Navigation Links
Speeding up drug discovery with rapid 3-D mapping of proteins
Date:5/30/2012

hey had a therapeutic effect. Given a blueprint of the 3D structure of a hIMP involved in a specific disease, however, drug developers could focus only on molecules that are most likely to interact with the target hIMP, saving time and expense.

In the past, it was extremely difficult to solve the structure of hIMPs, due to the difficulty of harvesting them from cells and the difficulty of labeling the amino acids that compose the proteins, a key step in determining their three-dimensional configuration.

"One problem was that hIMPs serve many functions in a cell, so if you tried to engineer cells with many copies of the proteins on their membrane, they would die before you could harvest the hIMPs," says Christian Klammt, a postdoctoral researcher in Choe's lab and a first author on the paper.

To get around this, the scientists created an outside-the-cell environment, called cell-free expression system, to synthesize the proteins. They used a plexiglass chamber that contained all the biochemical elements necessary to manufacture hIMPs as if they were inside the cell. This system provided the researchers with enough of the proteins to conduct structural analysis.

The cell-free method also allowed them to easily add labeled amino acids into the biochemical stew, which were then incorporated into the proteins. These amino acids gave off telltale structural clues when analyzed with nuclear magnetic resonance spectroscopy, a method for using the magnetic properties of atoms to determine a molecule's physical and chemical properties.

"It was very difficult and inefficient to introduce labeled amino acids selectively into the protein produced in live cells," says Innokentiy Maslennikov, a Salk staff scientist and co-first author on the paper. "With a cell-free system, we can precisely control what amino acids are available for protein production, giving us isotope-labeled hIMPs in large quantities. Using a proprietary labeling str
'/>"/>

Contact: Andy Hoang
ahoang@salk.edu
619-861-5811
Salk Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Systems biology brings hope of speeding up drug development
2. Graphene may hold key to speeding up DNA sequencing
3. Chemical equator discovery will aid pollution mapping
4. Sirtris review of sirtuin therapeutics for diseases of aging in Nature Reviews Drug Discovery
5. Groundbreaking discovery may lead to stronger antibiotics
6. Discovery of natural compounds that could slow blood vessel growth
7. Nanoscopic screening process to speed drug discovery
8. FSU researchers discovery leads to $1.5 million grant, potential new treatment of liver fibrosis
9. New $11 million center to speed production of new compounds for drug discovery
10. Discovery of giant roaming deep sea protist provides new perspective on animal evolution
11. New discovery may enhance MRI scans, lead to portable MRI machines
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Speeding up drug discovery with rapid 3-D mapping of proteins
(Date:12/17/2014)... -- Valencell, a leader in performance biometric data sensor ... leaders such as Intel, Jabra, Atlas, and Scosche to ... These products will be showcased at CES 2015, taking ... . "Our partners choose PerformTek biometrics, ... with the clinical data to back it up," said ...
(Date:12/11/2014)... Research and Markets , ... http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has announced the addition of the ... to their offering. One major ... of multimodal biometric systems. Multimodal biometric systems utilize ... verification and identification purposes. This helps to reduce ...
(Date:12/10/2014)... Dec. 9, 2014  Wake Forest Baptist Medical Center today ... its School of Medicine. Funding for this $50 million ... that will be publicly launched next summer. ... the former 60 series R.J. Reynolds Tobacco Company complex, ... will begin immediately with plans to be ready to ...
Breaking Biology News(10 mins):Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4
... fish is good for our brains and memory. But what ... fish, that makes our memory sharper? Medical researchers at ... published their findings in the peer-reviewed journal Applied Physiology, ... his team discovered lab models fed a high-DHA diet had ...
... Steve Moose, an associate professor of maize functional genomics at ... think they may have discovered a new source of heterosis, ... small RNAs (sRNAs), a class of double-stranded RNA molecules that ... refers to the increased vigor or general health, resistance to ...
... the Royal Astronomical Society (RAS) announced a new publishing ... prestigious journals. OUP will assume publication of ... Monthly Notices of the Royal Astronomical Society: Letters , ... Geophysics from January 2013. Professor David Southwood, ...
Cached Biology News:A new source of maize hybrid vigor 2A new source of maize hybrid vigor 3Royal Astronomical Society and Oxford University Press partnership announced 2
(Date:12/19/2014)... Altamonte Springs, FL (PRWEB) December 18, 2014 ... one of the nation’s leading specialty pharmacies, has ... who serve as clinical pharmacy consultants for prescriber ... disease therapy expertise. , “We have a ... disease therapies. For example, those specializing in hepatitis ...
(Date:12/19/2014)... Reports from CDC show approximately 117 ... or more chronic health conditions - including cardiovascular disease, ... of these diseases, but may not eliminate the severe ... implanted nerve stimulation devices today offer relief, they require ... induce side effects. , To eliminate the need ...
(Date:12/19/2014)... CA (PRWEB) December 18, 2014 With ... limited time special on Sartorius Biohit products . ... on mLINE pipettes, Picus Electronic Pipette Trade-in Program, and ... Health and Safety officers around the world. They are ... pipetting. Other features include:, ,     Full volume ...
(Date:12/19/2014)... YORK , Dec. 18, 2014 Relmada ... therapies for the treatment of chronic pain, announced the ... today. "This was a transformational year in ... promising future for our company," said Sergio Traversa ... year, we have executed on key financial, human resource ...
Breaking Biology Technology:BioPlus Specialty Pharmacy Expands ‘Regional Pharmaceutical Care Liaison’ Team 2BioPlus Specialty Pharmacy Expands ‘Regional Pharmaceutical Care Liaison’ Team 3Draper Wins GSK Funding for Smaller, Smarter Implantable Devices 2Major Pipette Distributor Pipette.com Announces Sartorius Year End Specials 2Relmada Therapeutics Announces Results of Annual Shareholders Meeting 2
... 7, 2011 DuPont (NYSE: DD ) ... at the JPMorgan Diversified Industries Conference to expect attractive ... selective investment in attractive areas, broad-based growth, especially in ... also reaffirmed DuPont,s 2011 earnings per share (EPS) outlook ...
... June 7, 2011 Infrared Systems International (OTCBB: IFRS) is ... a pilot study of its NatuRx™ HIV/AIDS treatment and will ... AquaLiv concluded a pilot study with two HIV/AIDS ... anniversary of the first reported AIDS victims on June 5th, ...
... its origins in Hamburg. Jrg Weimller, a materials scientist ... Helmholtz Center Geesthacht, has carried out research on this ... Institute for Metal Research in Shenyang, China., The 51-year-old ... which opens the door to a multitude of diverse ...
Cached Biology Technology:DuPont Chair & CEO: Delivering Innovative Solutions and Long-term Growth 2DuPont Chair & CEO: Delivering Innovative Solutions and Long-term Growth 3Infrared Systems International's Subsidiary, AquaLiv, Inc., Strives to Be a Game Changer in Fight Against HIV/AIDS 2Infrared Systems International's Subsidiary, AquaLiv, Inc., Strives to Be a Game Changer in Fight Against HIV/AIDS 3Hard or soft: At the touch of a button 2Hard or soft: At the touch of a button 3
Recombinant Mouse Serpin A1/alpha-1-Antitrypsin, CF Protein Family: Coagulation, Serine Protease Regulators...
ANTI S. GROUP AG:H...
This kit only detects beta-galactosidase activity at pH 6 in cultured cells andtissue sections. Beta-galactosidase activity at pH 6 ispresent only in senescent cells, and is not found inpresenescent...
2,4,6, Trinitrophenyl hapten is conjugated to lipopolysaccharide (LPS)....
Biology Products: