Navigation Links
TGen method isolates biospecimens for treatment of kidney disease
Date:7/11/2012

PHOENIX, Ariz. July 11, 2012 Researchers at the Translational Genomics Research Institute (TGen) have developed a method of isolating biospecimens that could lead to a less costly, less invasive and more accurate way of diagnosing chronic kidney disease, or CKD.

CKD is a major complication of diabetes, high blood pressure and a form of kidney disease known as glomerulonephritis, which is characterized by a progressive deterioration of the kidney's ability to filter waste from the blood.

TGen's customized procedure produced high amounts of protein-rich urinary exosomes, which are microscopic vesicles that could help scientists discover biomarkers leading to better diagnosis and treatment of CKD.

TGen's findings were published today in the journal Kidney International, part of the Nature Publishing Group.

"Our method of extracting exosomes from urine is simple, fast and easily adapted to clinical research, so we can ultimately help physicians provide better therapies for their patients," said Dr. Johanna DiStefano, Director of TGen's Diabetes, Cardiovascular and Metabolic Diseases Division, and the study's senior author.

The goal of the study was to identify the best exosome isolation methods for both proteomic analysis and RNA profiling of urinary exosomes as a first step for biomarker discovery.

"Unlike a kidney biopsy an invasive and expensive procedure that provides only a small sample from one of two kidneys urinary exosomes provide a full representation of the entire urinary system," said Dr. Lucrecia Alvarez, a post-doctoral fellow at TGen and the study's lead author.

TGen researchers evaluated six different methods of isolating exosomes, which can carry genetic material that, when profiled, can lead to biomarkers that could help identify patients with kidney disease.

One of the six methods in the study tested was based on a commercially available exosome precipitation reagent called ExoQuick-TC, which by itself did not yield high quantities or pure preparations of protein and RNA. However, the TGen modification of the protocol led to the highest yields of miRNA and mRNA, which can subsequently be used in downstream genetic profiling experiments.

CKD typically is diagnosed by detecting increased levels of urinary albumin, a protein that is filtered out of urine in healthy kidneys, or of serum creatinine, a breakdown product of creatine, which is an important part of muscle. Neither measurement, however, can accurately indicate the type of kidney injury or disease. Significant kidney disease can be present with minimal or even no change in levels of albumin or creatinine.

Biomarkers can serve as indicators of normal biological processes, disease development or response to therapeutic drugs.

"In contrast to renal biopsy, urine is an ideal source of biomarkers, particularly for diseases of the kidney and urinary tract because it can be conveniently collected in large amounts without risk to the patient," said Dr. DiStefano.

TGen's method of isolating exosomes in urine is an improvement over the use of today's mostly commonly used centrifuge processes. The current method is time-consuming, requires expensive equipment, can only process low volumes, and only a few samples of urine at a time, which makes it unsuitable for clinical use.

Another current method is ultrafiltration, which tends to retain unwanted proteins that interfere with the isolation of exosomal proteins.

"Faster and more efficient methods of isolating exosomes are needed," Dr. Alvarez said. "The method we developed provides strong potential for identifying and characterizing exosomal biomarkers from urine, which presents favorable implications for the diagnosis and treatment of chronic kidney disorders."


'/>"/>
Contact: Steve Yozwiak
syozwiak@tgen.org
602-343-8704
The Translational Genomics Research Institute
Source:Eurekalert

Related biology news :

1. No matter the drilling method, natural gas is a much-needed tool to battle global warming
2. Stealthy microscopy method visualizes E. coli sub-cellular structure in 3-D
3. Researchers develop new method for analyzing cell function
4. New method generates cardiac muscle patches from stem cells
5. New drug-screening method yields long-sought anti-HIV compounds
6. UTMB researchers create powerful new method to analyze genetic data
7. Healing Method from Australia Addresses the Human Genome
8. A new method detects traces of veterinary drugs in baby food
9. China poised to accept first-ever non-animal test method for cosmetics
10. Chemical engineers at UMass Amherst find high-yield method of making xylene from biomass
11. Scientists advance field of research with publication of newly validated method for analyzing flavanols in cocoa
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/23/2017)... 23, 2017 Research and Markets has announced ... & Trends - Industry Forecast to 2025" report to their ... The ... CAGR of around 8.8% over the next decade to reach approximately ... the market estimates and forecasts for all the given segments on ...
(Date:3/20/2017)... March 20, 2017 PMD Healthcare announces the ... and Wellness Management System (WMS), a remote, real-time lung ... 2010, PMD Healthcare is a Medical Device, Digital Health, ... dedicated to creating innovative solutions that empower people to ... intent focus, PMD developed the first ever personal spirometer, ...
(Date:3/7/2017)... England , March 7, 2017 Brandwatch , ... chosen by The Prince,s Trust to uncover insights to ... across The Trust. The UK,s leading youth charity will ... social campaign results and get a better understanding of the topics ... ...
Breaking Biology News(10 mins):
(Date:4/25/2017)... ... April 25, 2017 , ... ... supplier of Common Lisp (CL) development tools, and market leader for Semantic ... key performance enhancements now available within the most effective system for developing and ...
(Date:4/25/2017)... ... April 25, 2017 , ... Covalent Metrology has ... Services unit provides high-quality data to clients, both faster and cheaper than ... There are no price premiums, and customers are welcome to participate in the ...
(Date:4/21/2017)... ... 21, 2017 , ... The 2017 Colorado Manufacturing Awards celebrates ... nominees and well as the first-year award for 2017 Manufacturing Women of the ... hosted by CompanyWeek and Manufacturers Edge, among other sponsors. , The Colorado ...
(Date:4/21/2017)... ... ... The AMA is happy to announce that $48,000 in scholarships will be awarded ... created through funds donated by model aviation organizations and individuals, AMA members, and a ... Committee, which is made up of model aviation pilots and enthusiasts. The committee evaluates ...
Breaking Biology Technology: