Navigation Links
Stowers Proteomics Center devises method for assigning probabilities to human protein interactions
Date:1/24/2008

The Stowers Institutes Proteomics Center has published a novel method of using normalized spectral counts derived from a series of affinity purifications analyzed by mass spectrometry (APMS) to generate a probabilistic measure of the preference of proteins to associate with one another.

The work which allows for the assignment of probabilities not only to the interactions within well-defined protein assemblies, but also to interactions between complexes was posted today to the Web site of the Proceedings of the National Academy of Sciences (PNAS).

Large-scale APMS studies have played important roles in the assembly and analysis of comprehensive protein interaction networks for lower eukaryotes, such as yeast. But the development of such networks for human proteins has been slowed by the high cost and significant technical challenges associated with systematic studies of protein interactions.

The Stowers Institutes Proteomics Center has addressed this challenge by developing a method for building local and focused protein networks. With this computational approach, the probability for two proteins to associate is calculated from the bait-to-prey relationship alone, an improvement over other methods requiring systematic reciprocal bait-prey interactions or co-purification of preys by a third bait.

Previous protein interaction networks built using protein mass spectrometry data were largely based on binary yes/no data, where a protein is present in a sample or it is not, explains Michael Washburn, Ph.D., Director of Proteomics and senior author on the paper. We were interested in quantitative proteomics approaches. We were able to develop a method to generate more information-rich networks, where the preference of two proteins to associate within a defined complex or within a larger network assembly can be estimated using Baysian probabilities. The new approach adds more information to the analysis of protein complexes
'/>"/>

Contact: Marie Jennings
mfj@stowers-institute.org
816-926-4015
Stowers Institute for Medical Research
Source:Eurekalert

Page: 1 2

Related biology news :

1. Stowers Institutes Hawley Lab identifies factors responsible for restart of meiotic cycle
2. Stowers Institutes Xie Lab demonstrates dual intrinsic and extrinsic control of stem cell aging
3. Story ideas from Molecular & Cellular Proteomics
4. Manomet Center awarded major NFWF grant to foster shorebird conservation
5. Ireland Cancer Center researchers advance stem cell gene therapy
6. DFG to establish 10 new collaborative research centers
7. Woods Hole Research Center debuts new image mosaic that will strengthen global forest monitoring
8. Monsanto expands sponsorship for Peking-Yale Joint Agrobiotechnology Center
9. NYUs Center for Genomics & Systems Biology receives $4.4 million NSF grant
10. NHGRI funds new Centers for Excellence in Ethical, Legal and Social Implications Research
11. 3 Columbia University Medical Center faculty elected to Institute of Medicine
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/20/2014)... University of Tennessee, Knoxville, research finds life can persist ... professor was part of a team that examined waters ... Antarctic ice sheet and found the extreme environment supports ... by Jill Mikucki and her colleagues has implications for ... in the solar system. The findings are published in ...
(Date:8/20/2014)... MA Using population-based screening outcomes of approximately 3 ... including four researchers at the University of Massachusetts Medical ... immunodeficiency (SCID) can be successfully implemented across public health ... published in the Aug. 20 issue of the ... ) showed the rate of SCID in newborns is ...
(Date:8/20/2014)... Ore. -- When are athletes who have suffered concussions ... Oregon study has found that high school athletes who ... 60 days experience a significant regression in their abilities ... The regression, as seen in changes in their balance ... 19 athletes. Ten of the 12 had returned to ...
Breaking Biology News(10 mins):University of Tennessee research uncovers subglacial life beneath Antarctic ice sheet 2Newborn screening expansion offers early diagnosis and treatment to infants with SCID 2Newborn screening expansion offers early diagnosis and treatment to infants with SCID 3Recovery reversal seen in Oregon study of returning concussed athletes 2Recovery reversal seen in Oregon study of returning concussed athletes 3
... bioMETRX, Inc., (OTC,Bulletin Board: BMRX), the leading ... that Frank Santamorena, a world,renowned security expert, ... October 21, 2008., Frank Santamorena brings ... level,security expertise. Frank is a consultant to ...
... A UC Davis researcher known for her honey ... with their Old World counterparts to enhance their positive ... and very productive, and hopefully will confer increased resistance ... Susan Cobey. The Carniolan honey bee ( Apis ...
... From stiff plastic limbs and metal rods to complex ... test dummies of yesteryear are evolving and researchers at Wake ... to life. This new generation of human body ... known in research "dummies" before. The models will contain detailed ...
Cached Biology News:bioMETRX, Inc. Welcomes Residential and Commercial Security Expert to Board of Directors 2bioMETRX, Inc. Welcomes Residential and Commercial Security Expert to Board of Directors 3Building a better bee 2Wake Forest plays integral role in effort to revolutionize vehicle safety 2Wake Forest plays integral role in effort to revolutionize vehicle safety 3
(Date:8/21/2014)... 21, 2014 Ontotext S4 , ... Ontotext . Now the same enterprise hardened ... structured and unstructured data is available to start-ups and ... enterprise technology. Organizations that do not have resources to ... can use S4 since there is no need for ...
(Date:8/20/2014)... inventions licensed to biotechnology firms has revealed early ... these roadblocks, the researchers suggest that better communication ... could lead to faster commercialization down the road. ... made in university laboratories and licensed to biotechnology ... which have a high failure rate. But a ...
(Date:8/20/2014)... Mass. , Aug. 20, 2014  Decision Resources Group finds ... , Russia , India ... $1.6 billion in 2013, roughly equal to the size of ... however, the BRIC market will experience much faster growth as a ... aging population. Other key findings from Decision Resources Group,s ...
(Date:8/20/2014)... improve the health of your heart, researchers have discovered. ... a standard TENS machine like those designed to relieve ... the small raised flap at the front of the ... The stimulation changed the influence of the nervous system ... can drive failing hearts too hard. , Professor Jim ...
Breaking Biology Technology:Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 2Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 3Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 4Early bottlenecks in developing biopharmaceutical products delay commercialization 2Early bottlenecks in developing biopharmaceutical products delay commercialization 3BRIC Vascular Access Device Market Valued at $1.6 Billion in 2013, Similar to the U.S. Market 2BRIC Vascular Access Device Market Valued at $1.6 Billion in 2013, Similar to the U.S. Market 3'Tickling' your ear could be good for your heart 2
... , ... , , , ... samples of low cell numbers , , , ... cells have, for example,limited availability, quantity,and lifespan. Using the Agilent,2100 bioanalyzer ...
... , , , ... , , , , ... Agilent 2100 bioanalyzer can,be used to analyze apoptotic cell samples. Performance ... compared to,those obtained with a conventional flow cytometer. Histogram quality,of the ...
... , , , , ... The In Situ Cell Death Detection Kit has been designed as a precise, fast, and simple, ... the single-cell level in tissues. It is based on the so-called TUNEL, ... , , , , ...
Cached Biology Technology:Flow cytometric analysis of human,primary cells using the Agilent 2100,bioanalyzer and on-chip staining 2Flow cytometric analysis of human,primary cells using the Agilent 2100,bioanalyzer and on-chip staining 3Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 2Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 3Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 4Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 5Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 6Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 7Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 8Apoptosis detection by annexin V and,active caspase-3 with the Agilent 2100,bioanalyzer 9In Situ Cell Death Detection Kit 2
Rabbit polyclonal to Phosphothreonine-Proline / Phosphoserine-Proline ( Abpromise for all tested applications)....
Mouse monoclonal [7C6] to Bcr-abl ( Abpromise for all tested applications). SwissProtID: P11274...
Chicken polyclonal to Thioredoxin domain containing 9 ( Abpromise for all tested applications). Antigen: Full length protein (Human) Entrez Gene ID: 10190 Swiss Protein ID: O14530...
Mouse anti-IkBa/MAD-3 Class: Antibody Product Group: Signalling molecules and phospho-specific Antibody...
Biology Products: