Navigation Links
Study analyzes role of PARP enzyme in eukaryotes
Date:3/8/2011

An Ohio State University molecular biologist leveraged a supercomputer to help better define the family tree of a group of enzymes that have been implicated in a wide range of human diseases and are important targets for anti-cancer therapies.

Along with several OSU colleagues, Rebecca S. Lamb, Ph.D., an assistant professor of Molecular Genetics, recently analyzed the evolutionary history of the poly(ADP-ribose)polymerase (PARP) superfamily.

These proteins are found in eukaryotes, a wide range of organisms animals, plants, molds, fungi, algae and protozoa whose cells contain complex structures enclosed within membranes. While PARP proteins can be found with any of these "supergroups," they have been most extensively studied in mammals.

"In these organisms, PARPs have key functions in DNA repair, genome integrity and epigenetic regulation," said Lamb. "More recently it has been found that proteins within the PARP superfamily have a broader range of functions that initially predicted."

The researchers used computers to identify 236 PARP proteins from 77 species across five of the six supergroups. Lamb then accessed the Glen Cluster at the Ohio Supercomputer Center (OSC) to perform extensive phylogenetic analyses of the identified PARP regions.

"This is computationally intensive work that would have been impossible without the computer resources provided at OSC," Lamb said. "In particular, the ability to try a variety of tools that require a great deal of CPU and memory capabilities was essential for success."

Amongst other tools, she employed the PhyML3.0 software package, which fit a statistical model to the aligned sequence data and provided estimates for the model's parameters.

"Dr. Lamb's project is an excellent example of a scientist running a very domain-specific software package on our state-of-the-art systems," said Ashok Krishnamurthy, Ph.D., interim co-executive director of OSC. "While the center maintains a large collection of licensed and open-source software, there are occasions where very specialized or customized applications are required. Our staff members are very good at working with researchers to modify these codes to get them installed, running and delivering results."

Access to powerful OSC systems allowed the researchers to experiment with a wide variety of options and parameters, in order to achieve the best results, Lamb noted.

"PARPs are found in all eukaryotic supergroups for which sequence is available, but some individual lineages within supergroups have independently lost these genes," said Lamb. "The PARP superfamily can be subdivided into six branches or 'clades.' Two of these clades were likely found in the last common eukaryotic ancestor. In addition, we have identified PARPs in organisms in which they have not previously been described."

Three main conclusions were drawn from the study. First, the broad distribution and pattern of representation of PARP genes indicated to the researchers that the ancestor of all existing eukaryotes encoded proteins of this type. Second, the ancestral PARP proteins had different functions and activities. One of these proteins likely functioned in DNA damage response. Third, the diversity of the PARP superfamily is larger than previously documented, suggesting as more eukaryotic genomes become available, this gene family will grow in both number and type.


'/>"/>

Contact: Mr. Jamie Abel
jabel@osc.edu
614-292-6495
Ohio Supercomputer Center
Source:Eurekalert  

Related biology news :

1. Fossil bird study describes ripple effect of extinction in animal kingdom
2. University of Missouri researcher study provides insight into how corn makes hormones
3. Rainwater harvest study finds roofing material affects water quality
4. A study reveals the keys to the locomotion of snails
5. Pathology study tracks uterine changes with mifepristone
6. David and Goliath viruses shed light on the origin of jumping genes: UBC study
7. Scientists study control of invasive tree in western US
8. UF Pine lsland pollen study leads to revision of states ancient geography
9. Nature study: Jefferson researchers unravel proteins elusive role in embryo and disease development
10. Combined molecular study techniques reveal more about DNA proteins
11. New study suggests ALS could be caused by a retrovirus
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Study analyzes role of PARP enzyme in eukaryotes
(Date:2/2/2016)... , Feb. 2, 2016  Based on its ... & Sullivan recognizes US-based Intelligent Retinal Imaging Systems ... Sullivan Award for New Product Innovation. IRIS, a ... North America , is poised to ... growing diabetic retinopathy market. The IRIS technology presents ...
(Date:1/28/2016)... JOSE, Calif., Jan. 28, 2016 Synaptics (NASDAQ: SYNA ... results for its second quarter ended December 31, 2015. ... second quarter of fiscal 2016 increased 2 percent compared to the ... second quarter of fiscal 2016 was $35.0 million, or $0.93 per ... Non-GAAP net income for the first quarter of fiscal 2016 grew ...
(Date:1/22/2016)... , January 22, 2016 /PRNewswire/ ... announced the addition of the  "Global ... their offering. --> http://www.researchandmarkets.com/research/4lmf2s/global_behavioral ... the  "Global Behavioral Biometric Market 2016-2020" ... Research and Markets ( http://www.researchandmarkets.com/research/4lmf2s/global_behavioral ) ...
Breaking Biology News(10 mins):
(Date:2/10/2016)... ... February 10, 2016 , ... SonaCare ... training and support program, Sonalinkā„¢ remote monitoring. The inaugural launch of this new ... February 5th, connecting Dr. Samuel Peretsman to a HIFU technical expert at SonaCare ...
(Date:2/10/2016)... BC (PRWEB) , ... February 09, 2016 , ... ... design services and current winner of the Highest Overall Customer Rating Award ... in all of its business units across the USA, Canada, Mexico and China. ...
(Date:2/9/2016)... ... February 09, 2016 , ... ... innovations on its free and validated Electronic Data Capture (EDC) system ClinCaptureand its ... in Clinical Trials West Coast 2016 Conference in San Mateo, California on February ...
(Date:2/9/2016)... , Feb. 9, 2016  DNAtrix, ... immunotherapies for cancer, announced that its lead ... European Commission as an orphan medicinal product ... deadliest form of glioma, strikes approximately 25,000 ... EU. http://photos.prnewswire.com/prnh/20160208/330986LOGO ...
Breaking Biology Technology: