Navigation Links
Striking the right balance: JBEI researchers counteract biofuel toxicity in microbes
Date:5/11/2011

d bacterial genomes. They then performed a bioinformatics screen to compare regions predicted to be responsible for substrate specificity to those of TtgB, a well-characterized solvent-resistant efflux pump.

"This metric allowed us to rank the complete set of pumps and select a subset that represented a uniform distribution of candidate genes," says Mukhopadhyay. "To construct the library, we amplified efflux pump operons from the genomic DNA of the selected bacteria, cloned them into a vector, and transformed the vector into an E. coli host strain."

In a series of survival competitions, the two microbial efflux pumps that performed best were the native E. coli pump AcrAB and a previously uncharacterized pump from a marine microbe Alcanivorax borkumensis.

"We focused on the A. borkumensis pump and tested it in a strain of host microbe engineered to produce the limonene jet fuel precursor," Mukhopadhyay says. "Microbes expressing the pump produced significantly more limonene than those with no pump, providing an important proof of principle demonstration that efflux pumps that increase tolerance to exogenous biofuel can also improve the yield of a production host."

Mukhopadhyay and her JBEI colleagues have begun evaluating microbial efflux pumps for other important compounds as well as inhibitors present in the carbon source from lignocellulose. They are also looking to improve the A. borkumensis pump and other high performers in their current library, and to optimize the systems by which pump genes are expressed in engineered biofuel-producing microbial strains.

"We believe our bioprospecting strategy for biofuel tolerance mechanisms is going to be a valuable and widely applicable tool in the biotechnology field for engineering new microbial production strains," Mukhopadhyay says.


'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Supplement produces a striking endurance boost
2. Brains, worms and computer chips have striking similarities
3. Youku Upgrades Fingerprinting System for Tougher Copyright Protection Measures
4. How molecules get to the right place at the right time
5. Youku Upgrades Fingerprinting System for Tougher Copyright Protection Measures
6. Jurassic Park had it right: Some dinosaurs hunted by night
7. Recipe for family mealtimes calls for 3 ingredients in the right amounts
8. Laser welding in the right light
9. Researchers discover giant crayfish species right under their noses
10. The right food supplements during pregnancy?
11. Polar bears no longer on thin ice: researchers say polar bears could face brighter future
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Striking the right balance: JBEI researchers counteract biofuel toxicity in microbes
(Date:8/1/2014)... elements are a key component of phloem, the conductive ... range of signalling molecules. Elongated cylindrical cells are capped ... end-to-end to form sieve tubes which in turn form ... "Sieve elements are very special cells which play an ... little has been known about their differentiation," says Professor ...
(Date:8/1/2014)... Fla. , Aug. 1, 2014  LiveScan Service Provider Binary Biometrics ... owner/operator team. As a result, Binary Biometrics will improve its mobile service capabilities ... Volusia County and service several new cities such as, ... , DeLand , Deltona , ... Helen , New Smyrna Beach , Oak ...
(Date:7/31/2014)... supported by the National Science Foundation (NSF), counters ... accelerates atmospheric warming, indicating instead that certain Arctic ... into the atmosphere. , The study, published ... focuses on thermokarst lakes, which occur as permafrost ... melted fresh water, converting what was previously frozen ...
Breaking Biology News(10 mins):Developmental regulation of important plant phloem components discovered 2Binary Biometrics, Founded by James Timothy White, Expands Services to 13+ Locations With the Addition of Owner/Operator Kevin Morris 2Binary Biometrics, Founded by James Timothy White, Expands Services to 13+ Locations With the Addition of Owner/Operator Kevin Morris 3Certain Arctic lakes store more greenhouse gases than they release 2
... On the first anniversary of the American Recovery and ... nation,s research universities today provided nearly 100 examples of ... paying outsize short- and long-term dividends for the nation. ... $21.5 billion is allocated for research and science infrastructure. ...
... Medical Inc. has obtained a non-exclusive license to ... property developed at Case Western Reserve University,s Department ... (FES) Center in Cleveland. ... manufacturer based in Indiana, Pa., rights to intramuscular ...
... multitude of questions, but sometimes traditional techniques are superior. ... cost, when they relied upon statistical data to monitor ... an indigenous group of people living in the James ... Montreal), have lived in harmony with their environment. They ...
Cached Biology News:Universities highlight benefits of stimulus research funding 2Universities highlight benefits of stimulus research funding 3Listen to the natives for better moose monitoring 2
(Date:7/31/2014)... people over 60 are not donor candidates for bone ... time, making the elderly prone to life-threatening infection and ... now has discovered a reason why. , "We have ... blood-forming cells to maintain blood production over time in ... could be restored for rejuvenation therapies," said Emmanuelle Passegu, ...
(Date:7/31/2014)... Ind. -- Chemists at Indiana University Bloomington have ... bricks-and-mortar fashion, a development with potential value for ... field-effect transistors and photovoltaic cells. , Their paper, ... Crystalline Solids and 2D Self-Assembled Crystals," has been ... journal of the Royal Society of Chemistry. It ...
(Date:7/31/2014)... Graphite Metallizing Corporation announces GRAPHALLOY® ... These bearings are uniquely suited to prevent loss of ... of the high temperatures . , Recently, a ... design engineers at Graphite Metallizing to overcome problems with ... manufacturing operation, pans containing blocks of powder mixture are ...
(Date:7/31/2014)... Francisco, California (PRWEB) July 31, 2014 ... systems that inform therapeutic decision-making in oncology, today announced ... August 14, 2014 to discuss the Company's financial results ... provide an update about the business in the current ... audio call beginning on Thursday, August 14, 2014, at ...
Breaking Biology Technology:Key to aging immune system is discovered 2Chemists demonstrate 'bricks-and-mortar' assembly of new molecular structures 2Chemists demonstrate 'bricks-and-mortar' assembly of new molecular structures 3Graphite Metallizing Announces Bearings for High Temperature Oven Applications 2Graphite Metallizing Announces Bearings for High Temperature Oven Applications 3CollabRx, Inc. Schedules First Quarter Fiscal Year 2015 Financial Conference Call and Business Update 2
... N.C., June 9, 2011 The David H. Murdock ... Immune Tolerance Institute, Inc. (ITI) as a next step ... the North Carolina Research Campus in Kannapolis, NC. Driven ... of breakthrough treatments for the range of immune-related diseases, ...
... all advanced a few steps closer to their future ... the week of May 9-13, 2011. That,s when ... three of the four robotics competitions at the IEEE ... China. The trio of contests are designed to prove ...
... 2011 Ronald W. Davis, PhD, a pioneer in innovative ... and genomic methods to biological organisms, will receive the 2011 ... Davis, 69, has spent most of his professional ... biochemistry and genetics. He also serves as director of the ...
Cached Biology Technology:David H. Murdock Research Institute Assumes Control of the Immune Tolerance Institute and Establishes a State of the Art Human Immune Monitoring Laboratory for Biomarker Discovery and Development 2David H. Murdock Research Institute Assumes Control of the Immune Tolerance Institute and Establishes a State of the Art Human Immune Monitoring Laboratory for Biomarker Discovery and Development 3NIST contests in China put next-gen robot technologies to the test 2NIST contests in China put next-gen robot technologies to the test 3Biochemist and Geneticist Ronald W. Davis Receives $500,000 Gruber Genetics Prize for Pioneering Work in the Development of Biotechnologies that Have Significantly Advanced the Fields of Molecular Genetics and Genomics 2Biochemist and Geneticist Ronald W. Davis Receives $500,000 Gruber Genetics Prize for Pioneering Work in the Development of Biotechnologies that Have Significantly Advanced the Fields of Molecular Genetics and Genomics 3
GlutaMAX-I Supplement...
Chemically Defined Lipid Concentrate...
... membrane-filtered isotonic and enzyme-free aqueous formulations ... agents in either Ca2+- and Mg2+-free ... Ca2+- and Mg2+-free phosphate-buffered saline for ... support substrates and each other for ...
... , 25 g Blocking Reagent , 2 x ... T7 Elution Buffer , 0.2 ml BL21 glycerol stock ... ml BLT5615 glycerol stock , 1010 pfu T7Select Positive ... , 50 l S-protein HRP Conjugate , 2 ...
Biology Products: