Navigation Links
Team overcomes major obstacles to cellulosic biofuel production
Date:12/27/2010

er. Cellobiose, a part of plant cell walls, consists of two glucose sugars linked together. Cellobiose is traditionally converted to glucose outside the yeast cell before entering the cell through glucose transporters for conversion to ethanol. Having a cellobiose transporter means that the engineered yeast can bring cellobiose directly into the cell. Only after the cellobiose is inside the cell is it converted to glucose.

This approach, initially developed by co-corresponding author Jamie Cate at the Lawrence Berkeley National Laboratory and the University of California at Berkeley, eliminates the costly step of adding a cellobiose-degrading enzyme to the lignocellulose mixture before the yeast consumes it.

It has the added advantage of circumventing the yeast's own preference for glucose. Because the glucose can now "sneak" into the yeast in the form of cellobiose, the glucose transporters can focus on drawing xylose into the cell instead. Cate worked with Jonathan Galazka, of UC Berkeley, to clone the transporter and enzyme used in the new strain.

The team then tackled the problems associated with xylose metabolism. The researchers inserted three genes into S. cerevisiae from a xylose-consuming yeast, Picchia stipitis.

Graduate student Soo Rin Kim at the University of Illinois identified a bottleneck in this metabolic pathway, however. By adjusting the relative production of these enzymes, the researchers eliminated the bottleneck and boosted the speed and efficiency of xylose metabolism in the new strain.

They also engineered an artificial "isoenzyme" that balanced the proportion of two important cofactors so that the accumulation of xylitol, a byproduct in the xylose assimilitary pathway, could be minimized. Finally, the team used "evolutionary engineering" to optimize the new strain's ability to utilize xylose.

T
'/>"/>

Contact: Diana Yates
diya@illinois.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. New study examines immunity in emerging species of a major mosquito carrer of malaria
2. States now fund majority of human embryonic stem cell research
3. Montana State University paleontologist receives major award for protecting fossils on federal land
4. Worldwide press room opens for major acoustical science and technology conference, Nov. 15-19
5. Highlights from major acoustical science and technology conference, Nov. 15-19
6. UNHs Fred Short adds seagrass data to major conservation study
7. Major award to NOC marine geoscientist
8. Worlds largest, most complex marine virus is major player in ocean ecosystems: UBC research
9. Major acoustical science and technology conference: Nov. 15-19
10. Paraquat resistance discovered in major weed
11. Major component in turmeric enhance effect of chemotherapy drug in head and neck cancer
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Team overcomes major obstacles to cellulosic biofuel production
(Date:7/9/2014)... With over 100 diseases that can attack soybean crops, ... the most wanted list? University of Illinois scientists cite ... research is needed on the fungus that causes charcoal ... damp growing conditions but Macrophomina phaseolina , the ... drought conditions. , "As the climate continues to change ...
(Date:7/9/2014)... with pain and fever but often can be defeated with ... and hard to beat. Now, scientists have built a new ... pyramids. Published in the journal ACS Applied Materials & ... and kill more of them than medicine alone. , David ... can lie in wait, undetectable in the human body or ...
(Date:7/9/2014)... may be on the verge of an economy driven by ... than coal and is undergoing a production boom. It has ... recent research is casting serious doubts over just how climate-friendly ... Engineering News (C&EN), the weekly news magazine of the ... senior correspondent at C&EN, explains that when burned as a ...
Breaking Biology News(10 mins):Climate change provides good growing conditions for charcoal rot in soybeans 2Climate change provides good growing conditions for charcoal rot in soybeans 3
... have identified 23 new longevity genes by screening the ... used as a model organism in genetics studies. The ... Genetics. , Each of the 23 genes somehow normally ... them increases lifespan. For example, the worm's lifespan doubles ...
... Unlike most mammals, cats--both domestic and wild--are indifferent ... explains the molecular mechanism behind their strictly carnivorous ... it molecularly," says Joseph Brand, senior author of ... Chemical Senses Center. , Scattered on mammalian tongues ...
... invaluable decision-making tools for public health officials. As ... 2001, models can be useful in two ways: ... infection and they can allow the comparison of ... implicit assumptions that may systematically bias their predictions, ...
Cached Biology News:Field of beams - Novel system uses polarized light pulses to reveal crop health 2
(Date:1/15/2014)... Jacobs Automotive, a group of car dealerships headquartered in ... Blood Center and offering free oil change coupons for participation ... Jan. 18 at the Bill Jacobs Cadillac and Chevrolet location ... http://photos.prnewswire.com/prnh/20140115/MN46637 )  The Bill Jacobs Auto annual ...
(Date:1/15/2014)... January 15, 2014 Cynvenio, a cancer ... through the genomic analysis of tumor cells in the ... a web-based cancer decision support application powered by CollabRx, ... Cynvenio’s ClearID™ genomic test, Therapy Finder will provide breast ...
(Date:1/15/2014)... , January 15, 2014 A study ... races on the Formula 1 track could help to tackle ... Applied Technologies (MAT), Stowhealth (a GP surgery based in Stowmarket) ... healthcare provider Simplyhealth. Telemetry technology, which is inspired ...
(Date:1/15/2014)... NC (PRWEB) January 15, 2014 DTS ... to its Online Web Portal for Life Science organizations who ... to specify the subject matter of their documents in advance ... will help reduce time-to-delivery of translations, often a critical factor ...
Breaking Biology Technology:Join the Bill Jacobs Auto blood drive and receive a free oil change 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 2Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 4DTS Improves Efficiency for Life Science Document Translations 2
... Benefit From Hidden Scar Surgical Technique ... Performed Through the Belly Button, ... successful completion of what are believed to be the,first ever ... by Dr. Jeffrey Cadeddu, associate professor of,urology and radiology at ...
... Hotel in New York on Thursday, ... ... (Nasdaq:,FVRL), a biopharmaceutical company developing patient-specific, active,immunotherapies for the treatment of ... Officer, will present at,the NewsMakers in the Biotech Industry investment conference ...
... DNA sequencing equipment market,is expected to grow nearly ... million to $528 million as competition intensifies and ... a new report by,Kalorama Information. The DNA ... last,several years, with one dominant vendor and relatively ...
Cached Biology Technology:Novare Announces First Ever Single Port Laparoscopic Kidney Removal (Nephrectomy) Using RealHand(TM) HD Instruments 2Favrille CEO to Present at NewsMakers in the Biotech Industry Investment Conference 2DNA Sequencing Equipment Market to Fragment and Grow as Competition, New Technologies Develop 2
Conveniently document gels, films and membranes with a high quality thermal printer. , Compact, footprint printer , Supplies 256 gray scale for high resolution, archive-quality thermal film prints...
... ICE-LAP3 Mch3 Lyophilized solid. ... enzyme regulation and kinetics, cleavage of target substrates, and ... protein. One unit is defined as the ... Ac-DEVD-pNA Cat. No. 235400 per hour at 37 C ...
Request Info...
Bovine Coronary Artery Endothelial Cells (BCAEC) (>500,000 cells)...
Biology Products: