Navigation Links
Stem cell proliferation and differentiation observed within hydrogel
Date:4/11/2013

Stem cells can be coaxed to grow into new bone or new cartilage better and faster when given the right molecular cues and room inside a water-loving gel, researchers at Case Western Reserve University show.

By creating a three-dimensional checkerboardone with alternating highly connected and less connected spaces within the hydrogelthe team found adjusting the size of the micropattern could affect stem cell behaviors, such as proliferation and differentiation.

Inducing how and where stem cells growand into the right kind of cell in three dimensionshas proven a challenge to creating useful stem cell therapies. This technique holds promise for studying how physical, chemical and other influences affect cell behavior in three-dimensions, and, ultimately, as a method to grow tissues for regenerative medicine applications.

"We think that control over local biomaterial properties may allow us to guide the formation of complex tissues," said Eben Alsberg, an associate professor of Biomedical Engineering at Case Western Reserve. "With this system, we can regulate cell proliferation and cell-specific differentiation into, for example, bone-like or cartilage-like cells."

Oju Jeon, PhD, a postdoctoral researcher in Biomedical Engineering, pursued this work with Alsberg. Their work is described April 11, 2013 in the online edition of Advanced Functional Materials.

Hydrogels are hydrophilic three-dimensional networks of water-soluble polymers bonded, or crosslinked, to one another. Crosslinks increase rigidity and alter the porous structure inside the gel.

Alsberg and Jeon used a hydrogel of oxidized methacrylated alginate and an 8-arm poly(ethylene glycol) amine. A chemical reaction between the alginate and the poly(ethylene glycol) creates crosslinks that provide structure within the gel.

They tweaked the mix so that a second set of crosslinks forms when exposed to light. They used checkerboard masks to create patterns of alternating singly and doubly crosslinked spaces.

The spaces, which varied in size at 25, 50, 100 and 200 micrometers across, were evenly singly and doubly crosslinked.

Human stem cells isolated from fat tissue were encapsulated in the singly and doubly crosslinked regions. The doubly-crosslinked spaces are comparatively cluttered with structures. The cells grew into clusters in the singly-crosslinked regions, but remained mostly isolated in the doubly crosslinked regions.

The larger the spaces in the checkerboard, the larger the clusters grew.

Cells were cultivated in media that promote differentiation into either bone or cartilage.

In both the singly and doubly crosslinked spaces, stem cells increasingly differentiated according to the media composition as the space size increased. The results were more dramatic in the singly-crosslinked spaces.

"Potentially, what's happening is the single-crosslinked regions allow better nutrient transport and provide more space for cells to interact and, because it's less restrictive, there's space for new cells and matrix production," Alsberg said. "Cluster formation, in turn, may influence proliferation and differentiation. Differences in mechanical properties between regions likely also regulate the cell behaviors."

The researchers are continuing to use micropatterning to understand the influences of biomaterials on stem cell fate decisions. This approach may permit local control over cell behavior and, ultimately, allow the engineering of complex tissues comprised of multiple cell types using a single stem cell source.


'/>"/>

Contact: Kevin Mayhood
kevin.mayhood@case.edu
216-368-4442
Case Western Reserve University
Source:Eurekalert

Related biology news :

1. Successful stem cell differentiation requires DNA compaction, study finds
2. The first ant methylomes uncover the relationship between DNA methylation and caste differentiation
3. Warmest spring on record causes earliest flowering ever observed in eastern U.S.
4. Boston Healthcare Associates Roundtable Explores Challenges and Opportunities Surrounding the Value of Big Data within the Life Sciences
5. New study suggests that Arabica coffee could be extinct in the wild within 70 years
6. New type of bacterial protection found within cells
7. One law to rule them all -- sizes within a species appear to follow a universal distribution
8. Morphing DNA hydrogel flows like liquid but remembers its original shape
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/7/2017)... -- Brandwatch , the leading social intelligence company, today announces ... to uncover insights to support its reporting, help direct future campaigns, ... leading youth charity will be using Brandwatch Analytics social listening and ... understanding of the topics and issues that are a priority for ... "Until ...
(Date:3/2/2017)... March 2, 2017 Who risk to be ... Download the full report: https://www.reportbuyer.com/product/4313699/ WILL ... SENSOR FIELD? Fingerprint sensors using capacitive technology represent ... sensor vendor Idex forecasts an increase of 360% of ... and of the fingerprint sensor market between 2014 and ...
(Date:2/28/2017)...  EyeLock LLC, ein marktführendes Unternehmen im Bereich ... Lösung zur Iris-Erkennung auf der neuesten Mobilplattform ... dem Mobile World Congress 2017 (27. Februar ... 3, Stand 3E10, vorstellen. Der ... – eine Kombination aus Hardware, Software und ...
Breaking Biology News(10 mins):
(Date:3/23/2017)... March 23, 2017  BioPharmX Corporation (NYSE MKT: ... the dermatology market, today reported financial results for ... and will provide an update on the company,s ... "We are pleased to report that ... BioPharmX," said President Anja Krammer. "We achieved key ...
(Date:3/23/2017)... , March 23, 2017 NetworkNewsWire Editorial ... ... putting significant strain on health care systems, in terms of ... rises, so too does the development of innovative and efficient ... effects. Among the many types of cancer treatments, a growing ...
(Date:3/23/2017)... NEW YORK , March 23, 2017 ... closer look at four equities in the Biotech industry: ... EYEG), Synthetic Biologics Inc. (NYSE MKT: SYN), and Regulus ... March 21 st , 2017, Credit Suisse upgraded its rating ... stocks by downloading their free report at: ...
(Date:3/22/2017)... ... March 22, 2017 , ... Researchers face a fundamental ... samples to full-size tissues, bones, even whole organs to implant in people to ... blood deep into the developing tissue. , Current bioengineering techniques, including 3-D ...
Breaking Biology Technology: