Navigation Links
Study: Preventive use of one form of natural vitamin E may reduce stroke damage
Date:7/5/2011

COLUMBUS, Ohio Ten weeks of preventive supplementation with a natural form of vitamin E called tocotrienol in dogs that later had strokes reduced overall brain tissue damage, prevented loss of neural connections and helped sustain blood flow in the animals' brains, a new study shows.

Researchers say the findings suggest that preventive, or prophylactic, use of this natural form of vitamin E could be particularly helpful to people considered at highest risk for a major stroke: those who have previously suffered a ministroke, or a temporary stoppage of blood flow in the brain.

Of the almost 800,000 strokes in the United States each year, an estimated 25 percent are repeat events, according to the American Heart Association.

Vitamin E occurs naturally in eight different forms, and this work led by Ohio State University scientists is focused on the tocotrienol form, also known as TCT. The commonly known form of vitamin E belongs to a variety called tocopherols. TCT is not abundant in the American diet but is available as a nutritional supplement. It is a common component of a typical Southeast Asian diet.

In the study, 24 hours after a stroke, lesions indicating brain tissue damage were about 80 percent smaller in dogs that received supplementation than were the lesions in dogs that received no intervention. Imaging tests showed that the treated animals' brains had better blood flow at the stroke site as compared to untreated dogs' brains, a difference attributed to tiny collateral blood vessels' ability to improve circulation in the brain when blood flow stopped in more substantial vessels.

"For the first time, in this pre-clinical large-animal model, we were able to see something that we were never able to see in the mouse or the rat: that if you had a stroke and you had prophylactically taken tocotrienol, the area of the brain affected by the stroke received blood flow from the collaterals," said Chandan Sen, professor and vice chair for research in Ohio State's Department of Surgery and senior author of the study. "These collaterals, which are an emergency response system, wake up when the blood circulation in the brain is challenged."

Sen and colleagues have spent the past 10 years documenting in cell cultures and rodents how this form of vitamin E protects brain cells from dying after the insult of a stroke. They say that the results of this large-animal study offer the last piece of evidence needed to validate testing the nutritional supplement's protection against stroke in humans. A phase II trial of its effectiveness in humans is in the planning stages.

The research is published online and is scheduled for future print publication in the Journal of Cerebral Blood Flow & Metabolism.

In the study, 20 dogs were randomly assigned to one of two groups: those receiving a placebo pill, and those receiving 200 milligrams of mixed tocotrienols. Though alpha-tocotrienol is the form of the vitamin known for its protection of brain cells, the supplement for this study contained a mix of tocotrienols to make it more accessible and affordable.

The dogs ate regular food and received two supplement pills per day for 10 weeks. At this point, scientists induced stroke by blocking the middle cerebral artery in the animals' brains for one hour while the animals were under anesthesia.

The researchers used a variety of imaging techniques to examine the effects of the stroke on the two groups. Magnetic resonance imaging (MRI) showed the differences in the volume of tissue damaged by the stroke. One hour after the stroke, the lesions in the treated dogs' brains were about 60 percent smaller than the size of the lesions in the untreated brains. Twenty-four hours after the stroke, the lesions were 80 percent smaller in treated animals compared to untreated animals.

In collaboration with the Ohio Supercomputer Center, the scientists mapped the brain's communication network, represented by white matter fiber pathways. The sophisticated video images of the stroke-affected brain showed major gaps in this fiber network in the brains of animals that received no supplementation. The fiber connections were protected in the brains of dogs that received TCT.

Images of the blood vessels in the animals' brains showed different responses in the brain blood circulation based on whether or not they had received preventive treatment. The researchers used a scoring system to determine how much of the collateral circulatory system was activated in response to the blocked blood flow associated with the stroke. The score in treated dogs was almost twice as high as that in untreated animals.

"This function in the brain is similar in humans and large mammals, which underscores the significance of these findings," said Cameron Rink, first author of the study and an assistant professor of vascular surgery at Ohio State.

Additional examination of the affected brain tissue showed that the TCT supplementation appeared to support arteriogenesis, a process by which collateral arteries remodel themselves into larger vessels so they can bypass the site of blockage. Genes associated with this process were more active in the affected brain tissue from treated animals than were those from untreated dogs.

Preventive use of TCT, a natural vitamin, is safe and should be embraced as a preventive therapy along with aspirin, a commonly prescribed medicine to prevent a stroke, said Sen, who is also a deputy director of Ohio State's Davis Heart and Lung Research Institute.

"Though most of us have no idea if we are at risk for stroke, a sizable population has had a ministroke and is therefore at high risk for a large stroke. If I had a ministroke, why would I not take something that would minimize my damage during a stroke?" Sen said. "And this is not a drug; it is a nutritional countermeasure. So there are no worries about side effects. Therefore I see it as having prophylactic value."


'/>"/>

Contact: Chandan Sen
chandan.sen@osumc.edu
614-247-7786
Ohio State University
Source:Eurekalert

Related biology news :

1. Study: Wild Cuban crocodiles hybridize with American crocs
2. Smithsonian study: Stranding records are faithful reflection of live whale and dolphin populations
3. Study: Biodegradable products may be bad for the environment
4. MIT Study: conventional fossil fuels sometimes greener than biofuels
5. Study: Pace of brain development still strong in late teens
6. Study: Rare deep-sea starfish stuck in juvenile body plan
7. U of I study: Before you start bone-building meds, try dietary calcium and supplements
8. Study: Reasonable quantities of red pepper may help curb appetite
9. VIMS study: Propeller turbulence may affect marine food webs
10. Study: Algae could replace 17 percent of US oil imports
11. Study: Emissions trading doesnt cause pollution hot spots
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/22/2016)... , June 22, 2016  The American College of Medical ... Show Executive Magazine as one of the fastest-growing trade ... 25-27 at the Bellagio in Las Vegas ... highest percentage of growth in each of the following categories: ... companies and number of attendees. The 2015 ACMG Annual Meeting ...
(Date:6/21/2016)... 21, 2016 NuData Security announced today that ... of principal product architect and that Jon ... customer development. Both will report directly to ... moves reflect NuData,s strategic growth in its product ... customer demand and customer focus values. ...
(Date:6/15/2016)... York , June 15, 2016 ... new market report titled "Gesture Recognition Market by Application ... Forecast, 2016 - 2024". According to the report, the  ... 11.60 billion in 2015 and is estimated to ... USD 48.56 billion by 2024.  Increasing ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... -- A person commits a crime, and the detective uses ... criminal down. An outbreak of foodborne illness makes ... uses DNA evidence to track down the bacteria that caused ... not. The FDA has increasingly used a complex, cutting-edge technology ... Put as simply as possible, whole genome sequencing is a ...
(Date:6/23/2016)... SAN FRANCISCO , June 23, 2016   ... it has secured $1 million in debt financing from ... to ramp up automation and to advance its drug ... for its new facility. "SVB has been ... goes beyond the services a traditional bank would provide," ...
(Date:6/23/2016)... NEWPORT BEACH, Calif. , June 23, 2016 /PRNewswire/ ... offering new biological discoveries to the medical community, has ... and co-founder Matthew Nunez . "We ... provide us with the capital we need to meet ... funding will essentially provide us the runway to complete ...
(Date:6/23/2016)... ... 2016 , ... Velocity Products, a division of Morris Group, ... exclusively for Okuma CNC machining centers at The International Manufacturing Technology Show, IMTS, ... companies with expertise in toolholding, cutting tools, machining dynamics and distribution, Velocity SMART ...
Breaking Biology Technology: