Navigation Links
The developmental dynamics of the maize leaf transcriptome
Date:11/3/2010

Photosynthesis is arguably the most impressive feat of nature, where plants harvest light energy and convert it into the building blocks of life at fantastically high efficiency. Indeed modern civilization became possible only with the cultivation of plants for food, shelter and clothing.

While scientists have been able to discover details of the fascinating process by which plants store solar energy as chemical energy, how developing plants build and regulate their solar reactors is still poorly understood. How many genes are involved, and which are the most important? How are different cell types endowed with specific biochemical capacities? What signals fine-tune how much sugar is produced, and which bioproducts are generated? The answers to these questions have applications in agriculture, bioenergy and climate change.

Complex and multifaceted questions such as these can be addressed using a new approach to measure gene expression using high throughput sequencing. This method, coined RNAseq, is detailed in a new report from scientists at the Boyce Thompson Institute for Plant Research (BTI) and Cornell University published online in the journal Nature Genetics. The study, using the agronomically critical maize (corn) plant as a model, tracks through massive sequencing of gene transcripts, the full complement of expressed genes in a corn leaf. The researchers found that as the leaf develops, entire suites of genes are turned on and off. "Previous studies have often focused on understanding one gene or set of genes that underpin a specific pathway or process", notes Tom Brutnell, an associate scientist at the BTI and the senior author of the study, "However, these new tools have let us examine the expression of all genes in the leaf at very specific stages of development. This provides an unprecedented view of the genetic circuitry of the leaf."

While such results are exciting, they also pose big challenges as scientists work to interpret the datasets. In this study, over 25,000 genes were found to be expressed in each leaf, and nearly half of these are transcribed into at least two different forms, called splicing variants. To make sense out of this flood of information, scientists at BTI, Cornell, Yale, Iowa State and the University of Toronto collaborated to develop systems biology tools that combine computational and statistical methods to analyze large datasets. "The interface between developmental biologists, molecular biologists, and computer scientists that made this work possible, is an excellent example of why systems biology is able to unravel complex biological pathways" commented David Stern, President of BTI.

An important aspect of this work is that it provides insight into the regulation of the unusual form of photosynthesis that maize utilizes referred to as C4 that increases water and nitrogen use efficiencies under hot dry environments. "Some of the most productive food, feed and bioenergy crops utilize C4 photosynthesis including maize, sugarcane and the bioenergy grass Miscanthus", notes Brutnell "This study provides the first comprehensive analysis of gene expression in any C4 plant and thus provides the groundwork for a genetic dissection of this process".

Given the economic importance of C4 plant in the United States and around the world, these findings have numerous potential applications to agriculture. Optimizing photosynthetic capacity could not only enhance the performance of maize, but also enable far-reaching transformations of photosynthesis in species which are currently far less efficient than maize, such as rice or wheat.


'/>"/>

Contact: Thomas Brutnell
TPB8@cornell.edu
Boyce Thompson Institute for Plant Research
Source:Eurekalert

Related biology news :

1. Digital zebrafish embryo provides the first complete developmental blueprint of a vertebrate
2. Carnegies Donald Brown receives lifetime achievement award from Society for Developmental Biology
3. Mouse model provides a new tool for investigators of human developmental disorder
4. Specific genetic cause of fetal alcohol-related developmental disorders found
5. Genetic marker linked to problem behaviors in adults with developmental disabilities
6. Developmental delay could stem from nicotinic receptor deletion
7. Chinese scientists discover marker indicating the developmental potential of stem cells
8. Tufts wins NCRR grant for Collaborative Cluster in Genome Structure and Developmental Patterning
9. Developmental problems: Some exist in the genes
10. Fertilizer chemicals linked to animal developmental woes
11. Developmental gene-environment interactions: A model for psychosis
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/20/2016)... , June 20, 2016 Securus Technologies, ... technology solutions for public safety, investigation, corrections and ... prisons involved, it has secured the final acceptance ... facilities for Managed Access Systems (MAS) installed. Furthermore, ... facilities to be installed by October, 2016. MAS ...
(Date:6/7/2016)...  Syngrafii Inc. and San Antonio Credit Union ... integrating Syngrafii,s patented LongPen™ eSignature "Wet" solution into ... result in greater convenience for SACU members and ... existing document workflow and compliance requirements. ... Highlights: ...
(Date:6/1/2016)... 2016 Favorable Government Initiatives Coupled ... Criminal Identification to Boost Global Biometrics System Market Through ... Research report, " Global Biometrics Market By Type, ... Opportunities, 2011 - 2021", the global biometrics market is ... account of growing security concerns across various end use ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... 2016 , ... Velocity Products, a division of Morris Group, ... exclusively for Okuma CNC machining centers at The International Manufacturing Technology Show, IMTS, ... companies with expertise in toolholding, cutting tools, machining dynamics and distribution, Velocity SMART ...
(Date:6/22/2016)... 2016 Research and Markets has announced the ... to their offering. The ... $29.3 billion in 2013. The market is expected to grow at ... to 2020, increasing from $50.6 billion in 2015 to $96.6 billion ... the forecast period (2015 to 2020) are discussed. As well, new ...
(Date:6/22/2016)...   ViaCyte, Inc. , a privately-held regenerative medicine ... therapy for the treatment of diabetes in clinical-stage development, ... ISSCR 2016, the Global Stem Cell Event, is taking ... San Francisco.    ... , Focus Session: Tools for Basic and Applied Stem ...
(Date:6/22/2016)... and ALBANY, N.Y. , ... (Teewinot) and Albany Molecular Research, Inc. (NASDAQ: ... licensed Teewinot,s technology to produce and sell the ... The CBCA analytical standard is manufactured using Teewinot,s ... expression of cannabinoid biosynthetic genes in microorganisms for ...
Breaking Biology Technology: