Navigation Links
Stem cells may prevent post-injury arthritis
Date:8/10/2012

DURHAM, N.C.-- Duke researchers may have found a promising stem cell therapy for preventing osteoarthritis after a joint injury.

Injuring a joint greatly raises the odds of getting a form of osteoarthritis called post-traumatic arthritis, or PTA. There are no therapies yet that modify or slow the progression of arthritis after injury.

Researchers at Duke University Health System have found a very promising therapeutic approach to PTA using a type of stem cell, called mesenchymal stem cells (MSCs), in mice with fractures that typically would lead to them developing arthritis. Their findings could lead to a therapy that would be used after joint injury and before signs of significant osteoarthritis.

The scientists thought the stem cells would work to prevent PTA by altering the balance of inflammation and regeneration in knee joints, because these stem cells have beneficial properties in other regions of the body.

"The stem cells were able to prevent post-traumatic arthritis," said Farshid Guilak, Ph.D., director of orthopaedic research at Duke and senior author of the study.

The study was published on August 10 in Cell Transplantation.

The researchers also thought that a type of mice bred for their super-healing properties would probably fare better than typical mice, but they were wrong.

"We decided to investigate two therapies for the study, said lead author Brian Diekman, Ph.D., a postdoctoral researcher in the Guilak lab. "We thought that stem cells from so-called superhealer mice would be superior at providing protection, and instead, we found that they were no better than stem cells from typical mice. We thought that maybe it would take stem cells from superhealers to gain an effect as strong as preventing arthritis after a fracture, but we were surprised and excited to learn that regular stem cells work just as well."

Certain people appear to fall into the superhealer category, too. They bounce back quickly and heal well naturally after a fracture, while other people eventually form cases of arthritis at the fractured joint, said Guilak, who is a professor of orthopaedic surgery and biomedical engineering.

"The ability of the superhealer mice to have superior healing after a fracture may go beyond the properties of their stem cells and be some beneficial factor, like a growth factor, that we don't know about yet," Guilak said.

The delivery of 10,000 typical or superhealer stem cells to the joint prevented the mice from developing PTA, unlike a control group that received only saline.

Diekman said the team looked at markers of inflammation and saw that the stem cells affected the inflammatory environment of the joint after fracture.

"The stem cells changed the levels of certain immune factors, called cytokines, and altered the bone healing response," said Diekman, who is also with the Duke Department of Biomedical Engineering.

Guilak said that very few studies have purified stem cells to the degree they were purified for this study. They used mesenchymal stem cells, which are bone marrow cells not destined to become part of blood.

Diekman said that one of the challenges in the field is isolating and developing a system for sorting the specific cells they wanted, the mesenchymal stem cells, which form a very rare cell type in the bone marrow.

"We found that by placing the stem cells into low-oxygen conditions, they would grow more rapidly in culture so that we could deliver enough of them to make a difference therapeutically," Diekman said.


'/>"/>
Contact: Mary Jane Gore
mary.gore@duke.edu
919-660-1309
Duke University Medical Center
Source:Eurekalert

Related biology news :

1. Anti-aging elixir for solar cells
2. Embryonic blood vessels that make blood stem cells can also make beating heart muscles
3. Long-distance distress signal from periphery of injured nerve cells begins with locally made protein
4. Cell receptor has proclivity for T helper 9 cells, airway inflammation
5. Key function of protein discovered for obtaining blood stem cells as source for transplants
6. Breakthrough technology focuses in on disease traits of single cells
7. New genomic sequencing method enables smarter anaysis of individual cells
8. Blood vessel forming potential of stem cells from human placenta and umbilical cord blood
9. Researchers identify mechanisms that allow embryonic stem cells to become any cell in the human body
10. Nanoscale scaffolds and stem cells show promise in cartilage repair
11. Force of nature: Defining the mechanical mechanisms in living cells
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/2/2016)... , Feb. 2, 2016   Parabon ... from the U.S. Army Research Office and the ... range and sensitivity of the company,s Snapshot ... Accounting Mission and, more generally, defense-related DNA forensics. ... phenotyping capabilities (predicting appearance and ancestry from DNA ...
(Date:2/1/2016)... , Feb. 1, 2016  Wocket® smart wallet ( www.wocketwallet.com ) announces ... personality, Joey Fatone . Las Vegas , ... --> Las Vegas , where Joey appeared ... The new video ad was filmed at the Consumer Electronics Show ... the Wocket booth to meet and greet fans. ...
(Date:1/25/2016)...  Glencoe Software, the world-leading supplier of image data ... provide the data management solution OMERO Plus for the ... Photo - http://photos.prnewswire.com/prnh/20160125/325328LOGO ... Phenotypic analysis measures the characteristics and behavior of ... states such as health and disease, the presence or ...
Breaking Biology News(10 mins):
(Date:2/12/2016)... ... 12, 2016 , ... The Pittcon 2016 Exposition, which takes ... include 848 exhibitors (count as of February 9) of which 119 are first ... by the scientific community in industrial, academic, and government labs. The Exposition will ...
(Date:2/12/2016)... 12, 2016  PTC Therapeutics, Inc. (NASDAQ: ... (Strategies to Realize Innovation, Vision and Empowerment) grant ... provides funds to patient advocacy organizations to develop ... contributions to the rare disease community by increasing ... patient advocates. Mary Frances Harmon ...
(Date:2/11/2016)... , February 11, 2016 Non-profit Consortium ... Genetic Understanding to Support Research and Discovery ... today announced an ambitious plan to sequence 100,000 individuals. It ... countries and at least 7 of North and East Asian ... the first phase, the project will focus on creating phased ...
(Date:2/11/2016)... 2016  Vermillion, Inc. (NASDAQ: VRML ), a ... the formation of the Steering Committee for its Pelvic ... --> Pelvic masses can present physicians and healthcare ... pregnancy is ruled out, pelvic masses may include cancers ... benign ovarian tumors and gastrointestinal and urinary tract masses. ...
Breaking Biology Technology: