Navigation Links
Successful stem cell differentiation requires DNA compaction, study finds
Date:5/11/2012

linker histones and chromatin folding on stem cell differentiation, the researchers used embryonic stem cells that lacked three subtypes of linker histone H1 -- H1c, H1d and H1e -- which is the structural protein that facilitates the folding of chromatin into a higher-order structure. They found that the expression levels of these H1 subtypes increased during embryonic stem cell differentiation, and embryonic stem cells lacking these H1s resisted spontaneous differentiation for a prolonged time, showed impairment during embryoid body differentiation and were unsuccessful in forming a high-quality network of neural cells.

"This study has uncovered a new, regulatory function for histone H1, a protein known mostly for its role as a structural component of chromosomes," said Anthony Carter, who oversees epigenetics grants at NIGMS. "By showing that H1 plays a part in controlling genes that direct embryonic stem cell differentiation, the study expands our understanding of H1's function and offers valuable new insights into the cellular processes that induce stem cells to change into specific cell types."

During spontaneous differentiation, the majority of the H1 triple-knockout embryonic stem cells studied by the researchers retained a tightly packed colony structure typical of undifferentiated cells and expressed high levels of Oct4 for a prolonged time. Oct4 is a pluripotency gene that maintains an embryonic stem cell's ability to self-renew and must be suppressed to induce differentiation.

"H1 depletion impaired the suppression of the Oct4 and Nanog pluripotency genes, suggesting a novel mechanistic link by which H1 and chromatin compaction may mediate pluripotent stem cell differentiation by contributing to the epigenetic silencing of pluripotency genes," explained Fan. "While a significant reduction in H1 levels does not interfere with embryonic stem cell self-renewal, it appears to impair differentiation."

The researchers als
'/>"/>

Contact: Abby Robinson
abby@innovate.gatech.edu
404-385-3364
Georgia Institute of Technology Research News
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Eye-tracking reveals variability in successful social strategies for children with autism
2. Severe congenital disorder successfully treated in a mouse model for the first time
3. Genomic sequences of 2 iconic falconry birds - Peregrine and Saker Falcons- successfully decoded
4. KAISTs successful transfer of green technology
5. Biodegradable mulches successfully control weeds in container-grown arborvitae
6. Teaching skills key to selection of a successful model farmer
7. National Institutes of Health renews successful infectious disease research study
8. Exonhit and BGI announce successful completion of a promising next-generation sequencing project
9. New substance may allow successful transplantation of marginal livers
10. Successful strategy developed to regenerate blood vessels
11. LSUHSC reports first successful salivary stone removal with robotics
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Successful stem cell differentiation requires DNA compaction, study finds
(Date:9/21/2014)... (black-eyed peas) is a fascinating example of how science ... the story of the scientist behind the research. ... the 21st Century to serve as an accessible summary ... recently published by the Crop Science Society of America, ... , Singh has devoted his life,s work to solving ...
(Date:9/21/2014)... MA -- In recent years, new strains of bacteria ... Each year, these superbugs, including drug-resistant forms of tuberculosis ... and kill at least 23,000. Despite the urgent need ... classes of antibiotics in the past decade. , MIT ... these superbugs. Using a gene-editing system that can disable ...
(Date:9/21/2014)... a protein therapy that disrupts the process that causes ... travel through the blood stream and start aggressive new ... as metastasis, can cause cancer to spread with deadly ... to cancer fall prey to metastatic forms of the ... who describes a new therapeutic approach in Nature ...
Breaking Biology News(10 mins):Battling superbugs 2Battling superbugs 3Battling superbugs 4Stanford researchers create 'evolved' protein that may stop cancer from spreading 2Stanford researchers create 'evolved' protein that may stop cancer from spreading 3Stanford researchers create 'evolved' protein that may stop cancer from spreading 4
... long puzzled scientists, and several scenarios have been proposed ... group appeared virtually simultaneously on Asia, Europe and North ... new research using the latest evidence suggests a completely ... that sudden, rapid global warming drove the dispersal. ...
... Researchers at the Burnham Institute for Medical Research have ... free radicals and accumulation of misfolded proteins that lead ... such as Alzheimer's and Parkinson's Disease. , ... protein that is necessary for proper protein folding in ...
... The study of 18 HIV-positive subjects shows that HIV in ... HIV that lives in the blood and peripheral tissues. , ... with the presence of a particular mutation in the HIV ... 2006 issue of Brain. It was led by Satish K. ...
Cached Biology News:Ancient global warming drove early primates' dispersal 2Key stress protein linked to toxicities responsible for Parkinson's, Alzheimer's 2Viral genetic differences are possible key to HIV dementia 2Viral genetic differences are possible key to HIV dementia 3
(Date:9/23/2014)... The Minnesota High Tech Association ... cellular modems , platforms and application-specific remote monitoring ... for the 2014 Tekne Awards. The awards ... Convention Center on Thursday, November 13th. The Tekne ... shown superior technology innovation and leadership. , ...
(Date:9/22/2014)... Pearl's Premium Ultra Low Maintenance Lawn Seed ... seed this week with the release of their Pearl's ... the fact that it is time to plant now ... , As of the date 6/4/13, Pearl's Premium had ... experts and the media including: The National Gardeners ...
(Date:9/22/2014)... Thanks to new LEGO-like components developed by researchers ... is now possible to build a 3-D microfluidic ... small modules by hand. , Microfluidic systems ... and biotechnology to precisely manipulate small volumes of ... or DNA analysis, pathogen detection, clinical diagnostic testing, ...
(Date:9/22/2014)... a new star-shaped molecule made up of interlocking rings, which ... , Known as a ,Star of David, molecule, scientists ... of a century and the team,s findings are published in ... two molecular triangles, entwined about each other three times into ... triangle is 114 atoms in length around the perimeter. The ...
Breaking Biology Technology:NimbeLink Named Finalist for 15th Annual Tekne Awards 2NimbeLink Named Finalist for 15th Annual Tekne Awards 3Pearl's Premium Ultra Low Maintenance Lawn Seed Announces A Major Breakthrough In Drought Tolerant Grass Seed 2Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 2Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 3Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 4Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 5
... Finger Nuclease Platform enables Precise, Permanent, and ... Heritable Modification of the Genome, ... CompoZr(TM) platform of zinc finger nuclease (ZFN),reagents. The CompoZr ZFN ... to target and precisely manipulate the genome,of living cells, resulting ...
... plant-based extract, is better than metformin, the leading Type 2 diabetes ... ... Winter Haven, FL (PRWEB) September 16, 2008 -- A ... 2 diabetics better than a leading prescription drug, according to the ...
... Development of Gene Expression Signatures Derived by COXEN ... Molecular Test of Responsiveness to Current Treatments, ... Transgenomic (OTC Bulletin Board: TBIO) today announced a ... test which will guide,clinicians in their choice of ...
Cached Biology Technology:Sigma-Aldrich Launches Breakthrough Genome-Editing Tools 2Sigma-Aldrich Launches Breakthrough Genome-Editing Tools 3Human Trial Results Show Plant Extract Tops Drug at Regulating Blood Sugar Levels; Huge Market Seen for Newly Developed Food Additive Emulin™ 2Transgenomic Announces Partnership With Key Genomics for Ovarian Cancer Assay to Guide Therapeutic Options 2Transgenomic Announces Partnership With Key Genomics for Ovarian Cancer Assay to Guide Therapeutic Options 3Transgenomic Announces Partnership With Key Genomics for Ovarian Cancer Assay to Guide Therapeutic Options 4
... is proud to work in collaboration with ... 25mm syringe filters in a variety of ... licensed for use on the Caliper TPWII, ... and Pricing for our Automation Certified Syringe ...
... 3 Dodeca cell with multi-casting chamber is used ... 12 mini format handcast (8.3 x 7.3 cm) ... cell requires 3.4-4.4 L of buffer and includes ... attaches to an external refrigerated circulator (not included), ...
...
... This product has been developed to ... peroxidase labeled proteins. Stability is increased ... to 2-8 centigrade. This product is ... no mercury or azide. This formulation ...
Biology Products: