Navigation Links
Sulfurous ping-pong in the urinary tract
Date:12/18/2008

were analyzed at the Swiss Light Source at Paul Scherrer Institut in Villigen, Switzerland.

Two propellers building a cage

This analysis, down to 2 ngstrm resolution, revealed that ASST indeed contains an extremely short disulfide bond which can presumably only be formed by the action of the disulfide bond formation machinery genetically associated with ASST. This disulfide bridge is a prerequisite for proper folding of this protein and could also play a role in regulating its catalytic activity.

However, these features were almost outweighed by other unusual discoveries: the researchers found a previously never-observed protein structure to catalyze this process. This structure consists of two equal propeller-like parts which contain active sites in the center of the two propellers, built of beta-pleated sheets. Such a structure has never been observed for a sulfotransferase.

How does this two-propeller machine function? To answer this question, the scientists replaced individual amino acids, i.e. building blocks of the protein. In addition, they used molecules acting as sulfuryl-donors and repeated crystallographic analyses. Now they saw that five amino acids containing nitrogen are essential for the function of ASST. They built a reaction cage that accommodates both the donor and the acceptor of the sulfuryl group. Furthermore, during the transfer, the sulfuryl group is directly, covalently bound to a histidine side chain of ASST. Thus, the signal is first transferred from the donor to ASST and subsequently from ASST to the acceptor. Such a ping-pong mechanism is unique in the processes of sulfuryl transfer.

Point of vantage against the bad" E. coli strains

A new structure, a new mechanism this opens up medically relevant perspectives. Goran Malojčić; the first author of this study, explains several interesting points. Since ASST is not present in mammals, the protein could be
'/>"/>

Contact: Prof. Rudi Glockshuber
rudi@mol.biol.ethz.ch
41-446-332-351
ETH Zurich/Swiss Federal Institute of Technology
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Bacteria that cause urinary tract infections invade bladder cells
2. Cell transplants may improve severe urinary incontinence
3. UCLA/VA partners with ASU to advance biosensor technology for urinary tract infections
4. Scientists find facial scars increase attractiveness
5. BIO-key(R) Awarded PocketCop(R) Contracts From Major Law Enforcement Agencies
6. L-1 Identity Solutions Selected by the State of New York as the Winning Bidder to Provide Enrollment Services for a Contract Estimated at Up to $250 Million
7. SAIC Awarded $37 Million Contract to Support U.S. Army Program Executive Office - Enterprise Information Systems
8. Evolved Machines Selected as a Prime Contractor for DARPA Program to Engineer an Artificial Olfaction System
9. Alzheimers disease research attracts first partner
10. American College of Medical Genetics receives $13.5M NIH contract
11. What is wild? Odor attraction among different wildtype Drosophila
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/30/2014)... available in German . ... most expensive foods in the world. Because they grow underground, ... the distinctive smell of truffles is not only of interest ... the direction of the Goethe University Frankfurt have discovered that ... bacteria which are trapped inside truffle fruiting bodies. , White ...
(Date:9/30/2014)... involving mice and published in the October 2014 issue ... , suggests that just because you can do it, ... of Italian scientists have found that using marijuana in ... system. This damage may result in autoimmune diseases and ... disease and rheumatoid arthritis in adulthood. , "I hope ...
(Date:9/30/2014)... your doctor to know what goes wrong with your muscles ... to know what "normal" actually is. That,s where a new ... FASEB Journal comes in. In the ... set of molecules that can help scientists "see" which genes ... more, they found never-before-detected gene activity and that men have ...
Breaking Biology News(10 mins):On the trail of the truffle flavor 2Adolescent exposure to thc may cause immune systems to go up in smoke 2Scientists identify which genes are active in muscles of men and women 2
... was only something that was necessary to keep your car ... and animals living in cold climates have natural antifreeze proteins ... matter. Without such antifreeze, living matter would suffer from frost ... proteins is one of the major evolutionary routes taken by ...
... a comprehensive, open-access collection of georeferenced biological information about ... refers to the visible-for-the-eye organisms that live near or ... sponges , ascidians , crustaceans . ... conservation activities on species living near the ocean bottom ...
... that has emerged in the Middle East is well-adapted to ... to a study to be published on February 19 in ... Society for Microbiology. The study indicates that the virus HCoV-EMC ... and evade the innate immune system as easily as a ...
Cached Biology News:Not just cars, but living organisms need antifreeze to survive 2Not just cars, but living organisms need antifreeze to survive 3Data paper describes Antarctic biodiversity data gathered by 90 expeditions since 1956 2Data paper describes Antarctic biodiversity data gathered by 90 expeditions since 1956 3Novel coronavirus well-adapted to humans, susceptible to immunotherapy 2Novel coronavirus well-adapted to humans, susceptible to immunotherapy 3
(Date:9/29/2014)... TORONTO , Sept. 29, 2014 /PRNewswire/ - Portage Biotech ... Exchange: PBT.U), is pleased to announce that its three ... of Portage, who advanced $ 100,000 each in July ... have their Notes and related Coupons converted into 3,500,001 ... and carried 5% coupon. Both the Note and the ...
(Date:9/29/2014)... 2014 Research and Markets  has announced ... Analyzer, and Gas Chromatograph Market by Type , Technology, ... Trends & Forecast to 2013 - 2020" report ... is a process analytical instrument that provides chemical composition ... the way by which companies carry out their production ...
(Date:9/29/2014)... ACEA Biosciences, Inc. (ACEA) has ... iCELLigence Research Grant for his proposal on cancer ... an impedance based real-time label-free cellular analysis instrument. ... Biomedical Engineering at Yale University currently holds a ... Professor Rong Fan's laboratory involve the ...
(Date:9/29/2014)... /PRNewswire/ - SQI Diagnostics Inc. (TSX-V: SQD) (OTCQX: SQIDF), ... technologies and products for advanced microarray diagnostics, is pleased ... to Chairman of the Board of Directors of SQI, ... to serve as a Director on the Board and ... "We are very pleased to have Mr. ...
Breaking Biology Technology:Portage announces conversion of its debt into equity 2Portage announces conversion of its debt into equity 3Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 2Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 3Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 4ACEA Biosciences, Inc. Awards Yale University Researcher the iCELLigence Research Grant 2SQI Diagnostics Announces New Chairman of Board of Directors 2
... Inc. announced today the initiation of a Phase ... inhibitor CX-4945 in patients with multiple myeloma. Cylene ... million financing with participation from existing investors including ... Fund, BioVentures Investors, Lilly Ventures, Mitsui & Co. ...
... GeneLink BioSciences, Inc. (OTC Bulletin Board: GNLK, ... www.helixlife.com ), a private label distribution partnership serving the ... CEO, stated, "HelixLife gives GeneLink reach into new areas ... have not been available to us.  The HelixLife team ...
... and BRUSSELS, Sept. 24 Tarsa Therapeutics, Inc. ... UGNE ) today reported preclinical data showing ... with other drugs in reducing the collagen degradation ... at the 2010 Osteoarthritis Research Society International (OARSI) ...
Cached Biology Technology:Cylene Initiates Phase I Trial in Multiple Myeloma With CX-4945 Inhibitor of CK2 and Closes Accompanying Financing 2Cylene Initiates Phase I Trial in Multiple Myeloma With CX-4945 Inhibitor of CK2 and Closes Accompanying Financing 3GeneLink Announces HelixLife Private Label Distribution Agreement 2Tarsa Therapeutics and Unigene Present Preclinical Data Suggesting Calcitonin May Have Utility in Combination Therapy for the Treatment of Osteoarthritis 2Tarsa Therapeutics and Unigene Present Preclinical Data Suggesting Calcitonin May Have Utility in Combination Therapy for the Treatment of Osteoarthritis 3Tarsa Therapeutics and Unigene Present Preclinical Data Suggesting Calcitonin May Have Utility in Combination Therapy for the Treatment of Osteoarthritis 4Tarsa Therapeutics and Unigene Present Preclinical Data Suggesting Calcitonin May Have Utility in Combination Therapy for the Treatment of Osteoarthritis 5
... deuterium atoms at the 9, 10, 12, and ... an internal standard for the quantification of 13(S)-HODE ... by incubation of linoleic acid with plant and ... the adhesion of tumor cells to the endothelium ...
...
...
Immunogen: Peptide corresponding to amino acid residues from the N-terminal region of human thyroid hormone receptor, alpha1/alpha2-isotype. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Biology Products: