Navigation Links
Team overcomes major obstacles to cellulosic biofuel production
Date:12/27/2010

er. Cellobiose, a part of plant cell walls, consists of two glucose sugars linked together. Cellobiose is traditionally converted to glucose outside the yeast cell before entering the cell through glucose transporters for conversion to ethanol. Having a cellobiose transporter means that the engineered yeast can bring cellobiose directly into the cell. Only after the cellobiose is inside the cell is it converted to glucose.

This approach, initially developed by co-corresponding author Jamie Cate at the Lawrence Berkeley National Laboratory and the University of California at Berkeley, eliminates the costly step of adding a cellobiose-degrading enzyme to the lignocellulose mixture before the yeast consumes it.

It has the added advantage of circumventing the yeast's own preference for glucose. Because the glucose can now "sneak" into the yeast in the form of cellobiose, the glucose transporters can focus on drawing xylose into the cell instead. Cate worked with Jonathan Galazka, of UC Berkeley, to clone the transporter and enzyme used in the new strain.

The team then tackled the problems associated with xylose metabolism. The researchers inserted three genes into S. cerevisiae from a xylose-consuming yeast, Picchia stipitis.

Graduate student Soo Rin Kim at the University of Illinois identified a bottleneck in this metabolic pathway, however. By adjusting the relative production of these enzymes, the researchers eliminated the bottleneck and boosted the speed and efficiency of xylose metabolism in the new strain.

They also engineered an artificial "isoenzyme" that balanced the proportion of two important cofactors so that the accumulation of xylitol, a byproduct in the xylose assimilitary pathway, could be minimized. Finally, the team used "evolutionary engineering" to optimize the new strain's ability to utilize xylose.

T
'/>"/>

Contact: Diana Yates
diya@illinois.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. New study examines immunity in emerging species of a major mosquito carrer of malaria
2. States now fund majority of human embryonic stem cell research
3. Montana State University paleontologist receives major award for protecting fossils on federal land
4. Worldwide press room opens for major acoustical science and technology conference, Nov. 15-19
5. Highlights from major acoustical science and technology conference, Nov. 15-19
6. UNHs Fred Short adds seagrass data to major conservation study
7. Major award to NOC marine geoscientist
8. Worlds largest, most complex marine virus is major player in ocean ecosystems: UBC research
9. Major acoustical science and technology conference: Nov. 15-19
10. Paraquat resistance discovered in major weed
11. Major component in turmeric enhance effect of chemotherapy drug in head and neck cancer
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Team overcomes major obstacles to cellulosic biofuel production
(Date:10/15/2014)... 44th Rosenstiel Award for Distinguished Work in Biomedical Science ... mechanisms of genomic instability and its implications for the ... alumnus to win the Rosenstiel Award; the first, Rod ... is the Charles A. Janeway Professor of Pediatrics and ... investigator at the Howard Hughes Medical Institute at Boston ...
(Date:10/14/2014)... new study published in Cancer Research shows ... and colon cancers—can promote the development of skin cancers ... and survival of sun-damaged skin cells. , Previously considered ... proteins called sirtuins that help regulate genomic stability and ... SIRT6 helps repair DNA damage, which can lead to ...
(Date:10/14/2014)... questions is how to produce enough food to feed ... and Agriculture Organization of the United Nations predicts that ... years to feed a growing global population, and plants ... food production. Plants—grains, cereals, fruits, vegetables, and more—feed ... research must tap into our knowledge of how plants ...
Breaking Biology News(10 mins):Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2Two-faced gene: SIRT6 prevents some cancers but promotes sun-induced skin cancer 2Building a bridge from basic botany to applied agriculture 2Building a bridge from basic botany to applied agriculture 3
... Northern Arizona University and the National Center for Ecological ... circuit theory to predict gene flow across landscapes. Their ... the best spots for wildlife corridors, which are crucial ... similarities between circuit theory and ecological connectivity, said Brad ...
... and Post-It Notes Three things that started out as obscure ... will the next idea to stick with modern society look ... a humble lizard, according to a University of Calgary biologist ... to virtually any surface, including glass., Unlike most creatures, geckos ...
... University of the Basque Country (UPV/EHU), an in vitro culture ... reproduce the gene regulation that is expressed in these cancer ... patients. This in vitro culture model provides a simple tool ... factors regulating this expression, as well as a simple form ...
Cached Biology News:Model connects circuit theory to wildlife corridors 2Sticky questions tackled in gecko research 2It is important to demonstrate the influence of the microenvironment in the process of metastasis 2It is important to demonstrate the influence of the microenvironment in the process of metastasis 3
(Date:10/20/2014)... The report “Pharmacy Automation Systems ... and Labeling Systems, Table-top Counters) by End-user (Inpatient ... & Trends to 2019” analyzes and studies the ... America, Europe, Asia-Pacific, and the Rest of the ... 30 figures spread through 300 pages and in-depth ...
(Date:10/20/2014)... PARK, Calif. , Oct. 20, 2014 /PRNewswire/ ... today that the Company has signed a Notice ... of Regenerative Medicine (CIRM), effective October 1, 2014.  ... Clinical Development payments and the release of additional ... million CIRM grant award for clinical development of ...
(Date:10/20/2014)... 20, 2014  GenVec, Inc. (NASDAQ: GNVC ) ... Ph.D., from its board of directors effective on October 24, ... and served as its chairman from June 2006 to November ... and Corporate Governance and Audit Committees of the board.  ... decade of dedicated service to GenVec, and its stockholders," said ...
(Date:10/20/2014)... Earle Martin , Chief Executive ... Ellen Teplitzky, an experienced attorney specializing in business ... has joined the firm as Director of Legal ... NDA Partners provides legal services, including research, ... top law firms and their clients in cases ...
Breaking Biology Technology:Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 2Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 3Pharmacy Automation Systems Market worth $4,566.2 Million by 2019 - New Research Report by MarketsandMarkets 4Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4Zola P. Horovitz To Retire From GenVec Board 2NDA Partners Appoints Ellen Teplitzky, JD as Director of its Legal Services Practice 2
... Syngenta today announced that it has acquired Georgia-based ... protection technology to the company,s portfolio. Circle One Global ... a toxin that can develop in crops such as corn ... , "The acquisition of this technology adds an important ...
... ... Biotechnology Laboratory, and support US and European power supplies. , ... Williamstown, NJ (PRWEB) June 11, 2009 -- ... box. The CS230000 and CS230001 both are new Biotechnology kits from A-WIT Technologies. Choose ...
... ... financial services offerings to further meet the needs of emerging businesses. ... on Demand - NSS partners with their clients acting as an outsourced ... Solutions team equips its clients with multiple game plans; a blue print ...
Cached Biology Technology:Syngenta Acquires Innovative Bio-Control Technology to Improve Usable Crop Yields 2Syngenta Acquires Innovative Bio-Control Technology to Improve Usable Crop Yields 3A-WIT Technologies Releases World's First Low Cost C Stamp(TM) Based Biotechnology Laboratory in a Box 2Next Stage Solutions Announces Three New Financial Services For Emerging Business 2Next Stage Solutions Announces Three New Financial Services For Emerging Business 3
Sheep polyclonal to Quinolone ( Abpromise for all tested applications). Antigen: Norfloxacin(N)conjugated to BSA...
...
...
... the 9, 10, 12, and 13 positions. It ... for the quantification of 9(S)-HODE by GC- or ... of linoleic acid in both plants and animals. ... an esterified component of membrane phospholipids and in ...
Biology Products: