Navigation Links
The battle of the morphogens: How to get ahead in the nervous system
Date:9/1/2011

ic screen to search for Wnt inhibitors switched on by Vax. They found that Vax bound to a DNA sequence, or promoter, unusually positioned in the middle of a gene, rather than flanking it. And that gene---- designated Tcf7l2 ---- encoded a transcription factor normally deployed by Wnt to dorsalize target tissue.

The paradox was explained when the group showed that Vax activated expression of a molecular decoy, namely a stump of Tcf7l2 protein missing its front end, the part required for it to activate gene expression. When bound to DNA the fragment would instead recruit repressor proteins to silence dorsalizing signals. "Cells expressing the inhibitor would be blind to Wnt signaling," says Lemke.

Full-length Tcf7l2 proteins pair with an activator called -catenin. But truncated Tcf7l2 lacks the -catenin interaction region, short-circuiting its function. Scientists call such interfering proteins "dominant negatives."

Tomas Vacik, Ph.D., a postdoctoral fellow in the Lemke lab and the study's first author, re-evaluated gene expression patterns in mice the lab had engineered to lack Vax2. "We found that Vax2 was necessary for expression of a group of Wnt antagonists in the mouse eye, including dominant negative Tcf7l2" he says.

Bioinformatics analysis of the Tcf7l2 DNA sequence bound by Vax revealed another surprise. Approximately 700 base pairs, or nucleotides, of the mouse genome around the Vax binding site in Tcf7l2 showed an astonishing 99% identity between mouse, humans, and chickens and 85% identity with fish, a conservation Lemke calls, "exceptional in the extreme."

"This means that over several million years of evolution, Mother Nature says you can't change a single nucleotide, " he says. "That tells you straightway that this DNA sequence fulfills a very important regulatory function."

Database searches revealed that truncated Tcf7l2 is expressed in human brain cells, and the group's own analysis revealed sim
'/>"/>

Contact: Andy Hoang
Ahoang@salk.edu
619-861-5811
Salk Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Trudeau Institute announces new discovery in battle against infections
2. Battle won against Asian tiger mosquito
3. Trudeau Institute announces new discovery in battle against plague and bacterial pneumonias
4. Plant pathologist finding Kansas wheat fields a molecular battleground this season
5. Stevens biomedical engineering students fight hypothermia on the battlefield
6. Battle scars found on an ancient sea monster
7. New vitamin D-fortified food could battle widespread need for the sunshine vitamin
8. Whats coming next in the biochemical battle of the bulge?
9. Insect scientists battle invasive species in Hawaii
10. $40 million helps Cornell lead global battle against wheat pathogen that threatens food security
11. NIH funds center at Arizona State to battle infectious diseases
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
The battle of the morphogens: How to get ahead in the nervous system
(Date:7/9/2015)... July 9, 2015  Synaptics Inc. (NASDAQ: SYNA ), ... that it will report financial results for the fourth ... 2015 after the close of market. The company will ... at 2:00 p.m. PT (5:00 p.m. ET), during which ... participate on the live call, analysts and investors should ...
(Date:7/8/2015)... NEW YORK , July 8, 2015 ... cancer globally, and the fourth most fatal, with a ... year. The poor prognosis of pancreatic cancer patients has ... to treatment, which is not being met by the ... contains an array of products with varying molecule types ...
(Date:7/7/2015)... June 30, 2015 ... the addition of the "Capacitive Fingerprint Sensors ... To this date, fingerprint sensing technology is ... fingerprint sensors are well developed. This patent landscape ... The domain of capacitive fingerprint sensors ...
Breaking Biology News(10 mins):Synaptics to Report Fourth Quarter, Fiscal Year 2015 Results on July 30 2Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 2Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 3Worldwide Capacitive Fingerprint Sensors Patent Landscape Market Report 2015-2020 - Main IP Holders include Seiko Epson, AuthenTec, Sony and STMicroelectronics 2
... Veterinary researchers at the Virginia-Maryland Regional College of Veterinary ... the origin and possible evolution of an emerging swine virus ... least 17 states. A team of researchers led by ... used virus strains isolated from the ongoing outbreaks in Minnesota ...
... Famous as the sacred beetles of ancient Egypt the scarab ... globe. Some of the most vulnerable representatives are contained in ... known species. A recent study published in the open access ... with unusual distribution from southern Africa. The two new ...
... many years two companies located in Bizkaia, Bilbao Chemicals ... lindane and dumping it into the environment with no ... need to solve the problems caused by this dumping ... no viable process that will safely destroy the lindane ...
Cached Biology News:Veterinary scientists track the origin of a deadly emerging pig virus in the United States 2Veterinary scientists track the origin of a deadly emerging pig virus in the United States 3A fresh solution for the lindane problem 2A fresh solution for the lindane problem 3
(Date:7/31/2015)... 31, 2015 In today,s fast changing ... them drive positive health outcomes, improve profitability and ... patient care in their communities. At ThoughtSpot 2015, ... conference, AmerisourceBergen announced a new set of innovative ... Organization and help independent pharmacies endure the industry,s ...
(Date:7/30/2015)... -- AACC welcomed thousands of medical professionals and ... & Clinical Lab Expo in Atlanta ... revolutionary advancements in clinical testing technology and research ... diagnose patients quickly and accurately and make sure ... As of Wednesday, July 29, more than 17,500 ...
(Date:7/30/2015)... ... July 30, 2015 , ... ... field clinical study of its canine osteoarthritis stem cell product, currently under development ... City, KS) and will be marketed in the US by Aratana. This product, ...
(Date:7/30/2015)... -- HIGHLIGHTS:Q2 2015 Results (all changes are ... sales were $697 million compared to $701 million in ... 8%, and changes in foreign currency exchange rates decreased ... , By business unit, organic sales growth was ... SAFC Commercial. , Reported diluted EPS was $0.98 ...
Breaking Biology Technology:AmerisourceBergen Unveils Suite of Enhanced Capabilities for Independent Pharmacies at ThoughtSpot 2015 2AmerisourceBergen Unveils Suite of Enhanced Capabilities for Independent Pharmacies at ThoughtSpot 2015 3Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 2Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 3Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 4VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 2VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 3Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 2Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 3Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 4Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 5Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 6Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 7Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 8Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 9Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 10Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 11Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 12Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 13Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 14Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 15Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 16Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 17Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 18Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 19Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 20
... applications from microelectronics to power lines because of their ... stop those electrons in their tracks? Rice physicist ... Aharonov-Bohm effect -- the interaction between electrically charged particles ... carbon nanotubes. While doing so, they came to ...
... DUBLIN and PHILADELPHIA , ... SHPGY ), the global specialty biopharmaceutical company,announces that its ... a lawsuit in the U.S. District,Court for the District of ... Cadila and Zydus Pharmaceuticals ( USA ), Inc. (collectively,"Zydus") for ...
... to drop a toothpick on the head of one particular ... impossible, yet this degree of precision at the cellular level ... University Institute for NanoBioTechnology. Their study was published online in ... team used precise electrical fields as "tweezers" to guide and ...
Cached Biology Technology:Magnets trump metallics 2Lialda(R) - Shire Files Suit Against Cadila Healthcare Limited, Doing Business as Zydus Cadila and Zydus Pharmaceuticals (USA), Inc. 2Lialda(R) - Shire Files Suit Against Cadila Healthcare Limited, Doing Business as Zydus Cadila and Zydus Pharmaceuticals (USA), Inc. 3Pinpoint precision: Delivering a biochemical payload to 1 cell 2Pinpoint precision: Delivering a biochemical payload to 1 cell 3
These macrocarriers are for the biolistic PDS-1000 He and Hepta systems. Supplied as 500 macrocarriers....
Anti Human S14G-Humanin Polyclonal Antibody...
...
... Science Major Science MS 300V (Cat. No. ... designed to meet most electrophoresis needs in ... Major Science MS 300V power supply ... instruments used in electrophoresis. 4 pairs of ...
Biology Products: