Navigation Links
Stowers Proteomics Center devises method for assigning probabilities to human protein interactions
Date:1/24/2008

The Stowers Institutes Proteomics Center has published a novel method of using normalized spectral counts derived from a series of affinity purifications analyzed by mass spectrometry (APMS) to generate a probabilistic measure of the preference of proteins to associate with one another.

The work which allows for the assignment of probabilities not only to the interactions within well-defined protein assemblies, but also to interactions between complexes was posted today to the Web site of the Proceedings of the National Academy of Sciences (PNAS).

Large-scale APMS studies have played important roles in the assembly and analysis of comprehensive protein interaction networks for lower eukaryotes, such as yeast. But the development of such networks for human proteins has been slowed by the high cost and significant technical challenges associated with systematic studies of protein interactions.

The Stowers Institutes Proteomics Center has addressed this challenge by developing a method for building local and focused protein networks. With this computational approach, the probability for two proteins to associate is calculated from the bait-to-prey relationship alone, an improvement over other methods requiring systematic reciprocal bait-prey interactions or co-purification of preys by a third bait.

Previous protein interaction networks built using protein mass spectrometry data were largely based on binary yes/no data, where a protein is present in a sample or it is not, explains Michael Washburn, Ph.D., Director of Proteomics and senior author on the paper. We were interested in quantitative proteomics approaches. We were able to develop a method to generate more information-rich networks, where the preference of two proteins to associate within a defined complex or within a larger network assembly can be estimated using Baysian probabilities. The new approach adds more information to the analysis of protein complexes and networks, since not all proteins interact in the same way.

The work not only provides a significant advancement in proteomic analysis, it also holds promise in facilitating the development of treatments for disease.

By having insight regarding the most probable contacts within a multiprotein complex, we can devise targeted strategies to disrupt specific interactions, said Mihaela Sardiu, Ph.D., Postdoctoral Research Associate and lead author on the paper. This could be useful for developing new drugs for disrupting protein complexes involved in disease.

The Proteomics Center is one of three technology development centers that support research at the Stowers Institute. In addition to collaborating with Stowers Institute independent research teams, the centers pursue cutting-edge solutions to the evolving challenges of basic biomedical research.

The Stowers Institutes Proteomics Center is providing technology solutions that fundamentally change the way that Stowers researchers approach their work, said Robb Krumlauf, Ph.D., Scientific Director. Because of the support provided by our technology development centers, Stowers research teams can approach long-standing problems in new and innovative ways, elevating their research and results.


'/>"/>

Contact: Marie Jennings
mfj@stowers-institute.org
816-926-4015
Stowers Institute for Medical Research
Source:Eurekalert

Related biology news :

1. Stowers Institutes Hawley Lab identifies factors responsible for restart of meiotic cycle
2. Stowers Institutes Xie Lab demonstrates dual intrinsic and extrinsic control of stem cell aging
3. Story ideas from Molecular & Cellular Proteomics
4. Manomet Center awarded major NFWF grant to foster shorebird conservation
5. Ireland Cancer Center researchers advance stem cell gene therapy
6. DFG to establish 10 new collaborative research centers
7. Woods Hole Research Center debuts new image mosaic that will strengthen global forest monitoring
8. Monsanto expands sponsorship for Peking-Yale Joint Agrobiotechnology Center
9. NYUs Center for Genomics & Systems Biology receives $4.4 million NSF grant
10. NHGRI funds new Centers for Excellence in Ethical, Legal and Social Implications Research
11. 3 Columbia University Medical Center faculty elected to Institute of Medicine
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/7/2017)... 2017 Brandwatch , the leading social intelligence company, ... Trust to uncover insights to support its reporting, help direct ... The UK,s leading youth charity will be using Brandwatch Analytics social ... a better understanding of the topics and issues that are a ... ...
(Date:3/2/2017)... YORK , March 2, 2017 Summary ... better understand Perrigo and its partnering interests and activities since ... ... Partnering Deals and Alliance since 2010 report provides an in-depth ... leading life sciences companies. On demand company reports ...
(Date:3/2/2017)... March 2, 2017 Australian stem cell and ... CYP), has signed an agreement with the Monash Lung ... Biomedicine Discovery Institute and Department of Pharmacology at Monash ... further preclinical study to support the use of Cymerus™ ... Asthma is a chronic, long term ...
Breaking Biology News(10 mins):
(Date:3/29/2017)... ... March 29, 2017 , ... ComplianceOnline, the leading governance, risk ... its 3rd Annual Medical Device Summit 2017 venue and speaker lineup. The Summit will ... in Boston, MA. , The Omni Parker House Hotel, which is located at 60 ...
(Date:3/29/2017)... , March 29, 2017  Halozyme Therapeutics, ... oncology and drug-delivery therapies, today announced that an ... and Drug Administration voted 11 to 0 that ... skin) injection was favorable for patients in the ... lymphoma and chronic lymphocytic leukemia. The FDA action ...
(Date:3/29/2017)... ... March 29, 2017 , ... ACEA Biosciences, a privately owned ... travel award to noteworthy scientists who will be presenting research using ACEA Biosciences’ ... of awards are being given to two postdoctoral fellows studying pathogen capture by ...
(Date:3/29/2017)... ... March 29, 2017 , ... Bactana Animal Health, a company developing natural products ... through enhancement of the gut microbiota, today announced the closing of its first round ... York-based Sustainable Income Capital Management, LLC and a number of private investors. The company ...
Breaking Biology Technology: