Navigation Links
Stretching the truth: JILA biophysicists help unravel DNA stretching mystery
Date:1/20/2011

Using a new experimental test structure, biophysicists at JILA have unraveled part of a 15-year mystery in the mechanics of DNAjust how the molecule manages to suddenly extend to almost twice its normal length. The new test structure should support research on DNA elasticity as a standard for tiny forces and help refine studies of how drugs and other substances bind to DNA.

In a new paper in the Journal of the American Chemical Society,* JILA scientists disprove a leading explanation for DNA overstretching, a curious behavior in which the molecule's double helix structure (see image) suddenly extends by 70 percent when subjected to 65 picoNewtons (pN) of force. The exact steps of the process have been controversial since overstretching was discovered in 1996. Contrary to a popular theory, the new JILA work shows that DNA's backbone does not need to have a small gap, often called a nick, or sport loose ends for the dramatic extension to occur at 65 pN.

JILA is a joint institute of the National Institute of Standards and Technology (NIST) and the University of Colorado at Boulder.

"Overstretching is a really amazing phenomenon, and even after 15 years, people are still debating it," says senior author Tom Perkins, a NIST expert in the mechanics of single molecules. "DNA stretches and stretches like a rubber band and you think it's going to break, and then all of a sudden it nearly doubles in length. Something wonderfully interesting happens at this transition."

The leading theory is that overstretching introduces so much energy that the DNA melts, with a single strand peeling off from nicks in the backbone or free ends. This model assumes that nicks or ends are essential. Scientists did not test this assumption until now because there was no platform for restraining the ends without also locking the molecule's structure and rotation.

The JILA team's key advance was a clever geometry that binds a looped end of DN
'/>"/>

Contact: Laura Ost
laura.ost@nist.gov
303-497-4880
National Institute of Standards and Technology (NIST)
Source:Eurekalert

Page: 1 2

Related biology news :

1. Stretching molecules yields new understanding of electricity
2. Penn biophysicists create new model for protein-cholesterol interactions in brain and muscle tissue
3. Brain structure provides key to unraveling function of bizarre dinosaur crests
4. Gene against bacterial attack unravelled
5. Yale researchers unravel mystery of brain aneurysms
6. Soybean grant gives researchers tools to unravel better bean
7. Chemist receives NIH funding to unravel tricks of neuronal wiring
8. HWI scientist first in world to unravel structure of key breast cancer target enzyme
9. Physics, math provide clues to unraveling cancer
10. Freaks help scientist unravel nature and nurture
11. Study unravels why certain fishes went extinct 65 million years ago
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/22/2014)... 22, 2014  NXT-ID, Inc. (NASDAQ: NXTD and ... on the growing mobile commerce market, announces it has retained ... for the 2015 International CES debut of the Wocket™ biometric ... be at the NXT-ID booth January 6th and 7 th ...
(Date:12/22/2014)... , Dec. 22, 2014  The 2014 Holiday Season ... as Acuity Market Intelligence reports that the long anticipated ... forecasts that intensifying demand for smart phones, tablets, and ... global market of 2.5 billion users with nearly 4.8 ...
(Date:12/19/2014)... Dec. 18, 2014 Research and Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ... Monitor: North America Perimeter Security Systems Market" report to ... The North American perimeter security market is estimated to ... Although the U.S. market holds a larger share in this ...
Breaking Biology News(10 mins):NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4First Season of Holiday Shopping with Mobile Biometric Payments Wraps Up With a Present for Biometrics Industry: A Rosy Forecast for More Than 2 Billion Users of 4.8 Billion Mobile Biometric Devices by 2020 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3
... but some waterfall-climbing fish have adapted to their extreme lifestyle by ... according to research published January 4 in the open access journal ... University. The Nopili rock-climbing goby is known to inch its ... combination of two suckers; one of these is an oral sucker ...
... technology is altering the way cancer is diagnosed and treated, ... That,s the message delivered this week in a column ... ( JAMA ) by Scripps Clinic physicians Eric Topol, ... cardiologist who serves as chief academic officer of Scripps Health ...
... the health of species based on overall biomass, but a ... that it isn,t the total number of individuals that predators ... It,s more than searching for an easy meal, the researchers ... in searching for food with the caloric and nutrient value ...
Cached Biology News:Scripps physicians call for change in cancer tissue handling 2Bering Sea study finds prey density more important to predators than biomass 2Bering Sea study finds prey density more important to predators than biomass 3
(Date:1/22/2015)... 22, 2015 Eckert & Ziegler Vitalea ... (AMS) services to the pharmaceutical and biotech industry, has ... company in the position of Director of Science & ... and valuable experience in drug metabolism and bioanalysis to ...
(Date:1/22/2015)... 2015 Cambridge Semantics, the leading provider of ... that 2014 was a record-breaking year across the board for ... Anzo Smart Data Platform and our Smart Data solutions, our ... diverse data which led to record growth for the company ...
(Date:1/22/2015)... January 22, 2015 zFlo Motion , ... to announce an exciting product from a new partner. ... solutions over the past 15 years, with offerings in the ... their 3D IMU system (inertial measurement unit), iSen, is opening ...
(Date:1/22/2015)... Nueva Jersey , 22 de enero de 2015  El ... abre hoy su llamada a nominaciones para 2015. Este ... ha hecho, o tiene el potencial para hacer, contribuciones ... nominaciones se aceptarán hasta el 15 de marzo de ...
Breaking Biology Technology:Eckert & Ziegler Vitalea Science appoints Mark Seymour, PhD as Director of Science & Technology 2Eckert & Ziegler Vitalea Science appoints Mark Seymour, PhD as Director of Science & Technology 3Cambridge Semantics Announces Record Results for 2014 23D Motion Capture Just Got Easy 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4
... Madison, Wisconsin, won a $20,000 scholarship for a project that ... took 10th place in the Intel Science Talent Search for ... groups." The awards were handed out in Washington, D.C., on ... $100,000 went to a New York student for a device ...
... $750 million biotechnology plan for the state, especially as budget ... track of where the money is coming from and going, ... initiatives funded through public-private partnerships. , ,WTN compiled this review ... has proposed using close to $240 million in new state ...
... magazine has just put out in its March edition ... Its focus is primarily on the environment for formation ... include some biotechnology and medical devices and diagnostics. , ... to know the key factors they used to compute ...
Cached Biology Technology:What $750m means: a review of the state biotech plan 2Midwest states in top 10 small-tech hotspots 2
... is a compact benchtop and stackable ... controller with a large display screen ... speed. Air circulation inside to evenly ... can be easily calibrated from the ...
Anti Autotaxin /ENPP2 , - (Rabbit)...
Mouse polyclonal antibody to TNPO2 - transportin 2 (importin 3, karyopherin beta 2b)...
...
Biology Products: