Navigation Links
Structures of important plant viruses determined
Date:10/1/2008

"People have been trying to get this structural work started for decades, more than 40 years. It's been very difficult and there have been a number of obstacles, including the fact that it's very hard to make good samples of these viruses. But even after we were able to do that, and analyze the structures using x-ray diffraction and traditional electron microscopy, there were a lot of ambiguities in the results. Those techniques gave us several answers and we didn't know which was correct."

The STEM technique used at Brookhaven Lab provided the definitive answer. Though by itself, STEM cannot determine the structure of the viral protein coat, it is able to put boundaries on the number of molecules in each "turn" of the spiral-shaped structure. That is, it doesn't tell you the shape of the molecules but it can count them.

"STEM measures the number of electrons scattered from a length of filament, thereby 'weighing' the segment relative to a standard in this case, tobacco mosaic virus," said Brookhaven's Joe Wall. "Knowing the mass per unit length, the subunit size, and the axial repeat gives the number of subunits per turn." According to Stubbs, "The number of molecules per turn in the helix is the key. It allowed us to determine which structure of the alternatives we'd come up with from other techniques was correct."

One surprise was that the two viruses the scientists studied, though from unrelated families, turned out to have very similar structures: thin filaments with a spiral structure featuring just under nine molecular subunits per helical turn. "The spiral is so tightly packed that it could not be seen by conventional electron microscopy," Stubbs said.

Some scientists had predicted there would be similarities between these virus families, but there was a lot of disagreement. This study provides the first experimental evidence that they are indeed similar in shape. That could speed up the pace of discovery because what sc
'/>"/>

Contact: Karen McNulty Walsh
kmcnulty@bnl.gov
631-344-8350
DOE/Brookhaven National Laboratory
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. A step toward tissue-engineered heart structures for children
2. Using nanotubes to detect and repair cracks in aircraft wings, other structures
3. First high-res 3D structures of mammalian HSP90 protein solved
4. Mini subs to probe odd structures in BC lake
5. Researchers study facial structures, brain abnormalities to reveal formula for detection of autism
6. Carbon nanostructures form the future of electronics and optoelectronics
7. Nicotinic receptors may be important targets for treatment of multiple addictions
8. Scientists discover important beauty secret for balanced skin color and tone
9. Fossilized cashew nuts reveal Europe was important route between Africa and South America
10. Researchers discover important tool in understanding differentiation in human embryonic stem cells
11. Lipids in the brain an important factor for Alzheimers disease?
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/14/2014)... JOLLA, CA – October 14, 2014 – Scientists from ... million from the National Institutes of Health (NIH) to ... prevalent virus-induced hemorrhagic fever disease in Africa. The study ... and why some patients die, while others survive the ... to understand the basic mechanism of how Lassa fever ...
(Date:10/14/2014)... team of researchers, led by the Chinese Academy of ... Nature Genetics a brief genomic history of tomato ... tomato plant. , The C.M. Rick Tomato Genetics Resource ... this study by providing seed of both cultivated tomato ... builds on the first tomato genome sequence completed just ...
(Date:10/14/2014)... October 14, 2014 – Over the past decades, we ... institutions to meet the needs of infants for social ... these infants to thrive. , Infancy and ... of the cortex. A generation of research suggests that ... cortical development and cognitive function. In contrast, deprivation and ...
Breaking Biology News(10 mins):Scripps Research Institute team receives $6.6 million to investigate deadly Lassa virus 2Institutional rearing may increase risk attention-deficit disorder 2
... that links global warming to the recent extinction of ... evidence of a large phenomena that may affect broad ... researchers at Oregon State University. , A study being ... that global climate change created favorable conditions for a ...
... Assertive and even aggressive human behavior could explain why ... downward trend in close encounters with the oceanic predators, ... precautions and in-your-face responses to confrontations with sharks went ... attacks from 65 in 2004 to 58 in 2005 ...
... Very thin but hardy, unblemished skin and slow developing ... hatched Antarctic notothenioids, a group of fish whose adults ... in their blood. , Such adaptations are important, researchers ... larval fish of at least two species of notothenioids ...
Cached Biology News:Extinctions linked to climate change 2Extinctions linked to climate change 3World shark attacks dipped in 2005, part of long-term trend 2World shark attacks dipped in 2005, part of long-term trend 3Thin tough skin, slow-growing gills protect larval Antarctic fish 2Thin tough skin, slow-growing gills protect larval Antarctic fish 3
(Date:10/20/2014)... , Oct. 20, 2014 Mapp ... experimental ZMapp™ antibody therapeutic to fight the Ebola ... difficult and time-consuming the production of pharmaceuticals can ... research publisher said that while some may be ... this compound, those with industry knowledge are well ...
(Date:10/20/2014)... /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the Company") today ... to $5.8 million and provides a good start to Q4.  The ... America and one in the Middle East ... record levels," said Peter Bruijns , President & CEO. "Total ... Q3 than they have been for any complete year since the ...
(Date:10/20/2014)... PureTech , a science and technology development ... today the closing of a $55 million growth ... a $120 Billion group of funds. The funding ... forward and to advance new healthcare related products ... really go for the big ideas that can ...
(Date:10/20/2014)... Local veterinary surgeon, Dr. Tim McCarthy is seeking ... stem cells for dogs with osteoarthritis. Dr. McCarthy has ... clinical stem cell therapy for 7 years. The ultimate ... single injection of donor stem cells into one or ... inflammation in the treated joints. , Candidates for ...
Breaking Biology Technology:Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 2Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6Cascade Veterinary Referral Center Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2
... venture capital in more deals in 2006, but the improvement ... Ernst & Young, LLP and Dow Jones ... in 2006, including a $12 .5 million round by ... for a total of $58.47 million. The total, up from ...
... Lance Armstrong is a unique phenomenon. He's a talented athlete, ... league by himself. Or is he? , ,As many people know, ... National Center for Health Statistics report released by the ... the second year in a row. , ,Cancer patients as political ...
... director of technology programs for the University of Wisconsin-Madison, is ... he demonstrates in Part II of this interview. In ... In Part II (below), he addresses creating a culture of ... also is a featured speaker at the Fusion 2007 ...
Cached Biology Technology:Wisconsin fares slightly better in venture capital chase 2Wisconsin fares slightly better in venture capital chase 3Lance Armstrong and the future of cancer care 2Lance Armstrong and the future of cancer care 3Lance Armstrong and the future of cancer care 4Lance Armstrong and the future of cancer care 5
... demands of the routine trace metal analytical ... robust and simple to use instrumentation. The ... most challenging sample matrices found in the ... the toughest analytical challenges found in the ...
... Do you need to make your strain ... efficiency? Take advantage of Lucigen’s unrivalled expertise ... Competent Cell Service offers: Chemically ... efficiencies Fast turnaround ...
Procollagen type I C-terminal propeptide (human, PICP)...
... in Microarrays: Biomedical Photometrics Inc. (Waterloo, Ontario, ... Germany) have collaborated to develop a fluorescence ... The DNAscope AT is based on ... AT automatically reads up to six ArrayTubes ...
Biology Products: