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TB outbreaks could be 'solved' by DNA tracking
Date:9/3/2012

Reconstructing the spread of killer diseases such as tuberculosis (TB) from person to person using DNA sequencing quickly identifies the origin and movement of pathogens. This approach is directly informing public health strategies to control infectious disease outbreaks, says a scientist speaking at the Society for General Microbiology's Autumn Conference at the University of Warwick.

A team from the British Columbia Centre for Disease Control in Vancouver, Canada used whole-genome sequencing to analyse the bacterial DNA in samples from 36 of 41 infected individuals in a TB outbreak. They were able to track the pathogen's movements through the community in British Columbia, including where it started and who infected whom. From this they could identify key persons, places, and behaviours that contributed to the spread of disease. They showed how the social structure of a community contributed to the rapid spread of TB and that a rise in crack cocaine use in the area may have triggered the outbreak.

Earlier epidemiological tools analysed some - but not all - of the DNA in infected samples. This gave too little information to accurately reconstruct an outbreak and scientists could only make informed guesses at how a pathogen spread through a population. Lead researcher Dr Jennifer Gardy, speaking at the conference said, "'Solving' an outbreak - identifying the source of the disease and the underlying patterns of transmission - is the proverbial holy grail of epidemiology. Using whole-genome sequencing we are now able to act like field naturalists and observe how pathogens behave out in the wild. We can see where outbreaks start and how they spread. This level of insight has never been seen before and it promises to change the way we do public heath outbreak investigations."

The group is currently applying the same reconstruction technique to a different TB outbreak in a different social setting. "We are discovering how important locati
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Contact: Laura Udakis
l.udakis@sgm.ac.uk
44-079-908-26696
Society for General Microbiology
Source:Eurekalert  

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