Stem-cell transplantation improves muscles in MD animal model, UT Southwestern research...(hich is involved in creating muscle cell...)
hich is involved in creating muscle cells, and then injected those cells into the animals muscles. Those cells caused tumors containing many different types of cells, indicating that there were still residual undifferentiated embryonic stem cells in the cultures at the time of implantation.
Even if there are 10 undesirable cells, thats too many, Dr. Perlingeiro said.
The researchers then began using fluorescent dyes to sort cells depending on whether some surface markers were turned on while others were turned off. By analogy, it was as if they were dealing with a crowd of people and wanted to pull out only those with red hair, green scarves and blue coats, while those with red hair, green scarves and no coats would be disqualified.
The final selection of cells, containing only one type, was again injected into the animals hind-limb muscles. After a month, the fluorescent dyes showed that the cells had deeply penetrated the muscle, an indication that they were growing and reproducing as desired, and many of the muscle fibers also contained dystrophin, the key protein lacking in muscular dystrophy.
After three months, the mice also showed no signs of tumors.
Tests of isolated muscles showed that the treated muscles were significantly stronger than untreated mice lacking dystrophin, although not quite as strong as those of normal mice.
The treated mice also were tested for coordination. Again, their performance was better than that of untreated mice, but not as good as that of normal mice.
The improved coordination is significant because it shows the embryonic stem cells have benefited the animals quality of life, not simply caused an isolated growth with no overall improvement, Dr. Perlingeiro said.
The researchers will next investigate whether these transplanted cells can make muscle stem cells, which are partially developed cells in muscle tissue that serve as a reserve to replenish muscles
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| Contact: Aline McKenzie aline.mckenzie@utsouthwestern.edu 214-648-3404 UT Southwestern Medical Center Source:Eurekalert |
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 Stem-cell transplantation improves muscles in MD animal model, UT Southwestern researchers report |
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