiation or chemical damage.

A different form of double-strand break is also common and occurs when a DNA helix is forking or "unzipping" during replication; if the process is interrupted, the fork may collapse. This "branched complex" of DNA bound with Mre11 involves different topography and interactions than the synaptic complex.

W
'/>"/>

Contact: Paul Preuss
paul_preuss@lbl.gov
510-486-6249
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4 5 6

Related biology news :

1. Structures of important plant viruses determined
2. Carbon nanostructures form the future of electronics and optoelectronics
3. CCNY receives $5 million NSF grant to establish center for nanostructure applications
4. Atomic structure of the mammalian fatty acid factory determined
5. Researchers study facial structures, brain abnormalities to reveal formula for detection of autism
6. Improved technique determines structure in membrane proteins
7. Novel structure proteins could play a role in apoptosis
8. Scripps research scientists reveal key structure from ebola virus
9. Mini subs to probe odd structures in BC lake
10. Nature paper describes technique for extracting hierarchical structure of networks
11. Researchers reveal structure of protein that repairs damage to cancer cells
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
The structure of the Mre11 protein bound to DNA
(Date:12/10/2014)... , Dec. 9, 2014  Wake Forest Baptist Medical Center ... for its School of Medicine. Funding for this $50 ... campaign that will be publicly launched next summer. ... in the former 60 series R.J. Reynolds Tobacco Company ... Construction will begin immediately with plans to be ready ...
(Date:12/5/2014)... -- Tute Genomics, a leader in cloud-based solutions for genome ... led by UK-based Eurovestech. Peak Ventures and a number ... "We are at a pivotal time in ... seeks new approaches for the diagnosis, treatment and prevention ... and CEO of Tute Genomics. "One of the main ...
(Date:11/21/2014)... 2014  Earlier this year Donald Spector , ... one of the most prolific inventors in the world, ... from Smartphones to third party agencies. Spector envisioned this ... of the earliest known patents in this area. Now ... military, child care, elder care and hospital applications. This ...
Breaking Biology News(10 mins):Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4
... abyssal plain, miles below the ocean surface, don,t usually get much ... drift of mucus, fecal pellets, and body partsthat sinks down from ... fact that, over the long term, the steady fall of marine ... microbes living in the sediment. A new paper by MBARI researcher ...
... By integrating high-resolution X-ray imaging (termed microCT), 3D ... conducted by Carole Gee at the University of ... destroying the material. Traditional techniques, such as thin-sectioning, ... in order to observe internal structures. Dr. Gee, ...
... father,s cocaine use may make his sons less sensitive to ... suggests new findings from an animal study presented by Penn ... Society for Neuroscience. The study, led by Mathieu ... Christopher Pierce, PhD, associate professor of Neuroscience in Psychiatry at ...
Cached Biology News:Feast and famine on the abyssal plain 2Feast and famine on the abyssal plain 3Visualizing the past: Nondestructive imaging of ancient fossils 2Sons of cocaine-using fathers may resist addiction to drug, Penn Medicine study suggests 2
(Date:12/22/2014)... 2014 ITRA Global, one of the ... commercial real estate, has further expanded its global reach ... and Brisbane, Western Australia, reports Mylinda Vick, CCIM, Chairman ... ITRA Global / ACORPP (Australian Corporate Property and Projects) ... providing property services to a wide range of clients ...
(Date:12/19/2014)... December 19, 2014 Charm Sciences is ... for the Detection of Aflatoxin M1 in raw commingled ... independent third party validation. The peer reviewed report of ... the Institute for Agricultural and Fisheries Research (ILVO-T&V) has ... , Aflatoxin B1, the most toxic aflatoxin and a ...
(Date:12/19/2014)... , Dec. 19, 2014  Roche (SIX: RO, ROG; ... Technologies, Inc. (Bina), a privately held company based ... USA. Bina provides a big data platform for ... (NGS) data. Bina,s proprietary on-market Genomic Management Solution, ... researchers to perform fast and scalable analyses to ...
(Date:12/19/2014)... 19, 2014 Research and Markets ... the "Technology Innovations in Smart Fabrics (Technical ... http://photos.prnewswire.com/prnh/20130307/600769 The ... offers a detailed assessment on technological advancements and ... 1. The Smart Fabrics market ...
Breaking Biology Technology:ITRA Global Continues International Expansion in Perth and Brisbane, Australia 2Charm Sciences Achieves Independent Validation of First 15 Minute Quantitative Screening Method for Aflatoxin M1 2Roche acquires Bina Technologies and enters the genomic informatics market 2Roche acquires Bina Technologies and enters the genomic informatics market 3Technology Innovations in Smart Fabrics (Technical Insights) 2
... Oct. 18 Pharmion Corporation,(Nasdaq: PHRM ) will ... 31, 2007, after the close of the U.S. financial ... live audio,webcast to discuss the company,s results in greater ... 31, 2007. This call is being webcast by ...
... CLARA, Calif., Oct. 18 Coherent, Inc.,(Nasdaq: COHR ... after the market close and host a live webcast ... Wednesday, November 7,2007 at 4:30 p.m. ET. The ... can be accessed,at either http://www.coherent.com/Investors/ or ...
... ATLANTA, Oct. 18 CryoLife, Inc. (NYSE: ... company, announced,today that it will conduct its annual ... at the Company,s corporate headquarters,training facility in Kennesaw, ... surgical techniques of aortic valve replacement and,thoracoabdominal aortic ...
Cached Biology Technology:Pharmion Corporation to Announce Third Quarter 2007 Financial Results 2CryoLife to Conduct Annual Cardiac Surgery Fellows Allograft Symposium 2
Rabbit polyclonal to Vitronectin ( Abpromise for all tested applications). Antigen: Full length native Vitronectin (Cow) Entrez Gene ID: 7448 Swiss Protein ID: P04004...
... Competitor Assay Kits are ideal for studying ... PolarScreen GR Competitor Assay Kits contain partially ... one of the following proprietary fluorescent glucocorticoid ... Red (red assay kit), or GS Far ...
... to PRMT5 Arginine methylation is an ... recently been linked to protein activity. At ... been identified in mammalian cells. These enzymes ... functions by methylation of key proteins in ...
... coli Biotinylation Kit, Plasmid is designed for use ... expression kits. (Cat. No. 3 186 148, 3 ... vectors for the easy introduction of a C- ... can be enzymatically mono-biotinylated by E. coli biotin ...
Biology Products: