Navigation Links
Study offers new clues about hereditary spastic paraplegia
Date:7/8/2011

HOUSTON -- (July 8, 2011) -- New research from Rice University and Italy's Eugenio Medea Scientific Institute is yielding clues about hereditary spastic paraplegia (HSP), a group of inherited neurological disorders that affect about 20,000 people in the United States. A study in the July 5 issue of the Proceedings of the National Academy of Sciences offers the first detailed account of the biochemical workings of atlastin, a protein produced by one of the genes linked to HSP.

The primary symptoms of HSP are progressive spasticity and weakness of the leg and hip muscles. This results from the slow degeneration of the nerves that carry signals from the spinal column to the legs, feet and toes. While scientists know that HSP can result from more than two dozen different genetic mutations, they don't know how the mutations cause nerve degeneration.

"We discovered a couple of years ago that atlastin plays a key role in building and maintaining an important internal compartment of healthy cells called the endoplasmic reticulum," said Rice biochemist James McNew, lead co-author of the new study. "HSP is known to primarily affect long nerve cells that can stretch from the lower back all the way to the base of the leg. It appears that atlastin plays a particularly crucial role in maintaining the health of these cells, and we want to know why."

McNew, associate professor of biochemistry and cell biology at Rice, and Andrea Daga, a scientist at the Medea Institute, determined in 2009 that atlastin was one of the rare breed of proteins that can cause membrane fusion.

"Membrane fusion is a fundamental process involved in many cell functions, but only a few proteins can initiate it," McNew said. "Until 2009, we thought all membrane fusion proteins operated in the same basic way. Atlastin was completely different because it's an enzyme that utilizes chemical energy to drive fusion. We really had to start from scratch to determine how it worked."

Membranes are both the brick walls and the zip-lock bags of microbiology. Every living cell is encased in a water-tight membrane of fatty acids, and all of the cell's interactions with the outside world are mediated by what can and cannot pass through that membrane. When a virus invades a healthy cell, it uses membrane fusion. When a sperm fertilizes an egg, it uses membrane fusion. Membranes are also used inside the cell, where they serve as biochemical reaction chambers, as cargo containers and as pipelines.

To find out how atlastin initiates membrane fusion, McNew, Daga and colleagues conducted experiments involving the fruit-fly version of atlastin, which is remarkably similar to the version found in humans. By selectively disabling portions of the protein and examining how those modifications affected atlastin's ability to promote membrane fusion in the test tube and cell cultures, the team gradually built a picture of how atlastin works. They found that pairs of atlastin proteins, each of which is anchored in a separate membrane, can bind to one another when both partners are functional enzymes. That action draws the separated membranes together and allows fusion to occur.

Additionally, they found that a small piece near the end of atlastin was required for fusion activity.

"Atlastin has a fairly short tail, which protrudes from the site where the protein is anchored into the membrane," Daga said. "When we deleted this tail, we found that the protein could not stimulate membrane fusion, even when the two copies bound together normally."

McNew said prior gene sequencing studies have show that some HSP patients have genetic mutations that result in defects in this critical tail region of the atlastin protein.

"This study, as well as work by others, suggests the area of the cell where atlastin promotes membrane fusion, the endoplasmic reticulum, is a good target for future studies into the causes of HSP," said McNew, a faculty investigator at Rice's BioScience Resesarch Collaborative.


'/>"/>

Contact: Jade Boyd
jadeboyd@rice.edu
713-348-6778
Rice University
Source:Eurekalert  

Related biology news :

1. Study suggests new strategy to prevent infertility, birth defects
2. UTMB-led researchers awarded $7.8 million for Gulf spill study
3. LSUHSC conducts landmark study of mid, long-term health effects of oil exposure
4. Nordic study shows marginally higher but overall low risk of stillbirth in ART children
5. First whole-genome lung cancer study by TGen and Virginia G. Piper Cancer Center set for conference
6. Twin study shows lifestyle, diet can significantly influence course of macular degeneration
7. Study: Preventive use of one form of natural vitamin E may reduce stroke damage
8. Behavioral treatment for migraines a cost-effective alternative to meds, study finds
9. ARS and cooperators study cotton gin dust emissions
10. Study finds pregnancy and birth environment may affect development of autism in twins
11. Study shows answers for treating obesity-related diseases may reside in fat tissue
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Study offers new clues about hereditary spastic paraplegia
(Date:2/2/2016)... --Technology Enhancements Accelerate Growth of X-ray Imaging This ... computed radiography markets in Thailand , ... (TIM). It provides an in-depth analysis of ... regional market drivers and restraints. The study offers revenue ... attractiveness, both for digital and computed radiography. Market participants ...
(Date:2/1/2016)... -- Wocket® smart wallet ( www.wocketwallet.com ) announces the launch of a new ... Las Vegas , where Joey appeared at the ... Las Vegas , where Joey appeared at the Wocket booth to ... was filmed at the Consumer Electronics Show (CES2016) in Las ... and greet fans. --> --> ...
(Date:1/22/2016)... DUBLIN , January 22, 2016 ... has announced the addition of the  ... to their offering. --> ... of the  "Global Behavioral Biometric Market ... --> Research and Markets ( http://www.researchandmarkets.com/research/4lmf2s/global_behavioral ...
Breaking Biology News(10 mins):
(Date:2/10/2016)... Feb. 10, 2016  IsoRay, Inc. (NYSE MKT: ISR), ... and medical radioisotope applications for the treatment of prostate, ... announced its financial results for the second quarter and ... 2015. --> --> ... of fiscal 2016, which ended December 31, 2015, a ...
(Date:2/10/2016)... ... 10, 2016 , ... LATHAM, NEW YORK... Marktech Optoelectronics will feature ... in San Francisco’s Moscone Center from February 16-18, 2016, and at the healthcare-focused ... latest InGaAs PIN diode standard packages feature a TO-46 metal can with active areas ...
(Date:2/10/2016)... ... 2016 , ... Cenna Bioscience Inc., an emerging biopharmaceutical company focused on the ... it has been selected to present at the Cavendish Global Health Impact Forum taking ... The purpose of the Forum is to help family offices and foundations develop and ...
(Date:2/9/2016)... ... February 08, 2016 , ... ... announced today the launch of its revamped and improved website. In an on-going ... solutions, the redesigned website will better communicate how the company designs and delivers ...
Breaking Biology Technology: