Navigation Links
Structure of bacterial nanowire protein hints at secrets of conduction
Date:11/12/2013

break down minerals in rock, but the system can also be used to clean up toxic heavy metals or to run a bacterial fuel cell.

To better understand how pilins contribute to conduction, Reardon and NMR lead scientist Karl Mueller explored pilin from an electrically conducting bacteria known as Geobacter sulfurreducens.

Previous research on Geobacter's pilin -- PilA -- provided a big hint. PilA required certain spots along its length known as aromatic residues to conduct electricity. Without those aromatic residues where they were, Geobacter had no zip in its pili.

But proteins are like a long string that folds up into a compact three-dimensional shape. Without knowing the shape of pilin, it wasn't clear where the aromatic residues landed in space or how they contributed to electron shuttling.

Hop or Flow?

To find out, the researchers used NMR -- a technology similar to medical MRIs -- at EMSL to picture the shape of PilA.

On its own, PilA looks like a long skinny spring, with a slight kink about halfway up. The aromatic residues, which are bulky anyway, bulge along its length. But the protein by itself isn't enough to reveal how conduction works. Many pilin proteins work together to form a fiber, and Reardon and Mueller only had one.

Nor did the researchers have the whole fiber to put into the NMR instrument. To get more clues, Reardon borrowed the computer image of an assembled fiber from an unrelated species, the bacteria that cause gonorrhea. Gonorrhea's fiber does not conduct electricity nor does its pilin have as many aromatic residues. But its pilin has a similar shape to PilA, so using a computer program, Reardon overlaid PilA on its Gonorrhea cousins.

At this point, the aromatic residues clearly stood out.

"We get clusters of aromatic residues, and they wrap along the wire candy cane style," said Reardon.

But that just raised another question
'/>"/>

Contact: Mary Beckman
mary.beckman@pnnl.gov
509-375-3688
DOE/Pacific Northwest National Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. UMMS researchers answer century old question about 3D structure of mitotic chromosomes
2. Brain structure in post-traumatic stress disorder
3. Next-generation global e-infrastructure for taxon names registry
4. Model virus structure shows why theres no cure for common cold
5. CSHL is part of iPlant group awarded $50 million to create US biology cyberinfrastructure
6. $50 million NSF grant to advance cyberinfrastructure for big data in life sciences
7. Changes to symptom structure in DSM-5 support diagnosis of toddlers with autism spectrum disorder
8. Wistar scientists decipher structure of NatA, an enzyme complex that modifies most human proteins
9. AMP concerned about the structure and application of gap fill payment amounts
10. The genomes 3-D structure shapes how genes are expressed
11. Structure of cell signaling molecule suggests general on-off switch
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Structure of bacterial nanowire protein hints at secrets of conduction
(Date:4/15/2014)... pairs, researchers from the Bloomberg School of Public ... reuptake inhibitors (SSRIs), a frequently prescribed treatment for ... autism spectrum disorder (ASD) and developmental delays (DD) ... edition of Pediatrics , analyzed data from ... population-based controls, where a uniform protocol was implemented ...
(Date:4/15/2014)... who study the deadliest infectious diseases recognized the contributions ... at the University of Texas Medical Branch, with a ... on Filoviruses. The filoviruses include Ebola and Marburg viruses ... people infected. The current outbreak of Ebola virus raging ... so far. , "This award represents the culmination ...
(Date:4/15/2014)... form could help reduce side effects by targeting the ... have developed nanoparticles that deliver one or two chemotherapy ... particles that can carry any more than that in ... a new way to build such nanoparticles, making it ... In a paper published in the Journal of ...
Breaking Biology News(10 mins):Study: SSRI use during pregnancy associated with autism and developmental delays in boys 2International research group recognizes UTMB experts 2International research group recognizes UTMB experts 3Targeting cancer with a triple threat 2Targeting cancer with a triple threat 3
... held its breath when the tanker Jessica, loaded with 150,000 ... in the heart of one of the most precious, famous ... At risk were vast numbers of unique species of flora ... to his landmark theory of evolution by natural selection. ...
... policy-makers, business leaders and environmentalists are joining forces to ... taking part in an influential new forum at the ... February 22, the Nitrous Oxide Focus Group will engage ... Marks & Spencer, British Sugar, Defra, the Country Land ...
... , Imagine a gigantic, inflatable, sausage-like bag capable of ... equivalent of 2.2 days of current global emissions. Now try ... radius and several kilometres long, resting benignly on the seabed ... first blush, this might appear like science fiction, but its ...
Cached Biology News:First wind turbines on Galapagos Islands will halve diesel imports, reduce risk of future oil spills 2First wind turbines on Galapagos Islands will halve diesel imports, reduce risk of future oil spills 3First wind turbines on Galapagos Islands will halve diesel imports, reduce risk of future oil spills 4Nitrous oxide: definitely no laughing matter 2Into the abyss: Deep-sixing carbon 2
(Date:1/15/2014)... 2014 The Microcompetition with Foreign DNA ... disease. One of these latent viruses is the Epstein ... rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic ... by the theory, a study found that RA patients ... (1). , What is Microcompetition? , Dr. Hanan ...
(Date:1/15/2014)... Sunnyvale, CA (PRWEB) January 15, 2014 ... of high throughput research solutions, today announced that ... companies, has received delivery of Freeslate’s CM ... form screening. , Lupin, headquartered in Mumbai, ... quality, affordable generic and branded formulations and Active ...
(Date:1/14/2014)... (PRWEB) January 14, 2014 Date: Friday, ... p.m. , Location: Warrington Country Club, 1360 Almshouse Road, ... Foundation, the only national nonprofit organization solely dedicated to ... quality of life for those affected worldwide, will host ... Warrington Country Club in Warrington, Pa. , Each ...
(Date:1/14/2014)... , Jan. 14, 2014  3D Communications, a leading provider ... major scientific, regulatory, business, and media events in ... , today announced its former associate Virginia Cox , ... office. Virginia Cox re-joins ... Commissioner for the Office of External Affairs at the U.S. ...
Breaking Biology Technology:Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2Former FDA Associate Commissioner Returns To 3D Communications 2
... SAN DIEGO , Feb. 16 BioMed Realty ... of an amendment to its lease with Ironwood Pharmaceuticals, Inc. (Nasdaq: ... in Cambridge, Massachusetts .  Ironwood has agreed to lease an ... its existing premises located in the heart of the Kendall Square ...
... , SAN FRANCISCO , Feb. 16 ... showcase innovative new initiatives at Informex this week, highlighting the ... to the global chemicals industry. , At Informex 2010, ... between seven leading universities, the private sector and the Scottish ...
... PLEASANTON, Calif. , Feb. 16 ... proprietary chemistry for nucleic acid testing applications that are relevant ... for the "Most Promising Company" award at the Personalized Medicine ... The PMWC 2010 was created to examine the emerging field ...
Cached Biology Technology:BioMed Realty Trust Expands Lease With Ironwood Pharmaceuticals for 50,000 Square Feet in Cambridge, Massachusetts 2BioMed Realty Trust Expands Lease With Ironwood Pharmaceuticals for 50,000 Square Feet in Cambridge, Massachusetts 3BioMed Realty Trust Expands Lease With Ironwood Pharmaceuticals for 50,000 Square Feet in Cambridge, Massachusetts 4Scotland Launches Chemicals Industry Partnerships To Propel $13 Billion Sector 2Scotland Launches Chemicals Industry Partnerships To Propel $13 Billion Sector 3Scotland Launches Chemicals Industry Partnerships To Propel $13 Billion Sector 4QuantaLife, Inc. Unanimously Selected as the 'Most Promising Company' at the Personalized Medicine World Conference 2010 2QuantaLife, Inc. Unanimously Selected as the 'Most Promising Company' at the Personalized Medicine World Conference 2010 3QuantaLife, Inc. Unanimously Selected as the 'Most Promising Company' at the Personalized Medicine World Conference 2010 4
... coli containing a clone of the human Topoisomerase ... II alters the topological state of nucleic acids ... transient break which generates a separate DNA helix ... DNA passage mechanism, the enzyme can relax negatively ...
RNase-Free DNase 50 l...
supplied with 10x reaction buffer...
... RNase H degrades, as an endoribonuclease, ... DNA hybrids of natural origin, e.g. ... synthetic complexes, e.g. poly(A) x poly(dT). ... and 3-OH termini. Nearly no activity ...
Biology Products: