Navigation Links
Successful stem cell differentiation requires DNA compaction, study finds
Date:5/11/2012

o used a rotary suspension culture method developed by McDevitt to produce with high efficiency homogonous 3D clumps of embryonic stem cells called embryoid bodies. Embryoid bodies typically contain cell types from all three germ layers -- the ectoderm, mesoderm and endoderm -- that give rise to the various types of tissues and structures in the body. However, the majority of the H1 triple-knockout embryoid bodies formed in rotary suspension culture lacked differentiated structures and displayed gene expression signatures characteristic of undifferentiated stem cells.

"H1 triple-knockout embryoid bodies displayed a reduced level of activation of many developmental genes and markers in rotary culture, suggesting that differentiation to all three germ layers was affected." noted McDevitt.

The embryoid bodies also lacked the epigentic changes at the pluripotency genes necessary for differentiation, according to Fan.

"When we added one of the deleted H1 subtypes to the embryoid bodies, Oct4 was suppressed normally and embryoid body differentiation continued," explained Fan. "The epigenetic regulation of Oct4 expression by H1 was also evident in mouse embryos."

In another experiment, the researchers provided an environment that would encourage embryonic stem cells to differentiate into neural cells. However, the H1 triple-knockout cells were defective in forming neuronal and glial cells and a neural network, which is essential for nervous system development. Only 10 percent of the H1 triple-knockout embryoid bodies formed neurites and they produced on average eight neurites each. In contrast, half of the normal embryoid bodies produced, on average, 18 neurites.

In future work, the researchers plan to investigate whether controlling H1 histone levels can be used to influence the reprogramming of adult cells to obtain induced pluripotent stem cells, which are capable of differentiating into tissues in a way similar to embry
'/>"/>

Contact: Abby Robinson
abby@innovate.gatech.edu
404-385-3364
Georgia Institute of Technology Research News
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Eye-tracking reveals variability in successful social strategies for children with autism
2. Severe congenital disorder successfully treated in a mouse model for the first time
3. Genomic sequences of 2 iconic falconry birds - Peregrine and Saker Falcons- successfully decoded
4. KAISTs successful transfer of green technology
5. Biodegradable mulches successfully control weeds in container-grown arborvitae
6. Teaching skills key to selection of a successful model farmer
7. National Institutes of Health renews successful infectious disease research study
8. Exonhit and BGI announce successful completion of a promising next-generation sequencing project
9. New substance may allow successful transplantation of marginal livers
10. Successful strategy developed to regenerate blood vessels
11. LSUHSC reports first successful salivary stone removal with robotics
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Successful stem cell differentiation requires DNA compaction, study finds
(Date:10/22/2014)... , Oct. 20, 2014  Leading identity analyst ... time half of the world,s population will have a ... Europe . Asia ... the market accounting for more than 60% of all ... Acuity,s report  -- "The Global National eID ...
(Date:10/18/2014)... patients with undiagnosed, suspected genetic conditions, a certain type ... molecular diagnostic yield than traditional molecular diagnostic methods, according ... study is being released to coincide with the American ... which sequences the protein­coding region of the genome (the ... a cell or organism), has been rapidly applied in ...
(Date:10/16/2014)... the human body. Battles are won, lost or ... this stalemate—known as tumor dormancy—can last up to ... that is poorly understood. , A new ... Torquato, a Professor of Chemistry at Princeton University, ... and the switch to a malignant state. Published ...
Breaking Biology News(10 mins):Half the World's Population Will Have Chip-Based National Electronic Identity (eID) Cards by 2018 2Study examines type of exome sequencing and molecular diagnostic yield 2Study examines type of exome sequencing and molecular diagnostic yield 3Modeling tumor dormancy 2
... temperature fluctuations, such species as corals are only comfortable ... appear to be threatening their survival, according to Drew ... However, she noted in presenting a paper at the ... of Science in San Francisco, Feb. 18, Caribbean gorgonian ...
... to scientific gospel, social behavior is dictated primarily by ... , That may well be true for many adult ... University of Wisconsin-Madison have found evidence that social interactions ... with one another. What's more, the researchers say, the ...
... technology that will allow doctors and scientists to do ... and watch their bones move in 3-D as they ... high-resolution, high-speed imaging system will contribute to better treatments ... scientists understand the evolution of complex movements, from the ...
Cached Biology News:Corals that can fight global warming may one day help fragile reefs 2In young mice, gregariousness seems to reside in the genes 2In young mice, gregariousness seems to reside in the genes 3Bones in motion: Brown scientists to create new 3-D X-ray system 2Bones in motion: Brown scientists to create new 3-D X-ray system 3
(Date:10/22/2014)... October 21, 2014 The Americas ... inorganic refrigerants market in Americas with analysis and ... of the Americas Inorganic Refrigerants Market report, to ... This also provides a glimpse of the segmentation ... supported by various tables and figures. , ...
(Date:10/22/2014)... Involution Studios , a healthcare software design ... Understanding Ebola . The goal of the ... follow informative tool for anyone wanting to know more ... , "As the news has spread in all directions ... not only a healthcare crisis with global impact, but ...
(Date:10/22/2014)... Grace Century, a private equity consultancy ... the addition of Dr. Yousef “Josh” Siddiqui , ... Dr. Siddiqui will provide further healthcare expertise to its ... University College Medical School in the late ... 2001. With further certification as a General Practitioner (GP), ...
(Date:10/22/2014)... ROCKVILLE, Md. , Oct. 22, 2014   ... company developing novel pathogen-specific therapies for serious infections and ... Office has issued a Notice of Allowance for a ... product in its C. difficile program, SYN-004. This ... to SYN-004 in the U.S. and adds to the ...
Breaking Biology Technology:The Americas Inorganic Refrigerants Market is estimated to grow to $71.6 million by 2018 - New Report by MicroMarket Monitor 2The Americas Inorganic Refrigerants Market is estimated to grow to $71.6 million by 2018 - New Report by MicroMarket Monitor 3Involution Studios' Ebola Infographic Provides Key Disease Information and Statistics at a Glance 2Dr. Yousef Siddiqui joins the Grace Century Advisory Team 2Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 2Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 3Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 4
... looking for an ambitious mission statement, consider this pledge from ... simply propose adding new dollars on top of current dollars, ... first those currently in the (K-12 public school) system, and ... the adequacy analysis." , , [Tom Still is a member ...
... a type of atom probe developed by Madison-based Imago ... of individual gold atoms they couldn't have gotten otherwise. ... was used to map the position of atoms in ... of Wisconsin-Madison technology and has sold it since 2003. ...
... Eau Claire, Wis. A digital welding simulator that ... several educational programs was demonstrated on Wednesday at Chippewa ... which was co-developed by the visual technology firm VRSim ... gain hours of welding experience without using up expensive metal ...
Cached Biology Technology:Measuring what works and what doesn't in the prototypical school 2Measuring what works and what doesn't in the prototypical school 3Virtual-reality welding simulator an opportunity for Chippewa Valley college 2
... to Lambda Free Light Chain ... chains of human immunoglobulin. ... expressed epitope of lambda chain. Reacts ... free lambda chains but not with ...
... The Sciclone ALH 4000 workstation is the ... seeking to introduce automated liquid handling capabilities,into ... that need to support routine applications on ... and,integrated for walk-away automation even when ...
NovaScreen offers cell biology, cell culture and molecular biology services for companies who lack a cell culture facility....
Standard and modified oligonucleotides up to 150 nt-long at any scale for broad applications. Price starts from 30p/base (10 nmole scale)....
Biology Products: