The researchers grafted developing hippocampal tissue to middle-aged recipient rats knowing that the grafts may be compromised by increased systemic cytokines and that the HFHC diet would be further detrimental.
After six weeks, the researchers found stunted growth in fetal hippocampal tissues among the animals fed the HFHC diet as opposed to normal development in controls receiving a normal diet. However, when treated topically with IL-1Ra, the HFHC fed animals grew grafts three times the size of HFHC-fed, saline-treated controls.
"All factors taken together, the HFHC diet was shown to exert detrimental effects on hippocampal growth and development," explained Dr. Freeman. "Factors contributing to this effect include, but are not limited to, vascularization and inflammation."
The research team concluded that not only was graft growth altered by the HFHC diet, but that neuronal development and organization was affected as well. However, that treatment with IL-1Ra blocked the detrimental effects of the HFHC diet on hippocampal tissues may indicate a potential use for IL-1Ra in neurological disorders involving neuroinflammation.
"Eight weeks on a Westernized high fat high cholesterol diet causes developmental neuroinflammation in middle-aged rats that can be reversed by an interleukin-1 receptor antagonist" said Dr. John Sladek, professor of neurology and pediatrics at the University of Colorado School of Medicine. "It will be interesting to see if the interleukin-1 receptor antagonist is still effective with a long term Westernized diet and whether this can be translated to humans."
|Contact: David Eve|
Cell Transplantation Center of Excellence for Aging and Brain Repair