Navigation Links
The nano-channel that disentangles knots
Date:2/20/2013

The DNA, just like hair, has a tendency to become knotted, thus it may be useful to disentangle it.

Unfortunately, it is not possible to "actively" choose at random (or better, in one solution) the filaments with the desired features, and this is why scientists adopt "passive" solutions like, for instance, having the DNA pass through nano-pores or nano-channels.

"Channels and filaments have physical features we may exploit to selectively let a type of molecule pass through" explains Micheletti. "You can have more or less entangled filaments and featuring knots of different types. In our study we have considered a specific DNA filament model and examined its behavior within a nano-channel. We have observed that by varying the channel's width it is possible to drastically change the quantity and complexity of the knots formed by the DNA."

The nano-channels may therefore be a tool with a double function: on one side they are used to understand the "knotting pattern" of a DNA fragment, on the other they may be used to select entangled filaments in the desired manner. The sectors employing DNA, mainly in sequencing, require an increasing number of new techniques to select the DNA filaments according to their characteristics, such as length, shape as well as entanglement.

More in detail...

"Experimental physicists will be, in the first instance, interested is such technique to obtain knot-free DNA", explains Micheletti referring to the usefulness of the methodology (that for now has been studied through simulation). "We should not forget that such method may also help us better understand, for instance, the functioning of topoisomerases, enzymes that have a very important role in cell metabolism."

Such enzymes play a key role in an organism: they maintain the DNA stretched out when the cell is not undergoing the cell division process.

"We are used to envisage chromosomes in their typical rod shaped appearance, the one preceding mitosis, that is to say cell reproduction," adds Micheletti. "However, usually the DNA is a sort of indistinct bundle that fills up the cell's nucleus. The topoisomerases maintain the disentangled filaments with the lowest possible rate of knotting, and do so by snipping and reattaching the little pieces of genetic material." Only on the "disentangled" filament all those transcription processes which are fundamental to the survival of an organism can actually function.

"The functioning of such enzymes may be better grasped if, before having them perform, we already know to what extent the molecule was entangled in the first place, and our methodology may be useful to this purpose." concludes Micheletti.


'/>"/>

Contact: Federica Sgorbissa
pressroom@sissa.it
39-040-378-7557
International School of Advanced Studies (SISSA)
Source:Eurekalert  

Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
The nano-channel that disentangles knots
(Date:3/11/2016)... -- http://www.apimages.com ) - --> http://www.apimages.com ) ... AP Images ( http://www.apimages.com ) - Germany ... the new refugee identity cards. DERMALOG will be unveiling this device, ... Hanover next week.   --> Germany ... produce the new refugee identity cards. DERMALOG will be unveiling this ...
(Date:3/9/2016)... , March 9, 2016 This BCC ... future states of the RNA Sequencing (RNA Seq) market ... such as instruments, tools and reagents, data analysis, and ... various segments of the RNA-Sequencing market such as RNA-Sequencing ... Identify the main factors affecting each segment and forecast ...
(Date:3/3/2016)... 2016  FlexTech, a SEMI Strategic Association Partner, awarded ... & Development, Leadership in Education, and, in a category ... th year of the FLEXI Awards and the ... from past years . Judging was done on ... of criteria, by a panel of non-affiliated, independent, industry ...
Breaking Biology News(10 mins):
(Date:5/5/2016)... ... May 05, 2016 , ... American ... two additional patents, U.S. Patent Nos. 9,322,133 and 9,322,134, to API and its ... nanocellulose as well as hydrophobic nanocellulose compositions. In addition to these patents ...
(Date:5/5/2016)... BELL, Pa. , May 5, 2016  Why ... Powers and Lorna Weir launching a ... un-agency way? Because they believe that truly helping clients ... world of healthcare now demands a different type of ... strategy lab serving the pharmaceutical, biotechnology and medical device ...
(Date:5/5/2016)... , ... May 05, 2016 , ... ... previously announced identification of its first three targets, it has identified a fourth ... beta (Aß), implicated in the development and progression of Alzheimer’s disease (AD). ...
(Date:5/4/2016)... ... 2016 , ... CereScan, the nation’s leader in providing statistically ... Stroke Awareness Month in May. An infographic created by CereScan will be ... will donate $1 up to a maximum of $3,000 through users who share ...
Breaking Biology Technology: