Navigation Links
Study shows how the zebrafish gets his stripe
Date:9/25/2007

Scientists have discovered how the zebrafish (Danio rerio) develops one of its four stripes.

Their findings add to the growing list of tasks carried out by an important molecule that is involved in the arrangement of everything from nerve cells to reproductive cells in the developing embryo.

The research focused on a particular zebrafish mutant known as choker, which is distinctive because one of the four stripes running down its side is missing, and it has a dark collar around its neck instead.

Dark spots and stripes in fish, amphibians and reptiles are usually caused by a type of cell, known as a melanophore, which contains high quantities of the pigment melanin.

Using time-lapse photography, the team put together movies showing how the melanophores migrate in developing embryos of both the wild-type (naturally occurring) zebrafish and the choker mutant.

At first, cells migrate through the neck region in both wild-type and choker mutant fish to generate two stripes. Then, whilst cell migration ceases in the neck region of the wild-type embryo, melanophores in the choker mutant exit from the two stripes and busily cluster around the collar region of the developing fish.

It is as though someone has put up keep off the grass signs in the wild-type zebrafish to keep the melanophores in separate paths (stripes), said Dr Robert Kelsh from the University of Bath who led the study which was published in the journal Development.

The melanophores stay on the footpath of the developing stripe in a very orderly fashion, but in choker its as though these signs have been knocked down, and the pigment cells run all over the grass between the footpaths.

The breakthrough in understanding how this happens came with the discovery that a molecule, known as Sdf, appears to have some role in encouraging melanophore cell migration.

Sdf is a known cell migration guidance molecule, with roles including regulating the migration of neurones in the fishs central nervous system and germ (reproductive) cells.

When researchers looked where the molecule was found, they could see that it appeared to line up in the same pattern as a third stripe in the wild-type zebrafish, and around the collar in choker mutants.

The correlation of Sdf expression and melanophore pattern in wild-type and choker mutants embryos was so striking, it immediately suggested that Sdf plays a key role in dictating the pattern of at least this one stripe in zebrafish, said Dr Kelsh.

To test the theory, researchers positioned a bead soaked in human Sdf onto the skin of zebrafish embryo. Even in the choker mutant, this bead attracted the melanophores to where it was implanted.

The researchers also used a technique known as protein knockdown to reduce the amount of Sdf produced in the embryo, and were able to partially remove the third stripe in wild types and to reduce colouring in the neck of the choker mutant.

Sdf is really important, not just for pigmentation but also the development of neurons and germ cells, said Dr Kelsh.

The other three stripes must have some other mechanism controlling their development. We are still looking into how these other three stripes might form.

Pigment pattern formation is a classic problem in developmental biology and whilst there have been lots of mathematical theories on how the fish make different patterns, the underlying genetics have been unclear.

Similarities between animals mean that we can take what we learn about the development of these simple models, and begin applying them to more complex systems, such as humans.


'/>"/>

Contact: Andrew McLaughlin
a.mclaughlin@bath.ac.uk
44-012-253-86883
University of Bath
Source:Eurekalert

Related biology news :

1. Bioartificial kidney under study at MCG
2. Novel Asthma Study Shows Multiple Genetic Input Required; Single-gene Solution Shot Down
3. W.M. Keck Foundation funds study of friendly microbes
4. Yellowstone microbes fueled by hydrogen, according to U. of Colorado study
5. Emory Study Tests Bone Marrow Stem Cells to Improve Circulation in Legs
6. UCLA Study Shows One-Third of Drug Ads in Medical Journals Do Not Contain References Supporting Medical Claims
7. Study Demonstrates Gene Expression Microarrays are Comparable and Reproducible
8. Study Links Ebola Outbreaks To Animal Carcasses
9. Genome-wide mouse study yields link to human leukemia
10. Breakthrough Microarray-based Technology for the Study of Cancer
11. NYU Study Reveals How Brains Immune System Fights Viral Encephalitis
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/11/2017)... 11, 2017 NXT-ID, Inc. (NASDAQ:   ... announces the appointment of independent Directors Mr. Robin D. ... Board of Directors, furthering the company,s corporate governance and expertise. ... Gino Pereira , ... forward to their guidance and benefiting from their considerable expertise ...
(Date:4/5/2017)... 2017 Today HYPR Corp. , leading ... component of the HYPR platform is officially FIDO® ... security architecture that empowers biometric authentication across Fortune 500 ... secured over 15 million users across the financial services ... home product suites and physical access represent a growing ...
(Date:4/3/2017)... 3, 2017  Data captured by IsoCode, ... detected a statistically significant association between the ... treatment and objective response of cancer patients ... predict whether cancer patients will respond to ... well as to improve both pre-infusion potency testing ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... , ... AMRI, a global contract research, development and manufacturing ... and quality of life, will now be offering its impurity solutions as a ... requirements for all new drug products, including the finalization of ICH M7 earlier ...
(Date:10/11/2017)... ... October 11, 2017 , ... At its national board meeting ... I. Sheikh, the co-founder, CEO and chief research scientist of Minnesota-based Advanced Space ... membership in ARCS Alumni Hall of Fame . ASTER Labs is a ...
(Date:10/11/2017)... ... 11, 2017 , ... Personal eye wash is a basic first aid supply for any work ... which eye do you rinse first if a dangerous substance enters both eyes? It’s one ... with its unique dual eye piece. , “Whether its dirt and debris, or an ...
(Date:10/11/2017)... ... October 11, 2017 , ... ... will be hosting a Webinar titled, “Pathology is going digital. Is your lab ... digital pathology adoption best practices and how Proscia improves lab economics and realizes ...
Breaking Biology Technology: