Navigation Links
The activity of a bacterial effector protein seen in molecular detail
Date:6/11/2012

Many plant and animal pathogens deploy effector proteins as part of their 'molecular arsenal' to facilitate infection and colonisation of their hosts. New research has revealed the structure of a bacterial effector molecule bound to its target protein in the host.

Gram-negative bacterial pathogens can deliver effector proteins through a specific secretion system, directly into host cells, to manipulate host cell processes for the benefit of the pathogen. The host cell processes targeted by effectors and the mechanisms used for manipulation are diverse. Knowledge of how effectors interface with host cell molecules is critical for understanding both mechanisms of pathogenesis and how effectors could be used to deliver new insights into host cell biology.

Dr Mark Banfield at the John Innes Centre, funded by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC), has uncovered the structure of a bacterial effector molecule called 'Cif' bound to its target (NEDD8) from the host. The Cif effector is found in a number of pathogenic bacteria including strains of E. coli, Burkholderia, Photorhabdus and Yersinia species.

Delivery of Cif into host cells results in perturbation of the cell division cycle. One hypothesis suggests this could prevent rapid renewal of infected cells such as those in the lining of the gut, so helping bacterial colonisation. Researchers at the John Innes Centre also studied the enzymatic activity of the effector protein in solution, and in collaboration with Drs. Taieb and Oswald, who are based in Toulouse, France, in model host cells.

Their work, publishing in the Proceedings of the National Academy of Sciences Online Early Edition this week, used protein structure determination to reveal the interface formed between the bacterial effector and the host target. The knowledge of this interface at the molecular level allowed small, directed changes to be made to the effector to determine the regions of the interface important for the interaction and the enzymatic activity in solution and in cells.

A thorough understanding of how this effector acts at the molecular level not only provides new information about the virulence mechanisms used by pathogens, it also suggests ways in which these effectors could be used as tools to probe functions related to the cell cycle, and how this relates to cellular biology.


'/>"/>

Contact: Andrew Chapple
andrew.chapple@nbi.ac.uk
44-160-325-1490
Norwich BioScience Institutes
Source:Eurekalert

Related biology news :

1. NIH-funded study examines use of mobile technology to improve diet and physical activity behavior
2. NASAs new satellite movie of 1 weeks ash activity from Mexicos Popocatepetl Volcano
3. High levels of TRAIL protein in breast milk might contribute to anticancer activity
4. Activity in brain networks related to features of depression
5. Researchers develop rapid test strips for bacterial contamination in swimming water
6. Bacterial shock to recapture essential phosphate
7. Identification of differential proteins in maternal serum with Down syndrome
8. Speeding up drug discovery with rapid 3-D mapping of proteins
9. CSHL researchers solve structure of human protein critical for silencing genes
10. Copy of the genetic makeup travels in a protein suitcase
11. A non-invasive intracellular thermometer with fluorescent proteins has been created
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/2/2016)... 2, 2016  BioMEMS devices deployed in ... on medical screening and diagnostic applications, such ... devices that facilitate and assure continuous monitoring ... are being bolstered through new opportunities offered ... acquisition coupled with wireless connectivity and low ...
(Date:2/2/2016)... 2016   Parabon NanoLabs (Parabon) announced ... Research Office and the Defense Forensics and Biometrics ... the company,s Snapshot Kinship Inference software ... generally, defense-related DNA forensics.  Although Snapshot is best ... and ancestry from DNA evidence), it also has ...
(Date:2/1/2016)... Feb. 1, 2016  Wocket® smart wallet ( www.wocketwallet.com ) announces the ... Joey Fatone . Las Vegas , where ... --> Las Vegas , where Joey appeared at ... The new video ad was filmed at the Consumer Electronics Show (CES2016) ... Wocket booth to meet and greet fans. --> ...
Breaking Biology News(10 mins):
(Date:2/10/2016)... 2016 NX Prenatal Inc., a US ... technology for early warning of adverse pregnancy outcomes, ... study by Dr. Thomas McElrath of ... Fetal Medicine,s (SMFM) annual meeting held in ... 2016.  The presentation reported initial positive top-line results ...
(Date:2/10/2016)... Wash., Feb. 10, 2016  IsoRay, Inc. (NYSE MKT: ... brachytherapy and medical radioisotope applications for the treatment of ... today announced its financial results for the second quarter ... 31, 2015. --> ... quarter of fiscal 2016, which ended December 31, 2015, ...
(Date:2/10/2016)... ... February 10, 2016 , ... Benchmark Research, ... the promotion of two long-standing principal investigators (PI) to the roles of Chief ... and Development. , Dr. Laurence Chu, a Benchmark Research PI in the Austin ...
(Date:2/10/2016)... , ... February 10, 2016 , ... ... to their comprehensive training and support program, Sonalinkā„¢ remote monitoring. The inaugural launch ... performed on Friday, February 5th, connecting Dr. Samuel Peretsman to a HIFU technical ...
Breaking Biology Technology: