Putnam Valley, NY. (Nov. 15, 2012) Two studies published in the current issue of Cell Transplantation (21:7), now freely available on-line at http://www.ingentaconnect.com/content/cog/ct/, investigate the role of oxygen in cell transplantation.
In a study carried out at Baylor University, researchers concerned about the poor efficacy of islet cell transplantation during pancreas preservation and islet isolation have found that low temperatures can prevent hypoxia (low oxygen) that can damage islet cells. In a second study, Brazilian researchers found that neural cells derived from induced pluripotent stem cells generated from skin fibroblasts of a schizophrenic patient show extra oxygen consumption and elevated levels of "reactive oxygen species."
Low temperature prevents hypoxia-induced damage
According to a Baylor University, Texas research team, a major problem in islet cell transplantation is the poor condition of islet cells attributed to hypoxia during cell preservation and isolation.
"Islet cells are highly sensitive to hypoxic conditions," said study co-author Dr. Shinichi Matsumoto of the Baylor Research Institute's Islet Cell Laboratory. "We hypothesized that low temperature conditions could prevent hypoxia induced islet cell damage since cold conditions prevented the release of high levels of high-mobility group box 1 protein (HMGB1)."
Studies related to developing better results are limited, said the researchers, although cold preservation has been applied.
In this study, carried out using mouse models, the research team stored islets at 37oC, 22oC and 4oC. Results showed that the islets stored at 4oC "showed significantly higher insulin secretion" when compared to those stored at 37oC.
"This proved that hypothermic conditions could maintain the islets' ability to secrete insulin, even when exposed to hypoxic damage," sai
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Cell Transplantation Center of Excellence for Aging and Brain Repair