Navigation Links
Studies on imaging and tracking transplanted cells
Date:12/10/2008

Tampa, Fla. (Dec. 10, 2008) Successfully monitoring the distribution and fate of transplanted stem cells through imaging and subsequent tracking would aid clinicians in their ability to evaluate the efficacy of transplanted cells. Three studies published in the current issue of Cell Transplantation (Vol. 17, No.8) assess various imaging techniques and evaluate the degree to which the techniques afford clinicians useable imaging and tracking data.

Bioluminescent imaging of human fetal hepatocytes

In a study conducted by Dr. Mark A. Zern and colleagues at the Transplant Research Institute, University of California Davis Medical Center, the use of bioluminescent imaging to track transplanted immortalized human fetal hepatocytes injected into mice was evaluated. According to the researchers, it was essential for them to evaluate via noninvasive monitoring the effectiveness of transplanted cells to engraft and repopulate the recipient liver. Using double or triple fusion lentiviral vectors in a mouse model transplanted with immortalized fetal hepatocytes, the researchers for the first time imaged in vivo human hepatic progenitor cells transplanted into a rodent model.

"Our results, confirmed by a series of biochemical and histologic modalities, indicate that this imaging system appears to be a promising approach for repeatedly and noninvasively monitoring transplanted hepatic cells," said Zern.

Contact: Dr. Mark A. Zern, Transplant Institute, UC Davis Medical Center, (916) 734-8063; mazern@ucdavis.edu


Using small particles of iron oxide

A second study involving imaging published in this issue of Cell Transplantation (Vol. 17 No. 8), evaluated the use of cloned mensenchymal stem cells (MSC) labeled with clinically-approved small particles of iron oxide (SPIO) for the treatment of experimental autoimmune encephalomyelitis (EAE). Results revealed an increase in disease severity when using SPIO while a control experiment with unlabeled MSC did not affect disease severity.

"Treatment with SPIO alone did not alter disease course," said study lead author Dr. Richard Schafer of the Institute of Clinical and Experimental Transfusion Medicine at University Hospital, Tubingen, Germany. "Iron deposition was present in the group treated with SPIO-labeled MSC, indicating that in vivo the initially cell surface bound iron detached from the MSC. Our results could be of great importance for imaging patients in the clinical setting, indicating that in vivo application of SPIO-labeled MSC needs to be performed with caution since the cell-derived exposure of iron can lead to disease aggravation."

Contact: Dr. Richard Schafer, University Hospital, Tubingen, Germany + 49-7071-295240 richard.schafer@med.uni-tuebingen.de


Three different labeling methods evaluated

In a study conducted at the Center for Research in Cardiovascular Medicine at the State University of New York (SUNY) Buffalo, researchers experimented with three different labeling methods for tracking transplanted mensenchymal stem cells (MSCs): adenovirus-mediated expression of enhanced green fluorescence protein (EGFP); B-galactosidase (LacZ); and live cell DAPI nuclear staining. Each was used to label porcine bone marrow-derived MSCs in cultures.

"Although MSCs could be efficiently labeled by these methods in cultures, whether the labels could be reliably detected after in vivo implantation, particularly in a large animal model, needed to be examined," said study lead author Dr. Techung Lee of the SUNY Buffalo Department of Biochemistry.

The research team experienced 'inconsistency' when attempting to track EGFP and LacZ-labeled MSCs while the DAPI-labeled cells could be 'stably retained' in the implanted MSCs for at least one month after implantation.

"These labeled MSCs could be detected histologically with little ambiguity," commented Lee.

The researchers concluded that DAPI labeling could be reliably used for MSC tracking in both small and large animal models because the brightly emitted blue fluorescence is exclusively confined to the nucleus, generating a sharper image that was advantageous for stem cell identification.

Contact: Dr. Techung Lee, SUNY Buffalo (716) 829-3106 chunglee@buffalo.edu

"The availability of in vivo monitoring of transplanted cells allows visualization of cell migration and proliferation, adding a critical parameter for safety and efficacy measure," said Cesar Borlongan, Ph.D. at the University of South Florida Health, and associate editor of Cell Transplantation.


'/>"/>

Contact: Dr. Mark A. Zern
mazern@ucdavis.edu
916-734-8063
Cell Transplantation Center of Excellence for Aging and Brain Repair
Source:Eurekalert

Related biology news :

1. UI researchers help to improve carbon measurements in global climate studies
2. MSU researcher studies ties between cholesterol drugs, muscle problems
3. Studies of small water fleas help ecologists understand population dynamics
4. ASU researchers receive NIH awards for studies of malaria and emergent disease
5. Drug-embedded microparticles bolster heart function in animal studies
6. NIAID funds studies of how SARS and bird flu evade antiviral responses
7. 6 environmental research studies reveal critical health risks from plastic
8. NIHs Genes, Environment and Health Initiative adds 6 studies
9. New studies find global warming will have significant economic impacts on Florida coasts
10. Fly studies reveal immune cell responses to tumor and tissue damage
11. New studies on the Mediterranean diet confirm its effectiveness for chronic disease prevention
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/28/2016)... SAN FRANCISCO and BANGALORE, India ... part of EdgeVerve Systems, a product subsidiary of Infosys (NYSE: ... service provider, today announced a global partnership that ... convenient way to use mobile banking and payment services. ... Mobility is a key innovation area for financial services, but ...
(Date:4/26/2016)... and LONDON , ... Finacle, part of EdgeVerve Systems, a product subsidiary ... Onegini today announced a partnership to integrate the ...      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ) ... provide their customers enhanced security to access and ...
(Date:4/19/2016)... 20, 2016 The new GEZE ... compact web-based "all-in-one" system solution for all door components. ... or the door interface with integration authorization management system, ... systems. The minimal dimensions of the access control and ... building installations offer considerable freedom of design with regard ...
Breaking Biology News(10 mins):
(Date:5/18/2016)... Calif. (PRWEB) , ... May 18, 2016 , ... ... awarded the Luis Villalobos Award to Cognition Therapeutics at the annual ACA Summit last ... company that is financed by one of ACA’s member angel groups. It is the ...
(Date:5/18/2016)... , May 18, 2016 The Biotech ... does not mean that there are no opportunities ahead. Today, ... Inc. (NASDAQ: THLD ), Seattle Genetics Inc. (NASDAQ: ... ), and Ophthotech Corp. (NASDAQ: OPHT ). Sign ... at: http://www.activewallst.com/ Threshold Pharmaceuticals ...
(Date:5/18/2016)... ... 18, 2016 , ... Every day, more than 5,400 ER ... Costing more than $56 billion in direct costs annually, asthma remains a critical ... the suffering associated with uncontrolled asthma can be overwhelmingly disproportionate and better managed,” ...
(Date:5/18/2016)... ... ... Ryan Benton was diagnosed with Duchenne Muscular Dystrophy (DMD) at the age ... DMD is a relatively common progressive genetic disorder, which causes aggressive deterioration of the ... He met with the founder of the Stem Cell Institute in Panama ...
Breaking Biology Technology: