Navigation Links
Stem cell proliferation and differentiation observed within hydrogel
Date:4/11/2013

Stem cells can be coaxed to grow into new bone or new cartilage better and faster when given the right molecular cues and room inside a water-loving gel, researchers at Case Western Reserve University show.

By creating a three-dimensional checkerboardone with alternating highly connected and less connected spaces within the hydrogelthe team found adjusting the size of the micropattern could affect stem cell behaviors, such as proliferation and differentiation.

Inducing how and where stem cells growand into the right kind of cell in three dimensionshas proven a challenge to creating useful stem cell therapies. This technique holds promise for studying how physical, chemical and other influences affect cell behavior in three-dimensions, and, ultimately, as a method to grow tissues for regenerative medicine applications.

"We think that control over local biomaterial properties may allow us to guide the formation of complex tissues," said Eben Alsberg, an associate professor of Biomedical Engineering at Case Western Reserve. "With this system, we can regulate cell proliferation and cell-specific differentiation into, for example, bone-like or cartilage-like cells."

Oju Jeon, PhD, a postdoctoral researcher in Biomedical Engineering, pursued this work with Alsberg. Their work is described April 11, 2013 in the online edition of Advanced Functional Materials.

Hydrogels are hydrophilic three-dimensional networks of water-soluble polymers bonded, or crosslinked, to one another. Crosslinks increase rigidity and alter the porous structure inside the gel.

Alsberg and Jeon used a hydrogel of oxidized methacrylated alginate and an 8-arm poly(ethylene glycol) amine. A chemical reaction between the alginate and the poly(ethylene glycol) creates crosslinks that provide structure within the gel.

They tweaked the mix so that a second set of crosslinks forms when exposed to light. They used checkerboard masks to create patterns of alternating singly and doubly crosslinked spaces.

The spaces, which varied in size at 25, 50, 100 and 200 micrometers across, were evenly singly and doubly crosslinked.

Human stem cells isolated from fat tissue were encapsulated in the singly and doubly crosslinked regions. The doubly-crosslinked spaces are comparatively cluttered with structures. The cells grew into clusters in the singly-crosslinked regions, but remained mostly isolated in the doubly crosslinked regions.

The larger the spaces in the checkerboard, the larger the clusters grew.

Cells were cultivated in media that promote differentiation into either bone or cartilage.

In both the singly and doubly crosslinked spaces, stem cells increasingly differentiated according to the media composition as the space size increased. The results were more dramatic in the singly-crosslinked spaces.

"Potentially, what's happening is the single-crosslinked regions allow better nutrient transport and provide more space for cells to interact and, because it's less restrictive, there's space for new cells and matrix production," Alsberg said. "Cluster formation, in turn, may influence proliferation and differentiation. Differences in mechanical properties between regions likely also regulate the cell behaviors."

The researchers are continuing to use micropatterning to understand the influences of biomaterials on stem cell fate decisions. This approach may permit local control over cell behavior and, ultimately, allow the engineering of complex tissues comprised of multiple cell types using a single stem cell source.


'/>"/>

Contact: Kevin Mayhood
kevin.mayhood@case.edu
216-368-4442
Case Western Reserve University
Source:Eurekalert

Related biology news :

1. Successful stem cell differentiation requires DNA compaction, study finds
2. The first ant methylomes uncover the relationship between DNA methylation and caste differentiation
3. Warmest spring on record causes earliest flowering ever observed in eastern U.S.
4. Boston Healthcare Associates Roundtable Explores Challenges and Opportunities Surrounding the Value of Big Data within the Life Sciences
5. New study suggests that Arabica coffee could be extinct in the wild within 70 years
6. New type of bacterial protection found within cells
7. One law to rule them all -- sizes within a species appear to follow a universal distribution
8. Morphing DNA hydrogel flows like liquid but remembers its original shape
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/22/2016)... ANGELES , June 22, 2016 /PRNewswire/ ... identity management and verification solutions, has partnered ... edge software solutions for Visitor Management, Self-Service ... provides products that add functional enhancements ... partnership provides corporations and venues with an ...
(Date:6/16/2016)... , June 16, 2016 ... size is expected to reach USD 1.83 billion ... Grand View Research, Inc. Technological proliferation and increasing ... applications are expected to drive the market growth. ... , The development of advanced multimodal ...
(Date:6/7/2016)...  Syngrafii Inc. and San Antonio Credit Union ... integrating Syngrafii,s patented LongPen™ eSignature "Wet" solution into ... result in greater convenience for SACU members and ... existing document workflow and compliance requirements. ... Highlights: ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... -- Liquid Biotech USA , Inc. ... Research Agreement with The University of Pennsylvania ("PENN") ... patients.  The funding will be used to assess ... outcomes in cancer patients undergoing a variety of ... to support the design of a therapeutic, decision-making ...
(Date:6/24/2016)... , ... June 24, 2016 , ... While the majority ... as the Cary 5000 and the 6000i models are higher end machines that use ... height of the spectrophotometer’s light beam from the bottom of the cuvette holder. ...
(Date:6/23/2016)... 2016   Boston Biomedical , an industry ... to target cancer stemness pathways, announced that its ... Drug Designation from the U.S. Food and Drug ... including gastroesophageal junction (GEJ) cancer. Napabucasin is an ... cancer stemness pathways by targeting STAT3, and is ...
(Date:6/23/2016)... MA (PRWEB) , ... June 23, 2016 , ... ... Peel Plate® YM (Yeast and Mold) microbial test has received AOAC Research Institute ... platform of microbial tests introduced last year,” stated Bob Salter, Vice President of ...
Breaking Biology Technology: