Navigation Links
Stealthy microscopy method visualizes E. coli sub-cellular structure in 3-D
Date:6/29/2012

el algorithm to further extend the three-dimensional capabilities by performing deconvolution on the measured 3D field, based on modeling the image using sparsity principles. This microscopy capability, called dSLIT, was used to visualize coiled sub-cellular structures in E. coli cells.

The researchers said that these structures have only been observed using specialized strains and plasmids and fluorescence techniques, and usually on non-living cells. These new methods provide a practical way for non-invasive study of such structures.

Mustafa Mir is first author on the paper and member of Popescu's Quantitative Light Imaging Laboratory at Beckman. Mir said that studying and understanding the three-dimensional internal structure of living cells is essential for furthering our understanding of biological function.

"Visualizing them is extremely challenging due to their small size and transparent nature," Mir said. "This new method, however, provides a way to take advantage of the intrinsic properties of these very small, transparent cells non-invasively and without the use of fluorescence techniques and contrast agents.

"Previous studies have thus used extrinsic contrast such as fluorescence and specialized strains in combination with complex superresolution techniques for such studies. This will allow biologists to study sub-cellular structures while minimally perturbing the cell from its natural state."

The researchers wrote that the method addresses two major issues in cell microscopy: lack of contrast, due to the thin and optically transparent nature of cells, and diffraction limited resolution.

"Although several such structures have been previously identified, little is known about their function and behavior due to the practical difficulties involved in imaging them," they concluded. "The results presented here indicate that dSLIT can be used to characterize and study such sub-cellular structure in a
'/>"/>

Contact: Sue Johnson
johnso16@illinois.edu
217-244-7114
Beckman Institute for Advanced Science and Technology
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UCSB researchers find a way to detect stealthy, hypervirulent Salmonella strains
2. Evaluation of microscopy techniques may help scientists to better understand ancient plants
3. ORNL microscopy yields first proof of ferroelectricity in simplest amino acid
4. Researchers develop new method for analyzing cell function
5. New method generates cardiac muscle patches from stem cells
6. New drug-screening method yields long-sought anti-HIV compounds
7. UTMB researchers create powerful new method to analyze genetic data
8. Healing Method from Australia Addresses the Human Genome
9. A new method detects traces of veterinary drugs in baby food
10. China poised to accept first-ever non-animal test method for cosmetics
11. Chemical engineers at UMass Amherst find high-yield method of making xylene from biomass
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/20/2014)... University of Tennessee, Knoxville, research finds life can persist ... professor was part of a team that examined waters ... Antarctic ice sheet and found the extreme environment supports ... by Jill Mikucki and her colleagues has implications for ... in the solar system. The findings are published in ...
(Date:8/20/2014)... VALLEY, Calif., August 19, 2014 -- Bay Area Lyme ... diagnose and simple to cure, applauds new research published ... journal Ticks and Tick-borne Diseases . The ... Northwest California are active throughout the year, making the ... by researchers at California Department of Public Health (CDPH) ...
(Date:8/20/2014)... chili pepper causes a burning spiciness that is irresistible ... chili pepper,s effect are using their findings to develop ... which can be caused by inflammation or other problems. ... being tested in clinical trials, in ACS, Journal ... explain that decades ago, scientists had pegged a compound ...
Breaking Biology News(10 mins):University of Tennessee research uncovers subglacial life beneath Antarctic ice sheet 2Lyme disease risk is year-round in Northwest California, according to new study 2Lyme disease risk is year-round in Northwest California, according to new study 3
... by LSU Associate Professors of Biological Sciences Fernando Galvez and ... and laboratory study showing the effects of the Deepwater Horizon ... , The study, funded in part by the National Science ... is being published in the journal Proceedings of the ...
... Alzheimer,s disease rises and falls in the spinal fluid in ... Washington University School of Medicine in St. Louis have found. ... and reinforces a link between increased Alzheimer,s risk and inadequate ... brain,s relative inactivity during sleep may provide an opportunity to ...
... Steve Bergens and his graduate student Jeremy Johns ... to revolutionise the chemical industry by reducing its ... Their findings were published in a top international ... the chemical industry with a potential solution to ...
Cached Biology News:LSU researchers find impact of oil spill in marsh fish species 2Marker for Alzheimer's disease rises during day and falls with sleep 2Marker for Alzheimer's disease rises during day and falls with sleep 3Catalyst discovery has potential to revolutionize chemical industry 2
(Date:8/21/2014)... , Aug. 21, 2014 OTC ... Pharmaceuticals Inc., a biotechnology company, on its approval ... previously traded on OTCQX®, the best marketplace for ... Markets Group. Logo - http://photos.prnewswire.com/prnh/20110118/MM31963LOGO ... the successful execution of its growth strategy and ...
(Date:8/21/2014)... August 21, 2014 Gallus ... contract development and manufacturing organization (CMO) announced today ... Inc., an oncology company focused on the development ... to manufacture the anti-prostate specific membrane antigen (PSMA) ... candidate. Under the agreement the antibody will ...
(Date:8/20/2014)... lessons often have it that American Indians largely were ... pox, influenza and tuberculosis brought to the New World ... about 20 million people lived in the Americas shortly ... were killed by European diseases., But new research led ... and Johannes Krause of the University of Tubingen in ...
(Date:8/20/2014)... 2014  PAREXEL International Corporation (NASDAQ: PRXL ... the Baird Healthcare Conference in New York.  Ingo ... will be making a presentation on PAREXEL and discussing ... 3, 2014. A live webcast of the ... PAREXEL,s website at www.PAREXEL.com in the Upcoming ...
Breaking Biology Technology:OTC Markets Group Congratulates Immune Pharmaceuticals on NASDAQ Listing 2Gallus Enters Agreement with Progenics Pharmaceuticals, Inc. for Manufacture of Anti-PSMA Monoclonal Antibody 2Gallus Enters Agreement with Progenics Pharmaceuticals, Inc. for Manufacture of Anti-PSMA Monoclonal Antibody 3Unusual discovery leads to fascinating tuberculosis theory 2Unusual discovery leads to fascinating tuberculosis theory 3PAREXEL International To Present At Baird Healthcare Conference 2
... Life Sciences,Holdings, Inc. (Nasdaq: ADLS ), ... and commercialization of novel drugs in the,therapeutic ... today,announced that it was added to the ... reconstituted its comprehensive set of U.S. and,global ...
... 3 /Xinhua-PRNewswire-FirstCall/ -- Huifeng,Bio-Pharmaceutical Technology, Inc. (OTC ... of plant extracts and pharmaceutical raw materials,for ... in China,announced today that its company information ... Market Access Program, an information distribution,service that ...
... to offer choice of two distinct drugs on ... separate drug-eluting stent platforms, NATICK, ... today announced that the U.S. Food and Drug,Administration (FDA) has ... coronary artery disease. The,PROMUS Stent is a private-labeled XIENCE(TM) V ...
Cached Biology Technology:Advanced Life Sciences Added to Russell Microcap Index 2Advanced Life Sciences Added to Russell Microcap Index 3Huifeng Bio-Pharmaceutical (HFGB) Information to Be Available Through Standard & Poor's Market Access Program 2Boston Scientific Announces FDA Approval for PROMUS(TM) Everolimus-Eluting Coronary Stent System 2Boston Scientific Announces FDA Approval for PROMUS(TM) Everolimus-Eluting Coronary Stent System 3Boston Scientific Announces FDA Approval for PROMUS(TM) Everolimus-Eluting Coronary Stent System 4
Piperazine-N',N'-bis(2-ethanesulfonic acid), monosodium salt...
... receptors constitute the principal excitatory ... classes of glutamate receptors exist: ... Ionotropic glutamate receptors are oligomeric ... NMDA1-3, KAI-2) which comprise ligand-gated ...
Recommended fro Western blotting application...
... Anti-Krueppel-like factor 15 (KLF15) KLF15 is ... found abundantly expressed in the liver, kidneys, ... NP_054798 Specificity: Polyclonal antibody produced in ... to a region of human KLF15 with ...
Biology Products: