Navigation Links
Stem cell proliferation and differentiation observed within hydrogel
Date:4/11/2013

Stem cells can be coaxed to grow into new bone or new cartilage better and faster when given the right molecular cues and room inside a water-loving gel, researchers at Case Western Reserve University show.

By creating a three-dimensional checkerboardone with alternating highly connected and less connected spaces within the hydrogelthe team found adjusting the size of the micropattern could affect stem cell behaviors, such as proliferation and differentiation.

Inducing how and where stem cells growand into the right kind of cell in three dimensionshas proven a challenge to creating useful stem cell therapies. This technique holds promise for studying how physical, chemical and other influences affect cell behavior in three-dimensions, and, ultimately, as a method to grow tissues for regenerative medicine applications.

"We think that control over local biomaterial properties may allow us to guide the formation of complex tissues," said Eben Alsberg, an associate professor of Biomedical Engineering at Case Western Reserve. "With this system, we can regulate cell proliferation and cell-specific differentiation into, for example, bone-like or cartilage-like cells."

Oju Jeon, PhD, a postdoctoral researcher in Biomedical Engineering, pursued this work with Alsberg. Their work is described April 11, 2013 in the online edition of Advanced Functional Materials.

Hydrogels are hydrophilic three-dimensional networks of water-soluble polymers bonded, or crosslinked, to one another. Crosslinks increase rigidity and alter the porous structure inside the gel.

Alsberg and Jeon used a hydrogel of oxidized methacrylated alginate and an 8-arm poly(ethylene glycol) amine. A chemical reaction between the alginate and the poly(ethylene glycol) creates crosslinks that provide structure within the gel.

They tweaked the mix so that a second set of crosslinks forms when exposed to light. They used checkerboard masks to create patterns of alternating singly and doubly crosslinked spaces.

The spaces, which varied in size at 25, 50, 100 and 200 micrometers across, were evenly singly and doubly crosslinked.

Human stem cells isolated from fat tissue were encapsulated in the singly and doubly crosslinked regions. The doubly-crosslinked spaces are comparatively cluttered with structures. The cells grew into clusters in the singly-crosslinked regions, but remained mostly isolated in the doubly crosslinked regions.

The larger the spaces in the checkerboard, the larger the clusters grew.

Cells were cultivated in media that promote differentiation into either bone or cartilage.

In both the singly and doubly crosslinked spaces, stem cells increasingly differentiated according to the media composition as the space size increased. The results were more dramatic in the singly-crosslinked spaces.

"Potentially, what's happening is the single-crosslinked regions allow better nutrient transport and provide more space for cells to interact and, because it's less restrictive, there's space for new cells and matrix production," Alsberg said. "Cluster formation, in turn, may influence proliferation and differentiation. Differences in mechanical properties between regions likely also regulate the cell behaviors."

The researchers are continuing to use micropatterning to understand the influences of biomaterials on stem cell fate decisions. This approach may permit local control over cell behavior and, ultimately, allow the engineering of complex tissues comprised of multiple cell types using a single stem cell source.


'/>"/>

Contact: Kevin Mayhood
kevin.mayhood@case.edu
216-368-4442
Case Western Reserve University
Source:Eurekalert

Related biology news :

1. Successful stem cell differentiation requires DNA compaction, study finds
2. The first ant methylomes uncover the relationship between DNA methylation and caste differentiation
3. Warmest spring on record causes earliest flowering ever observed in eastern U.S.
4. Boston Healthcare Associates Roundtable Explores Challenges and Opportunities Surrounding the Value of Big Data within the Life Sciences
5. New study suggests that Arabica coffee could be extinct in the wild within 70 years
6. New type of bacterial protection found within cells
7. One law to rule them all -- sizes within a species appear to follow a universal distribution
8. Morphing DNA hydrogel flows like liquid but remembers its original shape
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/23/2017)... -- Hunova, the first robotic gym for the rehabilitation and functional motor sense ... Genoa, Italy . The first 30 robots will be available ... USA . The technology was developed and patented at the IIT ... Movendo Technology thanks to a 10 million euro investment from entrepreneur Sergio ... ...
(Date:5/6/2017)... -- RAM Group , Singaporean based technology ... biometric authentication based on a novel  quantum-state ... perform biometric authentication. These new sensors are based on a ... Group and its partners. This sensor will have widespread ... security. Ram Group is a next generation sensor ...
(Date:4/18/2017)... SUNNYVALE, Calif. , April 18, 2017  Socionext Inc., a ... prototype of a media edge server, the M820, which features the ... face recognition software provided by Tera Probe, Inc., will be showcased ... and at the NAB show at the Las Vegas ... ...
Breaking Biology News(10 mins):
(Date:8/21/2017)... Linda, Ca (PRWEB) , ... August 21, 2017 ... ... has arranged for two speakers for this two-part educational webinar, in which attendees ... complex subunit, and associated protein factor composition. Along with an overview of the ...
(Date:8/18/2017)... ... 2017 , ... Producers of the award winning American Farmer ... upcoming episode, scheduled to broadcast fourth quarter 2017. American Farmer airs Tuesdays at ... family-owned seed company. Educating audiences about its broad portfolio of products to help ...
(Date:8/17/2017)... Springs, FL (PRWEB) , ... August 17, 2017 ... ... announced that the stock market news outlet had provided a research update on ... company's nasally administered TRT product. , According to Soulstring, prescription rates for Natesto® ...
(Date:8/16/2017)... ... August 16, 2017 , ... Tunnell Consulting announced today that ... ISPE Annual Meeting and Expo , to be held October 29 through November ... is “Driving innovation to advance patient therapies.” , The ISPE Annual Meeting and Expo ...
Breaking Biology Technology: