Navigation Links
TGen investigators devise faster, cheaper way of analyzing the human genome
Date:9/14/2008

PHOENIX, Ariz. Sept. 14, 2008 Investigators at the Translational Genomics Research Institute (TGen) today announced a faster and less expensive way for scientists to find which genes might affect human health.

Using bar-codes, not unlike what shoppers find in grocery stores, TGen researchers found a way to index portions of the nearly 3-billion-base human genetic code, making it easier for scientists to zero in on the regions most likely to show variations in genetic traits.

The findings were published today in the online version of the journal Nature Methods. The study will be published in print in the journal's October edition.

Dr. David Craig, associate director of TGen's Neurogenomics Division, said the new method should cost only one-tenth, or less, of the current cost of sequencing genes commonly done to analyze Single Nucleotide Polymorphisms (SNPs), and in performing Genome-Wide Association (GWA) studies.

"Our goal is to find the genetic basis of disease,'' said Craig, the study's lead author. "It (the new method) provides us a way to immediately use next-generation sequencing technology for studying hundreds to thousands of individuals.''

John Pearson, the head of TGen's Bioinformatics Research Unit, said the new method would allow scientists worldwide to more easily tune their sequencing experiments, and conduct their experiments with greater speed.

"In many cases, rather than sequencing the whole genome for 10 people, researchers would rather sequence a dozen genes for 1,000 people,'' said Pearson, who contributed to the study.

TGen scientists adapted an exciting new technology known as "next generation sequencing'' to allow samples to be run and analyzed using 15 well-characterized indexes.

"Moving forward, TGen scientists are now attempting to merge this indexing approach with sequence-capture methods currently under development in their laboratories, which would likely further improve the cost savings and speed,'' said Dr. Matthew Huentelman, an investigator in TGen's Neurogenomics Division, who also contributed to the study.

Depending on assumptions made in an experiment, the desired coverage -- and as a consequence, the cost -- can vary substantially, the study said, depending on whether the objective is:

  • Discovering genetic variants for genotyping by a separate method such as custom SNP genotyping.

  • Conducting polymorphism discovery and variant calling within one sequencing experiment.

  • Exhaustively resequencing for all common and rare variants.

The new method of analyzing human genetics should enable scientists at TGen and elsewhere to push ahead with key scientific research needed to prevent, diagnosis and treat a variety of diseases and conditions.

"Although whole-genome sequencing may be the primary motivator for improvements in sequencing technology,'' the study said, "it is clear that next-generation technologies are immediately useful for focused, hypothesis-driven sequencing of linkage peaks, groupings of candidate genes or sequencing the entire known coding sequence of the human genome.''


'/>"/>

Contact: Steve Yozwiak
syozwiak@tgen.org
602-343-8704
The Translational Genomics Research Institute
Source:Eurekalert

Related biology news :

1. Damon Runyon Cancer Research Foundation awards prestigious fellowships to 17 top young investigators
2. 4 Stanford faculty named Howard Hughes Medical Institute Investigators
3. 2 University of Illinois researchers named HHMI investigators
4. BWF awards $7 million to infectious disease investigators
5. Bioengineers at University of Pennsylvania devise nanoscale system to measure cellular forces
6. UF researchers devise way to calculate rates of evolution
7. Stowers Proteomics Center devises method for assigning probabilities to human protein interactions
8. Cold Spring Harbor Laboratory scientists devise potential approach to treat spinal muscular atrophy
9. Researchers devise means to create blood by identifying earliest stem cells
10. The power of multiples: Connecting wind farms can make a more reliable - and cheaper - power source
11. Cheaper drugs now closer to realization with new DropArray technology
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/7/2017)... 7, 2017 Zimmer Biomet Holdings, Inc. (NYSE ... will present at the LEERINK Partners 6th Annual Global ... on Wednesday, February 15, 2017 at 10 a.m. Eastern ... presentation can be accessed at http://wsw.com/webcast/leerink28/zbh .  The ... via Zimmer Biomet,s Investor Relations website at http://investor.zimmerbiomet.com ...
(Date:2/3/2017)... A new independent identity strategy consultancy firm ... . Designed to fill a critical niche in technical ... partners Mark Crego and Janice Kephart ... identity expertise that span federal governments, the 9/11 Commission, ... combined expertise has a common theme born from a ...
(Date:2/1/2017)... 1, 2017 IDTechEx Research, a leading provider ... announces the availability of a new report, Sensors for Robotics: ... Continue Reading ... ... robots. Source: IDTechEx Report "Sensors for Robotics: Technologies, Markets and Forecasts ...
Breaking Biology News(10 mins):
(Date:2/23/2017)... ... 23, 2017 , ... BellBrook Labs is formalizing a significant ... of biochemical analyses critical for Lead Discovery. The company’s Lead Discovery Services ... including inhibitor potency and selectivity, mechanism of action, and inhibitor residence times on ...
(Date:2/23/2017)... , Feb. 23, 2017  Imanis Life Sciences ... line of oncolytic vaccinia viruses for virotherapy research. ... part of Genelux,s proprietary, vaccinia virus-based technology platform ... to enter into a partnership with Genelux to ... vaccinia viruses for use in research," said Dr. ...
(Date:2/23/2017)... ... , ... The Greater Gift Initiative, Inc , (GGI) a Winston-Salem, NC ... GGI's mission is to advance global health and highlight the greater good of clinical ... each clinical trial volunteer. The vision of GGI is to serve as a philanthropic ...
(Date:2/22/2017)... -- Aratana Therapeutics, Inc. (NASDAQ: PETX), a pet therapeutics company focused ... for companion animals, will host a live conference call on ... financial results from the fourth quarter and full year ended ... may access the audio webcast or use the ... ...
Breaking Biology Technology: