Navigation Links
Stowers Proteomics Center devises method for assigning probabilities to human protein interactions

The Stowers Institutes Proteomics Center has published a novel method of using normalized spectral counts derived from a series of affinity purifications analyzed by mass spectrometry (APMS) to generate a probabilistic measure of the preference of proteins to associate with one another.

The work which allows for the assignment of probabilities not only to the interactions within well-defined protein assemblies, but also to interactions between complexes was posted today to the Web site of the Proceedings of the National Academy of Sciences (PNAS).

Large-scale APMS studies have played important roles in the assembly and analysis of comprehensive protein interaction networks for lower eukaryotes, such as yeast. But the development of such networks for human proteins has been slowed by the high cost and significant technical challenges associated with systematic studies of protein interactions.

The Stowers Institutes Proteomics Center has addressed this challenge by developing a method for building local and focused protein networks. With this computational approach, the probability for two proteins to associate is calculated from the bait-to-prey relationship alone, an improvement over other methods requiring systematic reciprocal bait-prey interactions or co-purification of preys by a third bait.

Previous protein interaction networks built using protein mass spectrometry data were largely based on binary yes/no data, where a protein is present in a sample or it is not, explains Michael Washburn, Ph.D., Director of Proteomics and senior author on the paper. We were interested in quantitative proteomics approaches. We were able to develop a method to generate more information-rich networks, where the preference of two proteins to associate within a defined complex or within a larger network assembly can be estimated using Baysian probabilities. The new approach adds more information to the analysis of protein complexes and networks, since not all proteins interact in the same way.

The work not only provides a significant advancement in proteomic analysis, it also holds promise in facilitating the development of treatments for disease.

By having insight regarding the most probable contacts within a multiprotein complex, we can devise targeted strategies to disrupt specific interactions, said Mihaela Sardiu, Ph.D., Postdoctoral Research Associate and lead author on the paper. This could be useful for developing new drugs for disrupting protein complexes involved in disease.

The Proteomics Center is one of three technology development centers that support research at the Stowers Institute. In addition to collaborating with Stowers Institute independent research teams, the centers pursue cutting-edge solutions to the evolving challenges of basic biomedical research.

The Stowers Institutes Proteomics Center is providing technology solutions that fundamentally change the way that Stowers researchers approach their work, said Robb Krumlauf, Ph.D., Scientific Director. Because of the support provided by our technology development centers, Stowers research teams can approach long-standing problems in new and innovative ways, elevating their research and results.


Contact: Marie Jennings
Stowers Institute for Medical Research

Related biology news :

1. Stowers Institutes Hawley Lab identifies factors responsible for restart of meiotic cycle
2. Stowers Institutes Xie Lab demonstrates dual intrinsic and extrinsic control of stem cell aging
3. Story ideas from Molecular & Cellular Proteomics
4. Manomet Center awarded major NFWF grant to foster shorebird conservation
5. Ireland Cancer Center researchers advance stem cell gene therapy
6. DFG to establish 10 new collaborative research centers
7. Woods Hole Research Center debuts new image mosaic that will strengthen global forest monitoring
8. Monsanto expands sponsorship for Peking-Yale Joint Agrobiotechnology Center
9. NYUs Center for Genomics & Systems Biology receives $4.4 million NSF grant
10. NHGRI funds new Centers for Excellence in Ethical, Legal and Social Implications Research
11. 3 Columbia University Medical Center faculty elected to Institute of Medicine
Post Your Comments:
(Date:6/2/2016)... 2016 Perimeter Surveillance & Detection ... Physical Infrastructure, Support & Other Service  The ... offers comprehensive analysis of the global Border Security ... revenues of $17.98 billion in 2016. Now: ... leader in software and hardware technologies for advanced video ...
(Date:5/20/2016)... , May 20, 2016  VoiceIt is excited ... with VoicePass. By working together, VoiceIt ...  Because VoiceIt and VoicePass take slightly different approaches ... increases both security and usability. ... about this new partnership. "This marketing ...
(Date:5/9/2016)... Elevay is currently known as ... for high net worth professionals seeking travel for work ... world, there is still no substitute for a face-to-face ... your deal with a firm handshake. This is why ... of citizenship via investment programs like those offered by ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... Houston Methodist Willowbrook Hospital has signed ... to serve as their official health care provider. ... will provide sponsorship support, athletic training services, and ... volunteers, athletes and families. "We are ... and to bring Houston Methodist quality services and ...
(Date:6/23/2016)... , June 23, 2016   EpiBiome , a ... $1 million in debt financing from Silicon Valley Bank ... automation and to advance its drug development efforts, as ... facility. "SVB has been an incredible strategic ... services a traditional bank would provide," said Dr. ...
(Date:6/23/2016)... , June 23, 2016 Apellis Pharmaceuticals, ... 1 clinical trials of its complement C3 inhibitor, ... and multiple ascending dose studies designed to assess ... of subcutaneous injection in healthy adult volunteers. ... either as a single dose (ranging from 45 ...
(Date:6/23/2016)... ... June 23, 2016 , ... ClinCapture, the only free ... and will showcase its product’s latest features from June 26 to June 30, ... poster on Disrupting Clinical Trials in The Cloud during the conference. DIA ...
Breaking Biology Technology: