Navigation Links
Stem-cell transplantation improves muscles in MD animal model, UT Southwestern researchers report
Date:1/20/2008

DALLAS Jan. 20, 2008 Using embryonic stem cells from mice, UT Southwestern Medical Center researchers have prompted the growth of healthy and more importantly, functioning muscle cells in mice afflicted with a human model of Duchenne muscular dystrophy.

The study represents the first time transplanted embryonic stem cells have been shown to restore function to defective muscles in a model of muscular dystrophy.

The researchers newly developed technique, which involves stringent sorting to preserve all stem cells destined to become muscle, avoids the risk of tumor formation while improving the overall muscle strength and coordination of the mice, the researchers found.

The mice used in the study lacked dystrophin, the same protein that humans with the fatal wasting disease also are missing.

The study, headed by Dr. Rita Perlingeiro, assistant professor of developmental biology and molecular biology, is available online today and in the February issue of Nature Medicine.

We envision eventually developing a stem-cell therapy for humans with muscular dystrophy, if we are able to successfully combine this approach with the technology now available to make human embryonic stem cells from reprogrammed skin cells, Dr. Perlingeiro said. These cells can be transplanted into the muscle, and they cause muscle regeneration resulting in stronger contractility.

The study represents a major step in the field, she said, because the researchers were able to tease out exactly the cells they wanted.

The problem had been that embryonic stem cells make everything, Dr. Perlingeiro said. They make a great variety of cells. The trick is to pull out only the one type you want.

The UT Southwestern researchers focused on manipulating genes that are active in the very early stages as embryonic stem cells start to develop into more specialized cells. At first, they activated a gene called Pax3, which is involved in creating muscle cells, and then injected those cells into the animals muscles. Those cells caused tumors containing many different types of cells, indicating that there were still residual undifferentiated embryonic stem cells in the cultures at the time of implantation.

Even if there are 10 undesirable cells, thats too many, Dr. Perlingeiro said.

The researchers then began using fluorescent dyes to sort cells depending on whether some surface markers were turned on while others were turned off. By analogy, it was as if they were dealing with a crowd of people and wanted to pull out only those with red hair, green scarves and blue coats, while those with red hair, green scarves and no coats would be disqualified.

The final selection of cells, containing only one type, was again injected into the animals hind-limb muscles. After a month, the fluorescent dyes showed that the cells had deeply penetrated the muscle, an indication that they were growing and reproducing as desired, and many of the muscle fibers also contained dystrophin, the key protein lacking in muscular dystrophy.

After three months, the mice also showed no signs of tumors.

Tests of isolated muscles showed that the treated muscles were significantly stronger than untreated mice lacking dystrophin, although not quite as strong as those of normal mice.

The treated mice also were tested for coordination. Again, their performance was better than that of untreated mice, but not as good as that of normal mice.

The improved coordination is significant because it shows the embryonic stem cells have benefited the animals quality of life, not simply caused an isolated growth with no overall improvement, Dr. Perlingeiro said.

The researchers will next investigate whether these transplanted cells can make muscle stem cells, which are partially developed cells in muscle tissue that serve as a reserve to replenish muscles. They also are testing their implantation approach in animal models of other types of muscular dystrophy.


'/>"/>

Contact: Aline McKenzie
aline.mckenzie@utsouthwestern.edu
214-648-3404
UT Southwestern Medical Center
Source:Eurekalert  

Related biology news :

1. Reprogramming the debate: stem-cell finding alters ethical controversy
2. Clearance of hepatitis C viral infection after liver transplantation
3. Exercise improves thinking, reduces diabetes risk in overweight children
4. Endobronchial valve significantly improves emphysema
5. Teamwork improves learning and career success
6. New clinical data shows chromium picolinate improves cognitive function
7. How molecular muscles help cells divide
8. Small animal imaging facility is big boon to research
9. Report: African, Asian, Latin American farm animals face extinction
10. UT researcher sheds new light on hybrid animals
11. Penn Veterinary Medicine report new strategy to create genetically-modified animals
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Stem-cell transplantation improves muscles in MD animal model, UT Southwestern researchers report
(Date:8/15/2017)...   ivWatch LLC , a medical device company focused on ... receipt of its ISO 13485 Certification, the global standard for medical ... Standardization (ISO®). ... Continuous Monitoring device for the early detection of IV infiltrations. ... "This is an important milestone for ivWatch, as ...
(Date:5/23/2017)... 2017  Hunova, the first robotic gym for the rehabilitation and functional ... in Genoa, Italy . The first 30 robots will ... USA . The technology was developed and patented at ... IIT spin-off Movendo Technology thanks to a 10 million euro investment from ... click: ...
(Date:4/19/2017)... 19, 2017 The global military ... is marked by the presence of several large global ... by five major players - 3M Cogent, NEC Corporation, ... for nearly 61% of the global military biometric market ... the global military biometrics market boast global presence, which ...
Breaking Biology News(10 mins):
(Date:9/22/2017)... ... September 22, 2017 , ... The Academy ... Colorado October 28 and 29, 2017, to promote AMA’s programs, member services, and ... in different hobbies, including but not limited to model aviation and other RC ...
(Date:9/21/2017)... ... September 21, 2017 , ... Dr. Greg Leyer, Chief Scientific ... on September 27th. His presentation is at 12:10pm in the Probiotics Resource Center, Mandalay ... at SupplySide West and discuss how probiotics have shown impressive data in areas outside ...
(Date:9/21/2017)... UK (PRWEB) , ... September 21, 2017 , ... ... a QuintilesIMS company, who enable the world’s most progressive pharma and biotech organizations ... clinical trials at top pharma and biotech events in Q4. , DrugDev will ...
(Date:9/21/2017)... ... September 21, 2017 , ... Lajollacooks4u welcomed the San ... The organization, a worldwide society of professional women with high achievement in the fields ... annual dinner. , Twelve members began with an olive oil tasting to ...
Breaking Biology Technology: