Navigation Links
Synthetic protein mimics structure, function of metalloprotein in nature
Date:12/10/2009

CHAMPAIGN, Ill. Scientists have designed a synthetic protein that is both a structural model and a functional model of a native protein, nitric-oxide reductase.

The designed protein "provides an excellent model system for studying nitric-oxide reductase, and for creating biocatalysts for biotechnological, environmental and pharmaceutical applications," said University of Illinois chemistry professor Yi Lu, who directed the work.

"Through rational design, we can better understand native proteins, and maybe make one that is more efficient, more stable or more functional," Lu said.

While considerable progress has been made in designing proteins that mimic the structure of native proteins, the goal of reproducing both the structure and the function of native proteins especially metal-containing proteins called metalloproteins has been elusive.

Lu's research group, including lead author Natasha Yeung, and collaborators at the University of Illinois and at Brookhaven National Laboratory, are among the first to design a protein that mimics both the structure and the function of a metalloprotein. The researchers described their work in the journal Nature, published online on Nov. 25.

Nitric-oxide reductase is a key enzyme in the nitrogen cycle that is critical for life. Nitric oxide plays a key role in cell signaling and host-pathogen responses. Therefore, study of nitric-oxide reductase is an important step toward understanding these physiological and pathological processes.

It has been difficult to study nitric-oxide reductase, however, as it is a membrane protein that is not water soluble.

To mimic the structure and function of nitric-oxide reductase, the researchers began with myoglobin, a small muscle protein. Although smaller than nitric-oxide reductase and water soluble, myoglobin can reproduce key features of the native system. Into this scaffold protein the researchers engineered a new iron binding site consisting of three histidines and one glutamate.

In addition to their structural roles, the histidines and glutamate in the active site may also provide the two protons required for nitric oxide reduction.

"The designed protein models both the structure and the function of nitric-oxide reductase, and offers additional insight that the active site glutamate is required for both iron binding and reduction activity," Lu said. "The designed protein also serves as an excellent model for further mechanistic studies of nitric-oxide reductase."


'/>"/>

Contact: James E. Kloeppel
kloeppel@illinois.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert

Related biology news :

1. Synthetic biology offers new opportunities for interdisciplinary collaboration
2. New synthetic molecules trigger immune response to HIV and prostate cancer
3. Sun or shade: Pecan leaves photosynthetic light response evaluated
4. Synthetic cells shed biological insights while delivering battery power
5. Understanding a cells split personality aids synthetic circuits
6. Nanotechnology and synthetic biology: What does the American public think?
7. Synthetic Biology Project receives 2 National Science Foundation grants
8. July 9-10 symposium on synthetic biology
9. Synthetic catalyst mimics natures hydrogen economy
10. Synthetic chemical offers solution for crops facing drought
11. Using combinatorial libraries to engineer genetic circuits advances synthetic biology
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/1/2016)... , Feb. 1, 2016  Today, the first day ... announced plans to develop a first of its kind ... of IBM Watson. In the first application of ... (NYSE: IBM ), and Welltok will create a ... assessments with cognitive analytics, delivered on Welltok,s health optimization ...
(Date:1/27/2016)... Ohio , Jan. 27, 2016  Rite Track, ... based in West Chester, Ohio ... award winning service staff, based in Austin, ... capacity and ability to provide modifications, installations and technical ... Dovalina , CEO of PLUS, commented, "PLUS has provided ...
(Date:1/22/2016)... , January 22, 2016 ... the addition of the  "Global Behavioral ... offering. --> http://www.researchandmarkets.com/research/4lmf2s/global_behavioral ) ... "Global Behavioral Biometric Market 2016-2020"  report ... Research and Markets ( http://www.researchandmarkets.com/research/4lmf2s/global_behavioral ) has ...
Breaking Biology News(10 mins):
(Date:2/4/2016)... , Feb. 4, 2016  Sangamo BioSciences, Inc. (NASDAQ: ... announced today that Edward Lanphier , Sangamo,s president ... the progress of Sangamo,s ZFP Therapeutic ® development ... at 2:40 pm ET on Thursday, February 11, 2016, ... Healthcare Conference. The conference is being held in ...
(Date:2/4/2016)... Feb. 4, 2016 Beike Biotechnology, the ... institutions attended a ceremony in late 2015 to mark ... therapy in 2016. --> ... Platform for Personalized Cell Therapy" was hosted by the ... Center, both subsidiaries of Beike Biotechnology Co., Ltd. ...
(Date:2/4/2016)... , February 4, 2016 ... a biotechnology acceleration company is pleased to provide the following ... --> Over the last 3 months we have ... securities purchase agreements exceeding $1,000,000. As a result, we have ... Mannin Research Inc. license agreement and expect that development to ...
(Date:2/4/2016)... , Feb. 4, 2016 Sinovac Biotech ... a leading provider of biopharmaceutical products in ... of its board of directors received on February 4, ... 2016, from a consortium comprised of PKU V-Ming ( ... Biomedicine Co., Ltd., CICC Qianhai Development ( Shenzhen ...
Breaking Biology Technology: