Navigation Links
Study validates means to measure possible leukemia marker
Date:4/30/2009

COLUMBUS, Ohio A study led by cancer researchers at The Ohio State University has validated a method for reliably measuring variations in certain proteins that may make good biomarkers in chronic leukemia patients.

The study shows that liquid chromatography-mass spectrometry (LC-MS) can measure variations in histones, which are spool-like proteins that help support and store DNA. The technology accurately detected differences in the composition of histones in chronic lymphocytic leukemia (CLL) cells compared with their healthy counterparts, immune cells called B lymphocytes.

These variations are a promising molecular biomarker that might improve the diagnosis and gauge response to therapy in CLL patients.

The findings are published online in a recent issue of the journal Proteomics.

"Now that we have validated the technique, we can apply it to study histones as biomarkers that clinicians can use to make decisions about diagnosis and treatment," says principal investigator Michael A. Freitas, a researcher in the Ohio State University Comprehensive Cancer Center's Experimental Therapeutics Program.

Freitas and his colleagues used LC-MS to analyze variations in histones in 40 CLL patients and four healthy patients. It showed significant changes in the relative abundance of histone H2A variants in CLL cells compared with healthy B cells and suggested a correlation between these changes and the severity of CLL.

To verify the accuracy of their measurements, the investigators tested the technology against common sources of error that can arise in clinical testing laboratories. These included analyzing samples prepared in different ways and by different people, and using different instruments. They also re-analyzed samples that were nearly two years old.

"All of these can be huge problems in biomarker analysis," Freitas says. "The danger is that patients can end up being separated into groups based on changes in how the analysis is done rather than on actual changes in the biomarker.

"We still need to be concerned about experimental bias," he says, "but this study demonstrates that we can detect real biological differences in histones in CLL cells, as opposed to methodological differences."


'/>"/>

Contact: Darrell E. Ward
Darrell.Ward@osumc.edu
614-293-3737
Ohio State University Medical Center
Source:Eurekalert

Related biology news :

1. Childhood obesity indicates greater risk of school absenteeism, Penn study reveals
2. A study by the MUHC and McGill University opens a new door to understanding cancer
3. Study begins to reveal clues to the cause and progression of sepsis
4. Clones on task serve greater good, evolutionary study shows
5. New study warns limited carbon market puts 20 percent of tropical forest at risk
6. New study examines how rearing environment can alter navigation
7. Study links cat disease to flame retardants in furniture and to pet food
8. New continent and species discovered in Atlantic study
9. Study shows link between alcohol consumption and hiv disease progression
10. Feeling hot, hot, hot: New study suggests ways to control fever-induced seizures
11. Study finds environmental tests help predict hospital-acquired Legionnaires disease risk
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/16/2017)... May 16, 2017  Veratad Technologies, LLC ( www.veratad.com ... age and identity verification solutions, announced today they will ... 2017, May 15 thru May 17, 2017, in ... International Trade Center. Identity impacts the ... in today,s quickly evolving digital world, defining identity is ...
(Date:4/19/2017)... York , April 19, 2017 ... as its vendor landscape is marked by the presence ... market is however held by five major players - ... Together these companies accounted for nearly 61% of the ... the leading companies in the global military biometrics market ...
(Date:4/13/2017)... According to a new market research report "Consumer IAM Market ... Authorization), Service, Authentication Type, Deployment Mode, Vertical, and Region - Global Forecast ... from USD 14.30 Billion in 2017 to USD 31.75 Billion by 2022, ... ... MarketsandMarkets Logo ...
Breaking Biology News(10 mins):
(Date:8/16/2017)... (PRWEB) , ... August 16, 2017 , ... ... beneficial microbe delivery system, announced it has secured $2M in funding from an ... City Angels, Carmen Innovations, and SVG Thrive Fund. With this investment, 3Bar is ...
(Date:8/15/2017)... ... ... Kapstone Medical is proud to announce that it has reached ... inventors develop and safeguard their latest innovations. The company has grown from a ... clients in the United States and around the world. , Company Founder and ...
(Date:8/15/2017)... ... , ... The Conference Forum and The Trout Group ... of upcoming panels and events. The partnership culminates with the 4th Annual IO360° ... York City. , “With our experience in producing the Immuno-Oncology 360° NYC event and ...
(Date:8/14/2017)... , ... August 14, 2017 , ... The Conference ... event, which will take place on September 6, 2017 at the Marriott Copley ... , Head of Experimental Medicine, Informatics, and Regulatory Strategy, Pfizer Innovative Research Lab, Pfizer, ...
Breaking Biology Technology: