CHAMPAIGN, Ill. A new statistical technique developed by researchers at the University of Illinois allows scientists to scan a genome for specific gene-regulatory regions without requiring prior knowledge of the relevant transcription factors. The technique has been experimentally validated in both the mouse genome and the fruit fly genome.
"Our statistical approach is more widely applicable than other approaches, and is especially useful for investigating gene regulatory networks with relatively little prior characterization," said Saurabh Sinha, a professor of computer science and an affiliate of the university's Institute for Genomic Biology.
Sinha and colleagues at the U. of I., the University of Cambridge and the State University of New York described the technique and reported their findings in the Oct. 20 issue of the journal Developmental Cell. Sinha is a corresponding author of the paper.
Proper gene regulation depends upon certain combinations of molecules being attached to segments of DNA at the right place and at the right time. Such combinatorial regulation is arguably the most prominent theme in animal development, Sinha said.
The clusters of binding sites to which the necessary molecules attach are called cis-regulatory modules. Finding the appropriate cis-regulatory modules is the first step toward unraveling the gene regulatory process.
Beginning with a short list of sample cis-regulatory module sequences (obtained through genetic assays) the computer algorithms search genome-wide for other sequences with similar-looking binding sites and similar functionality.
"Given a representative subset of cis-regulatory module sequences, we should be able to predict most of the other modules," Sinha said. "Ultimately, the task is to draw a complete picture of what molecules are sitting where, and what combination of molecules is controlling each gene."
The researchers performed extensive cr
'/>"/>
| Contact: James E. Kloeppel kloeppel@illinois.edu 217-244-1073 University of Illinois at Urbana-Champaign Source:Eurekalert |
Related biology news :
1. New neuroimaging analysis technique identifies impact of Alzheimers disease gene in healthy brains
2. Ancient penguin DNA raises doubts about accuracy of genetic dating techniques
3. UF scientists discover new explanation for controversial old patient-care technique
4. New nanochemistry technique encases single molecules in microdroplets
5. Breathing technique can reduce frequency, severity of asthma attacks
6. ORNL scientists hone technique to safeguard water supplies
7. Ben-Gurion U. developing new computer techniques to analyze historic Hebrew and Arabic documents
8. Scary ancient spiders revealed in 3-D models, thanks to new imaging technique
9. New advance in revolutionary bullet fingerprinting technique
10. New technique can fast-track better ionic liquids for biomass pre-treatments
11. New MRI technique could mean fewer breast biopsies in high-risk women
| Copyright © 2003-2012 Bio-Medicine. All rights reserved. ABOUT | CONTACT US | DISCLAIMER | PRIVACY POLICY | TERMS AND CONDITIONS |  |