Navigation Links
Supercharged proteins enter biology's forbidden zone
Date:7/14/2010

Scientists are reporting discovery of a way to help proteins such as the new generation of protein-based drugs -- sometimes heralded as tomorrow's potential "miracle cures" -- get past the biochemical "Entrance Forbidden" barrier that keeps them from entering cells and doing their work. The new technique, described in the monthly journal, ACS Chemical Biology, represents a new use for an engineered form of green fluorescent protein, the topic of the 2008 Nobel Prize in Chemistry that is one of the most important research tools in biosciences.

In the report, David Liu and his colleagues describe the difficulty of delivering proteins from the outside of mammalian cells into their interiors. For that reason, nearly all of the blockbuster drugs that are proteins must do their work on the exterior of cells, activating receptors that send signals to the interior. This constraint greatly limits the scope of protein-based drugs. Liu's solution: Attaching proteins to molecules of "supercharged" green fluorescent protein, which they mutated to give it a very high positive charge. When the hybrid proteins bump into the surface of a cell, they get pulled inside by negatively charged molecules called proteoglycans.

Liu and his team tested the hybrid proteins on five types of cells, and found that they were extremely good at carrying their protein cargo inside. In fact, the supercharged protein was up to 100 times better at getting proteins into cells compared to other approaches. The delivered proteins were able to go to their target locations in the cell, such as the nucleus or cytoplasm, and perform their jobs. "To our knowledge, this is the first report of functional delivery of an enzyme into retinal cells in vivo," the researchers state.


'/>"/>

Contact: Michael Bernstein
m_bernstein@acs.org
202-872-6042
American Chemical Society
Source:Eurekalert  

0

GOOD

Page: 1

Related biology news :

1. Saliva proteins could help detection of oral cancer
2. New lab manual focuses on essential methods for purifying and characterizing proteins
3. Proteins in sperm unlock understanding of male infertility says new study
4. Linking Proteins, Wires, Dots, and Molecules into Useful Devices
5. Luminescence shines new light on proteins
6. Proteins strangle cell during division
7. Just a little squeeze lets proteins assess DNA
8. New technique is quantum leap forward in understanding proteins
9. Motor nerve targeting to limb muscles is controlled by ephrin proteins
10. JDRF-funded researchers discover proteins regulating human beta cell replication
11. Fluorescent proteins illuminating biomedical research
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Supercharged proteins enter biology's forbidden zone
(Date:5/21/2013)... , May 21, 2013 Royal Philips Electronics ... the world,s first portable ultrasound with Philips, industry leading ... capability. The CX50 xMATRIX with available Live 3D TEE ... at the EuroPCR, official meeting for the ... Since its launch in 2012, Philips CX50 ...
(Date:5/20/2013)... also commonly resistant to antimicrobial substances made by ... mBio , the online open-access journal of ... and host antimicrobials LL-37 and lysozyme, which help ... that patients with life-threatening multi-drug resistant infections are ... is a last-line drug for treating several kinds ...
(Date:5/20/2013)... the effect of physical education (PE) on child weight, ... increasing the amount of time that elementary schoolchildren spent ... The study represents some of the first evidence of ... is forthcoming in the Journal of Health Economics ... can be viewed at: http://www.sciencedirect.com/science/article/pii/S0167629613000556 , The ...
Breaking Biology News(10 mins):Philips CX50 xMATRIX now offers world-class interventional and diagnostic features on single portable system 2Resistance to last-line antibiotic makes bacteria resistant to immune system 2Resistance to last-line antibiotic makes bacteria resistant to immune system 3Gym class reduces probability of obesity, study finds for first time 2
... planning of the preparatory activities for a new tentative ... satellite. On 7 September, preparatory activities for the ... Russia were stopped by Eurockot due to an anomaly ... and navigation subsystem of the launcher,s Upper Stage Breeze ...
... a highly selective behavior towards odor stimuli. A ... olfactory stimulus is often not sufficient for immediate ... Interestingly, the behavior differed between investigated D. ... mangos) were preferred by most flies, whereas artificial ...
... 1 OCTOBER 2008 Not only are more than ... Brown Convention Center, beginning this weekend, to participate in ... but they are bringing focus to issues of local ... theme of "Celebrating the International Year of Planet Earth," ...
Cached Biology News:What is wild? Odor attraction among different wildtype Drosophila 2What is wild? Odor attraction among different wildtype Drosophila 3Earth scientists keep an eye on Texas 2Earth scientists keep an eye on Texas 3Earth scientists keep an eye on Texas 4Earth scientists keep an eye on Texas 5
(Date:5/22/2013)... , May 22, 2013  Superior Controls, ... Year 2013" by Business NH Magazine.  For the ... State organizations that distinguish themselves with extraordinary business ... proud to receive this honor," said ...  "As with any recognition, this award is a ...
(Date:5/22/2013)... Mold remediation could take a toll on ... a way to cut down the process' expense. And so, ... the details of a method how to kill mold ... be prevented, My Cleaning Products said. But in case mold ... mold remediation must then be promptly implemented as it poses ...
(Date:5/22/2013)... Canada (PRWEB) May 22, 2013 ... approaches for deciphering the information they encode. There ... ribosome profiling (deep sequencing of ribosome protected fragments) ... in vivo. , Keynote speakers include Dr. Jonathan ... Francisco and the Howard Hughes Medical Institute, who ...
(Date:5/21/2013)... MA (PRWEB) May 21, 2013 ... tracking to reporting eLearning solution using Tin Can ... and dashboard tool. CLD is a leading developer ... and medical device companies worldwide. , Tin ... standard that helps learners and trainers more accurately ...
Breaking Biology Technology:Superior Controls of Seabrook, NH named Business of the Year for 2013 by Business NH Magazine 2How to Kill Mold While Saving Thousands of Dollars on Mold Remediation Projects Explained by My Cleaning Products 2Monitoring Protein Synthesis One Codon at a Time Through Ribosome Profiling, a Webinar Hosted by Xtalks 2CLD Integrates Tin CAN API with its eLearning Solutions and Launches its Excelerometer Tracking Statement Dashboard 2CLD Integrates Tin CAN API with its eLearning Solutions and Launches its Excelerometer Tracking Statement Dashboard 3
... Remedy Informatics, the leading provider of registries for Life ... Providence Health & Services, has selected Remedy,s Mosaic(TM) ... Telestroke Network to improve patient outcomes . ... excited to work with Providence, and to support their ...
... (NASDAQ: CPHD ) today announced that, together ... Jersey (UMDNJ), it has received a grant from the ... amplification methods for highly-sensitive detection of bloodstream bacteria using ... The $1.3 million grant is for the first year ...
... BOSTON, Massachusetts, March 20, 2012 Heptares ... an operating company of Shire PLC (LSE: SHP, NASDAQ: ... has exercised its exclusive option to license a novel ... is currently in preclinical development. Adenosine A 2A ...
Cached Biology Technology:Providence Health & Services Inks Deal with Remedy Informatics for Orthopedic and Stroke Registries 2Providence Health & Services Inks Deal with Remedy Informatics for Orthopedic and Stroke Registries 3Cepheid Receives Grant to Develop Sample Processing and Amplification Methods for Detection of Bloodstream Bacteria 2Cepheid Receives Grant to Develop Sample Processing and Amplification Methods for Detection of Bloodstream Bacteria 3Cepheid Receives Grant to Develop Sample Processing and Amplification Methods for Detection of Bloodstream Bacteria 4Heptares Grants Shire an Exclusive Worldwide Licence to Develop and Commercialise Novel Drug Candidate for CNS Disorders 2Heptares Grants Shire an Exclusive Worldwide Licence to Develop and Commercialise Novel Drug Candidate for CNS Disorders 3
... ImageXpressULTRA imaging system from Molecular ... confocal system for automated acquisition and ... cell-based screening. ImageXpressULTRA is powered ... for screening acquisition, image storage and ...
... Assay is an anchorage-independent growth assay in ... stringent assay for detecting malignant transformation of ... with carcinogens or carcinogen inhibitors) are cultured ... for 21-28 days. Following this incubation ...
... Imager delivers high resolution CCD confocal imaging ... effective optical package that fits on your ... combined with high quantum efficiency CCD cameras, ... recording at up to 100 fps. A ...
... blotting of E-PAGE Gels easier and more ... pad and several types of pre-cut pre-assembled ... cm x 13.5 cm E-PAGE Gels*. The ... designed for western transfer of proteins (Figure ...
Biology Products: