Navigation Links
Stowers researchers develop whole genome sequencing approach for mutation discovery

The Stowers Institute's Hawley Lab and Molecular Biology Facility have developed a "whole-genome sequencing approach" to mapping mutations in fruit flies. The novel methodology promises to reduce the time and effort required to identify mutations of biological interest. The work was published in the May issue of the journal GENETICS.

The team mapped a fruit-fly mutation caused by the compound ethyl methanesulfonate (EMS) by determining the DNA sequence of the mutant fly's genome. The results provide insight into the mechanism of EMS mutageneseis and into gene conversion events involving balancer chromosomes genetic tools used to prevent genetic recombination between homologous chromosomes during meiosis.

Model organisms like fruit flies are used in research for studying both normal biological processes and human disease. Fruit fly genes can be inserted, deleted or modified, and large numbers of flies can be randomly mutated to generate interesting phenotypes relevant to human disease. Finding the mutated gene responsible for an interesting phenotype is labor intensive and time consuming, and many mutations that cause medically relevant phenotypes are not discovered. The new approach lowers the barrier to finding mutations and greatly accelerates the discovery of genes important for human health.

"This approach will change the way fruit fly genetics is done," said Scott Hawley, Ph.D., Investigator and co-equal senior author on the publication. "Traditional mapping approaches to identify mutations are inefficient procedures. Our whole-genome sequencing approach is fast and cost effective. Among other potential uses, it also carries the potential to pinpoint inheritable molecular characteristics that are controlled by several genes at once."

"The traditional mapping method could take months to years depending on the complexity of the phenotype," said Karen Staehling-Hampton, Ph.D., Managing Director of Molecular Biology and co-equal senior author on the paper. "This advance will allow us to map mutations of interest in just a few weeks. The next-generation sequencing technology used for this project is extremely exciting. It will allow researchers to sequence genomes for a few thousand dollars, a cost unheard of just a few years ago. It will also enable them to take their science in new directions and answer new questions that were not possible with traditional sequencing technology."


Contact: Marie Jennings
Stowers Institute for Medical Research

Related biology news :

1. Stowers Institutes Xie Lab demonstrates dual intrinsic and extrinsic control of stem cell aging
2. Stowers Institutes Hawley Lab identifies factors responsible for restart of meiotic cycle
3. Stowers Proteomics Center devises method for assigning probabilities to human protein interactions
4. Stowers Institute researchers identify gene linked to vertebral defects in patient populations
5. Stowers Institutes Shilatifard Lab identifies new role for factor critical to transcription
6. Stowers Institutes Workman Lab discovers novel histone demethylase protein complex
7. Stowers Institutes Linheng Li Lab expands understanding of bone marrow stem cell niche
8. Stowers Institutes Baumann Lab identifies key step in maturation pathway of telomerase
9. Researchers identify proteins involved in new neurodegenerative syndrome
10. Texas researchers and educators head for Antarctica
11. MGH researchers describe new way to identify, evolve novel enzymes
Post Your Comments:
(Date:10/29/2015)... Mich. , Oct. 29, 2015  Rubicon ... Genomics for U.S. distribution of its DNA library ... kit and Rubicon,s new ThruPLEX Plasma-seq kit. ThruPLEX ... enable the preparation of NGS libraries for liquid ... for diagnostic and prognostic applications in cancer and ...
(Date:10/27/2015)... -- In the present market scenario, security is one ... verticals such as banking, healthcare, defense, electronic gadgets, and ... secure & simplified access control and growing rate of ... bank accounts, misuse of users, , and so on. ... and smartphones are expected to provide potential opportunities for ...
(Date:10/26/2015)... and LAS VEGAS , ... Nok Labs , an innovator in modern authentication and ... today announced the launch of its latest version of ... platform enabling organizations to use standards-based authentication that supports ... Nok S3 Authentication Suite is ideal for organizations deploying ...
Breaking Biology News(10 mins):
(Date:11/30/2015)... -- Champions Oncology, Inc. (CSBR), engaged in the development of ... and use of oncology drugs, today announced that ... at the LD MICRO Investor Conference on Wednesday, December ... conference, held at the Luxe Sunset Bel Air Hotel ... feature 200 small/micro-cap companies and is expected to host ...
(Date:11/30/2015)...  HUYA Bioscience International, the leader in accelerating global ... today announced it has signed a Memorandum of Understanding ... collaboration between KDDF and HUYA with the ultimate goal ... for the global market. China,s ... innovative preclinical and clinical stage compounds. The company advances ...
(Date:11/30/2015)... Germany , November 30, 2015 ... Vienna, Austria to be held December 1-4, ... in Vienna, Austria to be ... owned subsidiary of Vycor Medical, Inc. ("Vycor") (OTCQB: VYCO), announced ... Therapy Suite at the 3rd European Congress of NeuroRehabilitation ...
(Date:11/30/2015)... PETACH TIKVAH, Israel , Nov. 30, 2015 ... ), a leading developer of adult stem cell technologies for ... Therapeutics Ltd., has been awarded an additional grant of approximately ... Chief Scientist (OCS). This grant, the second this year, brings ... to approximately $1.8 million (approximately NIS7 million).  ...
Breaking Biology Technology: